An Analysis of Micronuclei and DNA Damage Induced by Methotrexate Treatment of Male Albino Mice

Nagla Zaky Ibrahim El-Alfy; Amany Ibrahim Alqosaibi; Mahmoud Fathy Mahmoud; Sally Ramadan Gabr El-Ashry

doi:10.12816/0033759

Article Details

An Analysis of Micronuclei and DNA Damage Induced by Methotrexate Treatment of Male Albino Mice

Authors:

Nagla Zaky Ibrahim El-Alfy | Amany Ibrahim Alqosaibi | Mahmoud Fathy Mahmoud | Sally Ramadan Gabr El-Ashry

Publisher:

Pan Arab League of Continuous Medical Education

Main Topics:

Medicine

Journal:

The Egyptian Journal of Hospital Medicine 2016 Vol.65, pp.504-514

Document Type:

Article

Publication Date:

10/2016

Document Language:

English

Document ID (Org No.):

97180

Frequency:

Quarterly

eISSN:

20907125

pISSN:

16872002

DOI:

10.12816/0033759

Descriptors:

Keywords:

Abstract:

  Background: Methotrexate is an antineoplastic, antipsoriatic and antirheumatic agent belongs to the group of antimetabolites and inhibits folic acid metabolism. Materials and methods: To investigate its possible effect, sixty male mice were randomly assigned to one of four groups (one control and three treated groups with different doses of methotrexate). Mice of groups 1, 2 and 3 were intraperitoneally injected with 2.5, 5 and 10 mg /kg b.wt. methotrexate respectively. All the control and treated animals were sacrificed at 24, 48 and 72 hour by cervical dislocation post treatment. Results: Micronucleus assay results showed that methotrexate treatment induced genotoxicity in bone marrow cells, the number of micronucleated polychromatic erythrocytes (MNPCEs) and the ratio of polychromatic erythrocytes / normochromatic erythrocytes was gradually increased significantly (P < 0.001) by increasing dose and time of treatment in methotrexate treated groups in comparison with the control group. An analysis of randomly amplified polymorphism DNA-polymerase chain reaction (RAPD-PCR) showed different ranges of DNA modifications in the treated groups after 24, 48 and 72 hour of treatment in comparison with the control group. Results of this study indicate that methotrexate treatment induced cytotoxic and genotoxic effect on bone marrow cells and DNA content of male albino mice even after a low dose and single treatment. Conclusion: Therefore, the therapeutic uses of methotrexate should be restricted to a very narrow range border.

Article Details

An Analysis of Micronuclei and DNA Damage Induced by Methotrexate Treatment of Male Albino Mice

Authors:

Nagla Zaky Ibrahim El-Alfy | Amany Ibrahim Alqosaibi | Mahmoud Fathy Mahmoud | Sally Ramadan Gabr El-Ashry

Publisher:

Pan Arab League of Continuous Medical Education

Main Topics:

Medicine

Journal:

The Egyptian Journal of Hospital Medicine 2016 Vol.65, pp.504-514

Document Type:

Article

Publication Date:

10/2016

Document Language:

English

Document ID (Org No.):

97180

Frequency:

Quarterly

eISSN:

20907125

pISSN:

16872002

DOI:

10.12816/0033759

Descriptors:

DNA

ANALYSIS SAMPLES

ANTIMETABOLITES

DAMAGE

MICE

LABORATORY ANIMALS

BIOLOGY

DNA

Mice

Methotrexate

Micronuclei

RAPD - PCR

Issues

Article Details

Authors: Nagla Zaky Ibrahim El-Alfy | Amany Ibrahim Alqosaibi | Mahmoud Fathy Mahmoud | Sally Ramadan Gabr El-Ashry

Publisher: Pan Arab League of Continuous Medical Education

Main Topics: Medicine

Journal: The Egyptian Journal of Hospital Medicine 2016 Vol.65, pp.504-514

Document Type: Article

Publication Date: 10/2016

Document Language: English

Document ID (Org No.): 97180

Frequency: Quarterly

eISSN: 20907125

pISSN: 16872002

DOI: 10.12816/0033759

Descriptors:

Issues

Authors:

Nagla Zaky Ibrahim El-Alfy | Amany Ibrahim Alqosaibi | Mahmoud Fathy Mahmoud | Sally Ramadan Gabr El-Ashry

Publisher:

Pan Arab League of Continuous Medical Education

Main Topics:

Medicine

Journal:

The Egyptian Journal of Hospital Medicine 2016 Vol.65, pp.504-514

Document Type:

Article

Publication Date:

10/2016

Document Language:

English

Document ID (Org No.):

97180

Frequency:

Quarterly

eISSN:

20907125

pISSN:

16872002

DOI:

10.12816/0033759