Biotyping of Staphylococcus Strains Isolated from Various Sources and Investigations on the Methicillin Resistance

Selda UYSAL; Şükrü KIRKAN

Article Details

Biotyping of Staphylococcus Strains Isolated from Various Sources and Investigations on the Methicillin Resistance

Authors:

Selda UYSAL | Şükrü KIRKAN

Publisher:

Aydin Adnan Menderes University

Journal:

Animal Health Production and Hygiene 2012 Vol.1 Issue 1, pp.11-19

Document Type:

Article

Publication Date:

12/2011

Document Language:

English

Document ID (Org No.):

2000845203

Frequency:

Bi-Annual

eISSN:

2687-5330

pISSN:

2146-7269

Keywords:

Abstract:

 

In this study,  74 Staphylococcus strains were isolated from 120 milk samples obtained from dairy cows with clinical and subclinical mastitis, 42 Staphylococcus strains were isolated from 60 wounds and 7 Staphylococcus strains were isolated from 20 urine samples. Biyotyping was carried out for all Staphylococcus strains. Slime values  +, ++, +++, and -   of coagulase positive Staphylococcus strains were determined as 15%, 12%, 32.8%,  and 40.2%, respectively and coagulase negative strains were determined as 10.7%, 30.4%, 16.0%, and 42.9%, respectively. 41  33.3%  Staphylococcus strains were found to be resistant to methicillin. S. aureus strains  46% , S. intermedius  strains  37.5% , S. haemolyticus strains  43%   and  all S. cohnii subsp cohnii strains  100%  presented similar resistance to vankomisin and teikoplanin.  Of 48% to all of strains were resistant to penicilin and 85% of all strains were found to be  sensitive to eritromisin, 83%  of all strains were found to be  sensitive to sulbactam + ampicillin, and 79% of all strains were found to be  sensitive to sulphamethaxazole + trimethoprim.

 

Bu araştırmada rutin teşhis laboratuarına getirilen klinik ve subklinik mastitisli ineklere ait 120 adet süt örneğinden 74 adet, 60 adet yara yeri svabı örneğinden 42 adet ve 20 adet idrar örneğinden ise 7 adet stafilokok suşu izole edildi. İzole edilen suşların identifikasyonları gerçekleştirilerek Stafilokok suşlarının tiplendirilmeleri yapıldı. Araştırmada koagulaz pozitif Stafilokok suşlarının slime değerleri  +, ++, +++, -  sırasıyla % 15, % 12, % 32.8, % 40.2; koagulaz negatif Stafilokok suşlarının ise slime değerleri  +, ++, +++, -   sırasıyla % 10.7, % 30.4, % 16.0, % 42.9 olarak belirlendi. Çalışmada tiplendirilmeleri gerçekleştirilen Stafilokok suşlarının 41  % 33.3 ’i metisiline dirençli bulundu. S. aureus suşları  % 46 , S. intermedius suşları  % 37.5 , S. haemolyticus suşları  % 43  ve S. cohnii subsp. cohnii suşlarının tamamı  %100  vankomisin ve teikoplanin’e aynı oranlarda dirençlilik göstermişlerdir. Tüm izolatların % 48’inin penisilin’e dirençli ve % 85 oranında eritromisine, % 83 oranında sulbactam + ampisilin’e ve % 79 oranında sulphamethaxsazole + trimethoprim’e duyarlı olduğu saptanmıştır.

Article Details

Biotyping of Staphylococcus Strains Isolated from Various Sources and Investigations on the Methicillin Resistance

Authors:

Selda UYSAL | Şükrü KIRKAN

Publisher:

Aydin Adnan Menderes University

Journal:

Animal Health Production and Hygiene 2012 Vol.1 Issue 1, pp.11-19

Document Type:

Article

Publication Date:

12/2011

Document Language:

English

Document ID (Org No.):

2000845203

Frequency:

Bi-Annual

eISSN:

2687-5330

pISSN:

2146-7269

Staphylococcus sp.

İdentifikasyon

Methisilin Direnci

Antibiyotik Duyarlılık Testi

Issues

Article Details

Biotyping of Staphylococcus Strains Isolated from Various Sources and Investigations on the Methicillin Resistance

Authors: Selda UYSAL | Şükrü KIRKAN

Publisher: Aydin Adnan Menderes University

Journal: Animal Health Production and Hygiene 2012 Vol.1 Issue 1, pp.11-19

Document Type: Article

Publication Date: 12/2011

Document Language: English

Document ID (Org No.): 2000845203

Frequency: Bi-Annual

eISSN: 2687-5330

pISSN: 2146-7269

Staphylococcus sp.

İdentifikasyon

Methisilin Direnci

Antibiyotik Duyarlılık Testi

Issues

Authors:

Selda UYSAL | Şükrü KIRKAN

Publisher:

Aydin Adnan Menderes University

Journal:

Animal Health Production and Hygiene 2012 Vol.1 Issue 1, pp.11-19

Document Type:

Article

Publication Date:

12/2011

Document Language:

English

Document ID (Org No.):

2000845203

Frequency:

Bi-Annual

eISSN:

2687-5330

pISSN:

2146-7269