Features of the Bioconversion of Pentacyclic Triterpenoid Oleanolic Acid Using Rhodococcus Actinobacteria

Natalia A. Luchnikova; Polina Yu. Maltseva; Victoria V. Grishko; Irina B. Ivshina

Article Details

Features of the Bioconversion of Pentacyclic Triterpenoid Oleanolic Acid Using Rhodococcus Actinobacteria

Authors:

Natalia A. Luchnikova | Polina Yu. Maltseva | Victoria V. Grishko | Irina B. Ivshina

Publisher:

KnE Social Sciences

Journal:

KnE Life Sciences Vol.7 Issue 1, pp.304-318

Document Type:

Article

Publication Date:

1/2022

Document Language:

English

Document ID (Org No.):

2001527574

pISSN:

2413-0877

Keywords:

Abstract:

 The ability of actinobacteria of the genus Rhodococcus to transform oleanolic acid (OA), a plant pentacyclic triterpenoid, was shown for the first time using bioresources of the Regional Specialized Collection of AlkanotrophicMicroorganisms (IEGM; WDCM #768;www.iegmcol.ru). The most promising strains (R.opacus IEGM 488 and R.rhodochrousIEGM 285) were selected, and these catalyzed80% bioconversion of OA (0.5 g/L) in the presence of n-hexadecane (0.1% v/v) for seven days. The process of OA bioconversion was accompanied by a gradual decrease in the culture medium pH. Adaptive responses of bacterial cells to the OA effects included the formation of compact cellular aggregates, a marked change in the surface-to-volume ratio of cells, and a significant increase in the Zeta potential values. The results demonstrated that the process of OA bioconversion was catalyzed by membrane-bound enzyme complexes. Participation of cytochrome P450-dependent monooxygenases in the oxidation of the OA moleculewas confirmedusing specific inhibitors. The obtained data expand our knowledge on the catalytic activity of actinobacteria of the genus Rhodococcus and their possible use as biocatalysts for the bioconversion of complex hydrophobic compounds. The results can also be used inthe searchfor promising OA derivatives to be used in the synthesis of biologically active agents.

Article Details

Features of the Bioconversion of Pentacyclic Triterpenoid Oleanolic Acid Using Rhodococcus Actinobacteria

Authors:

Natalia A. Luchnikova | Polina Yu. Maltseva | Victoria V. Grishko | Irina B. Ivshina

Publisher:

KnE Social Sciences

Journal:

KnE Life Sciences Vol.7 Issue 1, pp.304-318

Document Type:

Article

Publication Date:

1/2022

Document Language:

English

Document ID (Org No.):

2001527574

pISSN:

2413-0877

bioconversion

oleanolic acid

Rhodococcus

biologically active compounds

Issues

Article Details

Features of the Bioconversion of Pentacyclic Triterpenoid Oleanolic Acid Using Rhodococcus Actinobacteria

Authors: Natalia A. Luchnikova | Polina Yu. Maltseva | Victoria V. Grishko | Irina B. Ivshina

Publisher: KnE Social Sciences

Journal: KnE Life Sciences Vol.7 Issue 1, pp.304-318

Document Type: Article

Publication Date: 1/2022

Document Language: English

Document ID (Org No.): 2001527574

pISSN: 2413-0877

bioconversion

oleanolic acid

Rhodococcus

biologically active compounds

Issues

Authors:

Natalia A. Luchnikova | Polina Yu. Maltseva | Victoria V. Grishko | Irina B. Ivshina

Publisher:

KnE Social Sciences

Journal:

KnE Life Sciences Vol.7 Issue 1, pp.304-318

Document Type:

Article

Publication Date:

1/2022

Document Language:

English

Document ID (Org No.):

2001527574

pISSN:

2413-0877