Application of Potassium Chromate-Diphenylcarbazide in the Quantitative Determination of Ascorbic Acid by Spectrophotometry

Meissam NOROOZIFAR; Mozhgan KHORASANI-MOTLAGH

Article Details

Application of Potassium Chromate-Diphenylcarbazide in the Quantitative Determination of Ascorbic Acid by Spectrophotometry

Authors:

Meissam NOROOZIFAR | Mozhgan KHORASANI-MOTLAGH

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2003 Vol.27 Issue 6, pp.717-722

Document Type:

Article

Publication Date:

5/2003

Document Language:

Turkish

Document ID (Org No.):

2000729457

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527

Abstract:

 

A spectrophotometric procedure for the determination of  ascorbic acid in pure form and in a number of pharmaceutical  preparations and real samples has been developed that offers the  advantages of simplicity, accuracy, precision and sensitivity over  many other methods.  The method is based on the oxidation of ascorbic  acid by a known excess amount of potassium chromate followed by the  estimation of the unreacted amount of chromate by reactions with  sym-diphenylcarbazide.  The reacted oxidant corresponds to the  ascorbic content.  At the maximum absorption of 548 nm, Beer's law is  obeyed up to 5 m g/mL of ascorbic acid.  Statistical treatment of  the experimental results indicates that the procedure is precise and  accurate.  Excipients used as additives in pharmaceutical formulations  did not interfere in the proposed procedure.  The reliability of the  method was established by parallel determination against the  2,6-dichlorophenolindophenol methods.  The procedure described was  successfully applied to the determination of bulk drugs, in  pharmaceutical formulations and real samples.

 

A spectrophotometric procedure for the determination of  ascorbic acid in pure form and in a number of pharmaceutical  preparations and real samples has been developed that offers the  advantages of simplicity, accuracy, precision and sensitivity over  many other methods.  The method is based on the oxidation of ascorbic  acid by a known excess amount of potassium chromate followed by the  estimation of the unreacted amount of chromate by reactions with  sym-diphenylcarbazide.  The reacted oxidant corresponds to the  ascorbic content.  At the maximum absorption of 548 nm, Beer's law is  obeyed up to 5 m g/mL of ascorbic acid.  Statistical treatment of  the experimental results indicates that the procedure is precise and  accurate.  Excipients used as additives in pharmaceutical formulations  did not interfere in the proposed procedure.  The reliability of the  method was established by parallel determination against the  2,6-dichlorophenolindophenol methods.  The procedure described was  successfully applied to the determination of bulk drugs, in  pharmaceutical formulations and real samples.

Article Details

Application of Potassium Chromate-Diphenylcarbazide in the Quantitative Determination of Ascorbic Acid by Spectrophotometry

Authors: Meissam NOROOZIFAR | Mozhgan KHORASANI-MOTLAGH

Publisher: TUBITAK

Journal: Turkish Journal of Chemistry 2003 Vol.27 Issue 6, pp.717-722

Document Type: Article

Publication Date: 5/2003

Document Language: Turkish

Document ID (Org No.): 2000729457

Frequency: Bimonthly

eISSN: 1303-6130

pISSN: 1300-0527

Issues

Authors:

Meissam NOROOZIFAR | Mozhgan KHORASANI-MOTLAGH

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2003 Vol.27 Issue 6, pp.717-722

Document Type:

Article

Publication Date:

5/2003

Document Language:

Turkish

Document ID (Org No.):

2000729457

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527