In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Esin AKBAY; Y. Doruk ARACAGÖK; Ali Hakan GÖKER; Mehmet Ali ONUR; Nilüfer CİHANGİR

doi:https://doi.org/10.15671/hjbc.680564

Article Details

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Authors:

Esin AKBAY | Y. Doruk ARACAGÖK | Ali Hakan GÖKER | Mehmet Ali ONUR | Nilüfer CİHANGİR

Publisher:

Hacettepe University

Main Topics:

Applied physics

Journal:

Hacettepe Journal of Biology and Chemistry 2021 Vol.49 Issue 1, pp.25-36

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2000448169

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X

DOI:

https://doi.org/10.15671/hjbc.680564

Abstract:

 

The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by MTT, while apoptosis was determined by AO/PI double staining method. The control group was free from any agent (DMEM only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.

Article Details

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Authors: Esin AKBAY | Y. Doruk ARACAGÖK | Ali Hakan GÖKER | Mehmet Ali ONUR | Nilüfer CİHANGİR

Publisher: Hacettepe University

Main Topics: Applied physics

Journal: Hacettepe Journal of Biology and Chemistry 2021 Vol.49 Issue 1, pp.25-36

Document Type: Article

Publication Date: 12/2020

Document Language: English

Document ID (Org No.): 2000448169

Frequency: Quarterly

eISSN: 2687-475X

pISSN: 2687-475X

DOI: https://doi.org/10.15671/hjbc.680564

Issues

Authors:

Esin AKBAY | Y. Doruk ARACAGÖK | Ali Hakan GÖKER | Mehmet Ali ONUR | Nilüfer CİHANGİR

Publisher:

Hacettepe University

Main Topics:

Applied physics

Journal:

Hacettepe Journal of Biology and Chemistry 2021 Vol.49 Issue 1, pp.25-36

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2000448169

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X

DOI:

https://doi.org/10.15671/hjbc.680564