Evaluation of anti-Plasmodium berghei activity of crude and column fractions of extracts from Withania somnifera

Zerihun Teklemariam DAME; Beyene PETROS; Yalemtsehay MEKONNEN

Article Details

Evaluation of anti-Plasmodium berghei activity of crude and column fractions of extracts from Withania somnifera

Authors:

Zerihun Teklemariam DAME | Beyene PETROS | Yalemtsehay MEKONNEN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.147-150

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000812469

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Different crude methanolic and chloroform extracts of Withania somnifera leaves, and column fractions of the methanolic extract, were tested in vivo for antimalarial activity on Swiss albino male mice. Each mouse in the study was infected intraperitoneally with 0.2 mL of blood containing 106-107 infected erythrocytes taken from mice previously infected with chloroquine-sensitive Plasmodium berghei. The extracts were given to the infected mice intraperitoneally starting from 3 h following inoculation. Antimalarial activity was evaluated by taking blood smears on day 4. The results revealed that column fractions of methanolic extract obtained from leaves of Withania somnifera showed significantly improved suppression of parasitemia at lower doses compared to the crude extracts. Reduction of parasitemia by 44% and 57% was observed at doses of 200 and 300 mg/kg body weight, respectively, by column fractions of methanolic crude extract.

 

Different crude methanolic and chloroform extracts of Withania somnifera leaves, and column fractions of the methanolic extract, were tested in vivo for antimalarial activity on Swiss albino male mice. Each mouse in the study was infected intraperitoneally with 0.2 mL of blood containing 106-107 infected erythrocytes taken from mice previously infected with chloroquine-sensitive Plasmodium berghei. The extracts were given to the infected mice intraperitoneally starting from 3 h following inoculation. Antimalarial activity was evaluated by taking blood smears on day 4. The results revealed that column fractions of methanolic extract obtained from leaves of Withania somnifera showed significantly improved suppression of parasitemia at lower doses compared to the crude extracts. Reduction of parasitemia by 44% and 57% was observed at doses of 200 and 300 mg/kg body weight, respectively, by column fractions of methanolic crude extract.

Article Details

Evaluation of anti-Plasmodium berghei activity of crude and column fractions of extracts from Withania somnifera

Authors:

Zerihun Teklemariam DAME | Beyene PETROS | Yalemtsehay MEKONNEN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.147-150

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000812469

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Antimalarial activity

in vivo

Plasmodium berghei

Withania somnifera

Issues

Article Details

Evaluation of anti-Plasmodium berghei activity of crude and column fractions of extracts from Withania somnifera

Authors: Zerihun Teklemariam DAME | Beyene PETROS | Yalemtsehay MEKONNEN

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.147-150

Document Type: Article

Publication Date: 1/2013

Document Language: Turkish

Document ID (Org No.): 2000812469

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Antimalarial activity

in vivo

Plasmodium berghei

Withania somnifera

Issues

Authors:

Zerihun Teklemariam DAME | Beyene PETROS | Yalemtsehay MEKONNEN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 2, pp.147-150

Document Type:

Article

Publication Date:

1/2013

Document Language:

Turkish

Document ID (Org No.):

2000812469

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152