Investigation of syphilis coinfection and performance of the Architect Syphilis Tp ELISA  screening test in HIV positive patients

Cemile SÖNMEZ; Tülin DEMİR; Figen SEZEN; Selçuk KILIÇ

Article Details

Investigation of syphilis coinfection and performance of the Architect Syphilis Tp ELISA screening test in HIV positive patients

Authors:

Cemile SÖNMEZ | Tülin DEMİR | Figen SEZEN | Selçuk KILIÇ

Publisher:

TUBITAK

Main Topics:

Promotion of health

Journal:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1129-1134

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000750441

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Abstract:

 


 
                         Background/aim:
 Limited data on syphilis coinfection in human immunodeficiency virus (HIV) positive cases exist in Turkey. Our aim 
is to investigate syphilis coinfection and to evaluate the compatibility of the screening Architect Syphilis Tp ELISA with the fluorescent 
treponemal antibody absorption (FTA-abs) confirmation test in HIV positive cases.
Materials  and  methods:
  Totally  519  HIV  positive  patients  were  included  in  the  study.  Enzyme  linked  fluorescent  assay  (ELFA)  was  
used as a screening test and positive samples were confirmed by line immunassay (LIA). In order to discriminate acute HIV infection 
and false ELISA positivity, HIV-1 RNA PCR was performed in ELFA positive and LIA negative samples. Architect Syphilis TP ELISA 
was used for the detection of total antibodies against 
Treponema pallidum
 in HIV positive patients. Positive results were confirmed by 
the FTA-abs test. 
Results:
 Out of 519 HIV-1 positive patients, IgG and IgM positivity, and only IgG positivity was detected as 1.9% and 11.4% in all the 
samples, respectively. A total of 79 (15.2%) sera were positive with Architect Syphilis Tp ELISA test and 69 (13.3%) were positive with 
FTA-abs  test.  Statistically  significant,  almost  perfect  agreement  was  found  between  Architect  Syphilis  Tp  ELISA  and  FTA-abs  tests  
(kappa = 0.921 and P < 0.001). 
Conclusion:
 Implementation of syphilis and HIV screening tests together among risk groups is considered to be appropriate. 
                    

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Article Details

Investigation of syphilis coinfection and performance of the Architect Syphilis Tp ELISA screening test in HIV positive patients

Authors: Cemile SÖNMEZ | Tülin DEMİR | Figen SEZEN | Selçuk KILIÇ

Publisher: TUBITAK

Main Topics: Promotion of health

Journal: Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1129-1134

Document Type: Article

Publication Date: 12/2018

Document Language: English

Document ID (Org No.): 2000750441

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Issues

Authors:

Cemile SÖNMEZ | Tülin DEMİR | Figen SEZEN | Selçuk KILIÇ

Publisher:

TUBITAK

Main Topics:

Promotion of health

Journal:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 6, pp.1129-1134

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000750441

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144