Emergence of Vancomycin Resistant Staphylococcus Aureus Isolated from Patients in ICUs of Zagazig University Hospitals

Ghada E. Amr; Sahar Al Gammal

doi:10.12816/0046229

Article Details

Emergence of Vancomycin Resistant Staphylococcus Aureus Isolated from Patients in ICUs of Zagazig University Hospitals

Authors:

Ghada E. Amr | Sahar Al Gammal

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2017 Vol.26 Issue 2, pp.53-59

Document Type:

Article

Publication Date:

4/2017

Document Language:

English

Document ID (Org No.):

116902

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0046229

Descriptors:

Keywords:

Abstract:

 Background: Staphylococcus aureus infections treatment has become more complicatedwith emergence of a methicillin-resistant Staphylococcus aureus (MRSA) strain.Vancomycin has become the drug of choice for the treatment of serious MRSA infectionsSince the 1980s, however its efficacy is now being doubted due to the emergence ofvancomycin resistant staph aureus (VRSA) that are always methicillin-resistant.Objectives: to investigate the emergence of vancomycin resistant Staphylococcus aureusamong Staphylococcus aureus isolates from patients admitted to our hospital ICUs, andto determine the sensitivity of these isolates to different antimicrobial agents also toinvestigate for the presence of vanA gen in VRSA. Methodology: a total of 114Staphylococcus aureus isolates were recovered from different clinical samples ofpatients admitted to ICUs over two years. All isolates were identified by Matrix-assistedlaser desorption ionization–time of flight mass spectrometry and its antimicrobialsusceptibility was investigated by VITEK 2 instrument, MRSA were identified,vancomycin MIC was confirmed by E test, mecA and vanA genes were investigated byPCR technique. Results: among Staphylococcus aureus isolates, there were 90/114(78.9%) diagnosed as MRSA, the most active antimicrobial agent against these MRSAisolates was tigacyclin (100%) followed by linezolid (87.8%). we detected the emergenceof VRSA that were 8.8% (10/114), all VRSA isolates were previously diagnosed asMRSA, percentage of VRSA among MRSA was 11% . The results of PCR showed thatthere were 88/90 MRSA isolates carrying mecA gene and all VRSA (10isolates) werepositive for vanA and mecA genes. Conclusion: The results of this study can beconsidered as an advance warning about the emergence and dissemination of VRSAstrains in our region.

Article Details

Emergence of Vancomycin Resistant Staphylococcus Aureus Isolated from Patients in ICUs of Zagazig University Hospitals

Authors:

Ghada E. Amr | Sahar Al Gammal

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2017 Vol.26 Issue 2, pp.53-59

Document Type:

Article

Publication Date:

4/2017

Document Language:

English

Document ID (Org No.):

116902

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0046229

Descriptors:

STAPHYLOCOCCUS

ANTIBIOTIC RESISTANT BACTERIA

PATIENTS

CRITICAL CARE

HOSPITALS

EGYPT

Emergence

Vancomycin

Staphylococcus Aureus

Issues

Article Details

Authors: Ghada E. Amr | Sahar Al Gammal

Publisher: The Egyptian Society of Medical Microbiology

Main Topics: Medicine

Journal: The Egyptian Journal of Medical Microbiology 2017 Vol.26 Issue 2, pp.53-59

Document Type: Article

Publication Date: 4/2017

Document Language: English

Document ID (Org No.): 116902

Frequency: Quarterly

pISSN: 11102179

DOI: 10.12816/0046229

Descriptors:

Issues

Authors:

Ghada E. Amr | Sahar Al Gammal

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2017 Vol.26 Issue 2, pp.53-59

Document Type:

Article

Publication Date:

4/2017

Document Language:

English

Document ID (Org No.):

116902

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0046229