Isolation, identification, and antifungal resistance of Candida species from various samples

Khaled Nasher Qahtan Salem; Abdullah Salem Ben Barakat Bawazir; Asia Saleh Moqbel Muthana; Asrar Mohammed Abdullah Ali; Mariam Ali Haitham Ahmed; Muna Yaser Ahmed Saif; Samah Mokhtar Salem Alhaithami; Wala Fahd Hashem Mohammed; Zaina Mohammed Mothna Rashed

Article Details

Isolation, identification, and antifungal resistance of Candida species from various samples

Authors:

Khaled Nasher Qahtan Salem | Abdullah Salem Ben Barakat Bawazir | Asia Saleh Moqbel Muthana | Asrar Mohammed Abdullah Ali | Mariam Ali Haitham Ahmed | Muna Yaser Ahmed Saif | Samah Mokhtar Salem Alhaithami | Wala Fahd Hashem Mohammed | Zaina Mohammed Mothna Rashed

Publisher:

University of Aden

Main Topics:

Antifungal resistance

Journal:

University of Aden Journal of Natural and Applied Sciences Vol.27 Issue 2, pp.233-248

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001535149

pISSN:

2788-9327

Keywords:

Abstract:

 This study in Aden, Yemen, collected 67 samples from patients attending hospitals and laboratories. Samples were taken from different body areas, covering various age groups and genders. Fungal infections, particularly Candida species, are a significant cause of death worldwide. The emergence of azole-resistant Candida has raised concerns and highlighted the need for better antifungal agents.In the study, 68.66% of the collected samples contained pathogenic Candida fungi. Some samples also showed bacterial growth or both bacteria and fungi. Suspected Candida growth on corn meal agar exhibited chlamydospore formation and germ tubes on human serum. Carbohydrate fermentation tests were positive for Glucose, Maltose, Galactose, and Xylose, while Sucrose and Lactose were negative. All Candida isolates (100%) were accurately identified to the species level using CHROM agar Candida. Candida's pathogenicity was linked to various virulence factors, including hydrolytic enzyme secretion. Nystatin was the most effective antifungal agent (100% sensitivity), while Fluconazole (73.92%) and Amphotericin B (52.17%) showed varying levels of sensitivity. However, Voriconazole, ketoconazole, and Miconazole displayed high resistance rates (89.13%, 84.78%, and 80.44%, respectively) against the isolated Candida.This study concluded that Candida spp., particularly Candida albicans, were frequently found in the samples from patients, with vaginal swaps being a common site of infection. The identification of Candida isolates to the species level using CHROM agar Candida was accurate. Nystatin, Fluconazole, and Amphotericin B were the most effective antifungal agents against Candida spp.

Article Details

Isolation, identification, and antifungal resistance of Candida species from various samples

Authors:

Khaled Nasher Qahtan Salem | Abdullah Salem Ben Barakat Bawazir | Asia Saleh Moqbel Muthana | Asrar Mohammed Abdullah Ali | Mariam Ali Haitham Ahmed | Muna Yaser Ahmed Saif | Samah Mokhtar Salem Alhaithami | Wala Fahd Hashem Mohammed | Zaina Mohammed Mothna Rashed

Publisher:

University of Aden

Main Topics:

Antifungal resistance

Journal:

University of Aden Journal of Natural and Applied Sciences Vol.27 Issue 2, pp.233-248

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001535149

pISSN:

2788-9327

: Candida species

Candidiasis

Antifungal resistance

Issues

Article Details

Isolation, identification, and antifungal resistance of Candida species from various samples

Authors: Khaled Nasher Qahtan Salem | Abdullah Salem Ben Barakat Bawazir | Asia Saleh Moqbel Muthana | Asrar Mohammed Abdullah Ali | Mariam Ali Haitham Ahmed | Muna Yaser Ahmed Saif | Samah Mokhtar Salem Alhaithami | Wala Fahd Hashem Mohammed | Zaina Mohammed Mothna Rashed

Publisher: University of Aden

Main Topics: Antifungal resistance

Journal: University of Aden Journal of Natural and Applied Sciences Vol.27 Issue 2, pp.233-248

Document Type: Article

Publication Date: 10/2023

Document Language: English

Document ID (Org No.): 2001535149

pISSN: 2788-9327

: Candida species

Candidiasis

Antifungal resistance

Issues

Authors:

Khaled Nasher Qahtan Salem | Abdullah Salem Ben Barakat Bawazir | Asia Saleh Moqbel Muthana | Asrar Mohammed Abdullah Ali | Mariam Ali Haitham Ahmed | Muna Yaser Ahmed Saif | Samah Mokhtar Salem Alhaithami | Wala Fahd Hashem Mohammed | Zaina Mohammed Mothna Rashed

Publisher:

University of Aden

Main Topics:

Antifungal resistance

Journal:

University of Aden Journal of Natural and Applied Sciences Vol.27 Issue 2, pp.233-248

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001535149

pISSN:

2788-9327