Production of an E. coli Toxin Protein; Colicin A in E. coli Using an Inducible System

İsa GÖKÇE

Article Details

Production of an E. coli Toxin Protein; Colicin A in E. coli Using an Inducible System

Authors:

İsa GÖKÇE

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2003 Vol.27 Issue 3, pp.323-332

Document Type:

Article

Publication Date:

2/2003

Document Language:

Turkish

Document ID (Org No.):

2000729581

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527

Abstract:

 

Colicins are bacterial toxins that kill Escherichia coli and  related cells; their mode of action is of interest in protein import  and toxicology.  Colicins translocate across the E. coli outer  membrane and periplasm by interacting with several receptors.  This  translocation process involves interaction of the colicin with the  outer membrane porin OmpF and subsequently with the integral membrane  protein TolA. The N-terminal domain of colicin N is involved in the  import process.  Our aim was to produce a large quantity of colicin A  for functional and structural studies.  It is a prerequisite to have a  correctly folded and stable protein for these studies.  The commonly  utilised expression system uses the Lex A promoter, which requires  induction with toxic mitomycin C, though the yield is low.  Here we  present the production of an E. coli toxin and its immunity protein in  E. coli using a safe inducible promoter.

 

Colicins are bacterial toxins that kill Escherichia coli and  related cells; their mode of action is of interest in protein import  and toxicology.  Colicins translocate across the E. coli outer  membrane and periplasm by interacting with several receptors.  This  translocation process involves interaction of the colicin with the  outer membrane porin OmpF and subsequently with the integral membrane  protein TolA. The N-terminal domain of colicin N is involved in the  import process.  Our aim was to produce a large quantity of colicin A  for functional and structural studies.  It is a prerequisite to have a  correctly folded and stable protein for these studies.  The commonly  utilised expression system uses the Lex A promoter, which requires  induction with toxic mitomycin C, though the yield is low.  Here we  present the production of an E. coli toxin and its immunity protein in  E. coli using a safe inducible promoter.

Article Details

Production of an E. coli Toxin Protein; Colicin A in E. coli Using an Inducible System

Authors: İsa GÖKÇE

Publisher: TUBITAK

Journal: Turkish Journal of Chemistry 2003 Vol.27 Issue 3, pp.323-332

Document Type: Article

Publication Date: 2/2003

Document Language: Turkish

Document ID (Org No.): 2000729581

Frequency: Bimonthly

eISSN: 1303-6130

pISSN: 1300-0527

Issues

Authors:

İsa GÖKÇE

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2003 Vol.27 Issue 3, pp.323-332

Document Type:

Article

Publication Date:

2/2003

Document Language:

Turkish

Document ID (Org No.):

2000729581

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527