Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Özgen Kılıç Erkek; Gülşah Gündoğdu

Article Details

Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Authors:

Özgen Kılıç Erkek | Gülşah Gündoğdu

Publisher:

Pamukkale University

Main Topics:

Tıbbi Fizyoloji (Diğer)

Journal:

Pamukkale Tıp Dergisi Vol.17 Issue 4, pp.690-701

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001315060

pISSN:

1308-0865

Keywords:

Abstract:

 Purpose: The purpose of this study was to assess the effect of alternate-day fasting ADF concerning sex as well as its function in systemic and tissue-level oxidative stress alterations associated with aging. Materials and methods: Forty-two female n21 and male n21 Wistar rats aged 16 months were separated into six groups n7 each: Group-1 control-male, Group-2 1-month, ADF-male, Group-3 2-month, ADFmale, Group-4 control-female, Group-5 1-month, ADF-female, and Group-6 2-month, ADF-female. The ADF protocol was applied every other day for 24-h of fasting three daysweek. Serum samples were analyzed via ELISA to measure total oxidant-antioxidant status TOS-TAS, and the oxidative stress index OSI was calculated. Results: 2-months of ADF treatment reduced body weight BW compared compliance control groups p0.001. All groups cumulative food intake and retroperitoneal fat weight decreased with ADF p0.05. Both 1-month and 2-month ADF interventions had positive effects on reducing TOS and OSI in both liver and serum, with a significant decrease observed in both groups compared to their respective controls p0.001. The liver TAS significantly increased in female rats p0.05, but this increase did not reach a significant level in male rats. The difference in the serum TAS between the groups was not significant. Conclusions: This study evaluated the effects of ADF on BW, food consumption, and oxidative stress parameters in male and female rats. The findings highlight ADFs potential benefits in weight management and reducing oxidative stress. This study represents an important step in understanding the effects of ADF on metabolic health and in identifying potential clinical applications.

 Purpose: The purpose of this study was to assess the effect of alternate-day fasting ADF concerning sex as well as its function in systemic and tissue-level oxidative stress alterations associated with aging. Materials and methods: Forty-two female n21 and male n21 Wistar rats aged 16 months were separated into six groups n7 each: Group-1 control-male, Group-2 1-month, ADF-male, Group-3 2-month, ADFmale, Group-4 control-female, Group-5 1-month, ADF-female, and Group-6 2-month, ADF-female. The ADF protocol was applied every other day for 24-h of fasting three daysweek. Serum samples were analyzed via ELISA to measure total oxidant-antioxidant status TOS-TAS, and the oxidative stress index OSI was calculated. Results: 2-months of ADF treatment reduced body weight BW compared compliance control groups p0.001. All groups cumulative food intake and retroperitoneal fat weight decreased with ADF p0.05. Both 1-month and 2-month ADF interventions had positive effects on reducing TOS and OSI in both liver and serum, with a significant decrease observed in both groups compared to their respective controls p0.001. The liver TAS significantly increased in female rats p0.05, but this increase did not reach a significant level in male rats. The difference in the serum TAS between the groups was not significant. Conclusions: This study evaluated the effects of ADF on BW, food consumption, and oxidative stress parameters in male and female rats. The findings highlight ADFs potential benefits in weight management and reducing oxidative stress. This study represents an important step in understanding the effects of ADF on metabolic health and in identifying potential clinical applications.

 Purpose: The purpose of this study was to assess the effect of alternate-day fasting ADF concerning sex as well as its function in systemic and tissue-level oxidative stress alterations associated with aging. Materials and methods: Forty-two female n21 and male n21 Wistar rats aged 16 months were separated into six groups n7 each: Group-1 control-male, Group-2 1-month, ADF-male, Group-3 2-month, ADFmale, Group-4 control-female, Group-5 1-month, ADF-female, and Group-6 2-month, ADF-female. The ADF protocol was applied every other day for 24-h of fasting three daysweek. Serum samples were analyzed via ELISA to measure total oxidant-antioxidant status TOS-TAS, and the oxidative stress index OSI was calculated. Results: 2-months of ADF treatment reduced body weight BW compared compliance control groups p0.001. All groups cumulative food intake and retroperitoneal fat weight decreased with ADF p0.05. Both 1-month and 2-month ADF interventions had positive effects on reducing TOS and OSI in both liver and serum, with a significant decrease observed in both groups compared to their respective controls p0.001. The liver TAS significantly increased in female rats p0.05, but this increase did not reach a significant level in male rats. The difference in the serum TAS between the groups was not significant. Conclusions: This study evaluated the effects of ADF on BW, food consumption, and oxidative stress parameters in male and female rats. The findings highlight ADFs potential benefits in weight management and reducing oxidative stress. This study represents an important step in understanding the effects of ADF on metabolic health and in identifying potential clinical applications.

 Amaç: Bu çalışmada, gün aşırı açlık protokolünün ADF cinsiyete özgü etkisinin ve yaşlanma ile ilişkili oksidatif stres değişimlerindeki işlevlerinin değerlendirilmesi amaçlandı. Gereç ve yöntem: Kırk iki dişi n21 ve erkek n21 16 aylık Wistar sıçanları altı gruba n7 ayrıldı: Grup-1 kontrol-erkek, Grup-2 1 ay, ADF-erkek, Grup-3 2 ay, ADF-erkek, Grup-4 kontrol-dişi, Grup-5 1 ay, ADFdişi ve Grup-6 2 ay, ADF-dişi. ADF protokolü günaşırı 24 saatlik oruç tutma şeklinde uygulandı haftada üç gün. Serum örnekleri, ELISA yöntemiyle toplam oksidan-antioksidan seviyelerini TOS-TAS ölçmek için alındı ve oksidatif stres indeksi OSI hesaplandı. Bulgular: İki aylık-ADF tedavisinin kümültatif kontrol gruplarıyla karşılaştırıldığında vücut ağırlığında VA anlamlı azalma tespit edildi p0,001. Tüm gruplarda kümülatif gıda alımının ve retroperitoneal yağ ağırlığının ADF ile azaldığı görüldü p0,05. Hem 1 aylık hem de 2 aylık ADF uygulanması karaciğer ve serumda TOS seviyesi ve OSIyi azaltmada olumlu etkiler gösterdi ve her iki grup da kendi kontrollerine göre anlamlı bir azalma gözlemlendi p0,001. Karaciğer TAS seviyesi dişi sıçanlarda anlamlı olarak arttı p0,05, ancak erkek sıçanlarda bu artış anlamlı bir seviyeye ulaşmadı. Gruplar arasındaki serum TAS seviyesinde anlamlı fark saptanmadı. Sonuç: Bu çalışma, erkek ve dişi sıçanlarda ADFnin VA, gıda tüketimi ve oksidatif stres parametreleri üzerindeki etkilerini değerlendirdi. Bulgular, ADFnin kilo yönetimi ve oksidatif stresi azaltmada potansiyel faydalarını vurgulamaktadır. Sonuç olarak, ADFnin metabolik sağlık üzerindeki etkilerini anlamada ve olası klinik uygulamaları belirlemede önemli bir adımı temsil etmektedir.

Article Details

Gender-specific effects of alternate-day fasting on body weight, oxidative stress, and metabolic health in middle-aged rats

Authors:

Özgen Kılıç Erkek | Gülşah Gündoğdu

Publisher:

Pamukkale University

Main Topics:

Tıbbi Fizyoloji (Diğer)

Journal:

Pamukkale Tıp Dergisi Vol.17 Issue 4, pp.690-701

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001315060

pISSN:

1308-0865

Aging

alternate-day fasting

food intake

gender

oxidative stressYaşlanma

alternatif günlerde açlık

gıda alımı

cinsiyet

oksidatif stres

Issues

Article Details

Authors: Özgen Kılıç Erkek | Gülşah Gündoğdu

Publisher: Pamukkale University

Main Topics: Tıbbi Fizyoloji (Diğer)

Journal: Pamukkale Tıp Dergisi Vol.17 Issue 4, pp.690-701

Document Type: Article

Publication Date: 9/2024

Document Language: English

Document ID (Org No.): 2001315060

pISSN: 1308-0865

Issues

Authors:

Özgen Kılıç Erkek | Gülşah Gündoğdu

Publisher:

Pamukkale University

Main Topics:

Tıbbi Fizyoloji (Diğer)

Journal:

Pamukkale Tıp Dergisi Vol.17 Issue 4, pp.690-701

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001315060

pISSN:

1308-0865