Apocynin exhibits an ameliorative effect on endothelial dysfunction/ atherosclerosis-related factors in high-fat diet-induced obesity in rats

Nurdan Bülbül Aycı; Büşra Ertaş; Gökçe Güllü Amuran; Feriha Ercan; Göksel Şener; Oya Orun; Mustafa Akkiprik

Article Details

Apocynin exhibits an ameliorative effect on endothelial dysfunction/ atherosclerosis-related factors in high-fat diet-induced obesity in rats

Authors:

Nurdan Bülbül Aycı | Büşra Ertaş | Gökçe Güllü Amuran | Feriha Ercan | Göksel Şener | Oya Orun | Mustafa Akkiprik

Publisher:

Marmara University

Main Topics:

Surgery (Other)

Journal:

Marmara Medical Journal Vol.37 Issue 2, pp.238-247

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001317361

pISSN:

1019-1941

Keywords:

Abstract:

 Objective: The aim of this study was to reveal the effect of apocynin APO on the factors involved in obesity-related endothelial dysfunction ED and atherosclerosis AS. Materials and Methods: Male Wistar albino rats were divided into control CNT, high-fat diet HFD and HFDAPO groups. HFD and HFDAPO groups were fed HFD for sixteen weeks. APO 25 mgkg was administered to the HFDAPO group for the last four weeks. The effects of APO on: AS-related metabolic parameters triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin, oxidative stress OS,  malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate NADPH- oxidase-2, oxidised-low-density lipoprotein ox-LDL and 8-hydroxy-2-deoxyguanosine, low-density lipoprotein and ox-LDL uptake potential activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively, tissue inflammation myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha, ED endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide, programmed cell death terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1, smooth muscle cell transformation alpha-smooth muscle actin, histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and ox- LDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.

 Objective: The aim of this study was to reveal the effect of apocynin APO on the factors involved in obesity-related endothelial dysfunction ED and atherosclerosis AS. Materials and Methods: Male Wistar albino rats were divided into control CNT, high-fat diet HFD and HFDAPO groups. HFD and HFDAPO groups were fed HFD for sixteen weeks. APO 25 mgkg was administered to the HFDAPO group for the last four weeks. The effects of APO on: AS-related metabolic parameters triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin, oxidative stress OS,  malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate NADPH- oxidase-2, oxidised-low-density lipoprotein ox-LDL and 8-hydroxy-2-deoxyguanosine, low-density lipoprotein and ox-LDL uptake potential activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively, tissue inflammation myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha, ED endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide, programmed cell death terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1, smooth muscle cell transformation alpha-smooth muscle actin, histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and ox- LDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.

 Objective: The aim of this study was to reveal the effect of apocynin APO on the factors involved in obesity-related endothelial dysfunction ED and atherosclerosis AS. Materials and Methods: Male Wistar albino rats were divided into control CNT, high-fat diet HFD and HFDAPO groups. HFD and HFDAPO groups were fed HFD for sixteen weeks. APO 25 mgkg was administered to the HFDAPO group for the last four weeks. The effects of APO on: AS-related metabolic parameters triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin, oxidative stress OS,  malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate NADPH- oxidase-2, oxidised-low-density lipoprotein ox-LDL and 8-hydroxy-2-deoxyguanosine, low-density lipoprotein and ox-LDL uptake potential activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively, tissue inflammation myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha, ED endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide, programmed cell death terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1, smooth muscle cell transformation alpha-smooth muscle actin, histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and ox- LDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.

 Objective: The aim of this study was to reveal the effect of apocynin APO on the factors involved in obesity-related endothelial dysfunction ED and atherosclerosis AS. Materials and Methods: Male Wistar albino rats were divided into control CNT, high-fat diet HFD and HFDAPO groups. HFD and HFDAPO groups were fed HFD for sixteen weeks. APO 25 mgkg was administered to the HFDAPO group for the last four weeks. The effects of APO on: AS-related metabolic parameters triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin, oxidative stress OS,  malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate NADPH- oxidase-2, oxidised-low-density lipoprotein ox-LDL and 8-hydroxy-2-deoxyguanosine, low-density lipoprotein and ox-LDL uptake potential activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively, tissue inflammation myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha, ED endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide, programmed cell death terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1, smooth muscle cell transformation alpha-smooth muscle actin, histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and ox- LDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.

Article Details

Apocynin exhibits an ameliorative effect on endothelial dysfunction/ atherosclerosis-related factors in high-fat diet-induced obesity in rats

Authors:

Nurdan Bülbül Aycı | Büşra Ertaş | Gökçe Güllü Amuran | Feriha Ercan | Göksel Şener | Oya Orun | Mustafa Akkiprik

Publisher:

Marmara University

Main Topics:

Surgery (Other)

Journal:

Marmara Medical Journal Vol.37 Issue 2, pp.238-247

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001317361

pISSN:

1019-1941

Apocynin

Atherosclerosis

Endothelial dysfunction

Oxidative stress

Obesity

High-fat diet

Issues

Article Details

Apocynin exhibits an ameliorative effect on endothelial dysfunction/ atherosclerosis-related factors in high-fat diet-induced obesity in rats

Authors: Nurdan Bülbül Aycı | Büşra Ertaş | Gökçe Güllü Amuran | Feriha Ercan | Göksel Şener | Oya Orun | Mustafa Akkiprik

Publisher: Marmara University

Main Topics: Surgery (Other)

Journal: Marmara Medical Journal Vol.37 Issue 2, pp.238-247

Document Type: Article

Publication Date: 5/2024

Document Language: English

Document ID (Org No.): 2001317361

pISSN: 1019-1941

Apocynin

Atherosclerosis

Endothelial dysfunction

Oxidative stress

Obesity

High-fat diet

Issues

Authors:

Nurdan Bülbül Aycı | Büşra Ertaş | Gökçe Güllü Amuran | Feriha Ercan | Göksel Şener | Oya Orun | Mustafa Akkiprik

Publisher:

Marmara University

Main Topics:

Surgery (Other)

Journal:

Marmara Medical Journal Vol.37 Issue 2, pp.238-247

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001317361

pISSN:

1019-1941