Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures

Gülendem GÜNENDİ; Fahrünnisa PAMUK

Article Details

Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures

Authors:

Gülendem GÜNENDİ | Fahrünnisa PAMUK

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 1999 Vol.23 Issue 3, pp.269-274

Document Type:

Article

Publication Date:

2/1999

Document Language:

Turkish

Document ID (Org No.):

2000730400

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527

Abstract:

 

A new TLC separation and quantitation method for L-tyrosine  and L-Dopa mixtures was developed.  The minimum tyrosine and Dopa  quantities which can be measured by this technique are 0.7 and 1.5 m  g respectively.  The method may be used to measure the kinetic  parameters of polyphenoloxidase as well as to trace the enzyme  catalyzed conversion of L-tyrosine into L-Dopa.  For a set of 20  measurements the maximum difference between any two measurements of  spot areas was found to be 3 mm2 (4.5%) for L-Dopa (4 m g) and  2mm2 (5.6%) for L-tyrosine (1m g).

 

A new TLC separation and quantitation method for L-tyrosine  and L-Dopa mixtures was developed.  The minimum tyrosine and Dopa  quantities which can be measured by this technique are 0.7 and 1.5 m  g respectively.  The method may be used to measure the kinetic  parameters of polyphenoloxidase as well as to trace the enzyme  catalyzed conversion of L-tyrosine into L-Dopa.  For a set of 20  measurements the maximum difference between any two measurements of  spot areas was found to be 3 mm2 (4.5%) for L-Dopa (4 m g) and  2mm2 (5.6%) for L-tyrosine (1m g).

Article Details

Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures

Authors: Gülendem GÜNENDİ | Fahrünnisa PAMUK

Publisher: TUBITAK

Journal: Turkish Journal of Chemistry 1999 Vol.23 Issue 3, pp.269-274

Document Type: Article

Publication Date: 2/1999

Document Language: Turkish

Document ID (Org No.): 2000730400

Frequency: Bimonthly

eISSN: 1303-6130

pISSN: 1300-0527

Issues

Authors:

Gülendem GÜNENDİ | Fahrünnisa PAMUK

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 1999 Vol.23 Issue 3, pp.269-274

Document Type:

Article

Publication Date:

2/1999

Document Language:

Turkish

Document ID (Org No.):

2000730400

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527