Effect of High Cholesterol Diet and α-Tocopherol Supplementation on Endoplasmic Retüculum Stress and Apoptosis in Hippocampus Tissue

Erdi SOZEN; Tugce DEMIREL-YALCINER; Aslı ECE; Alper ISMICOGLU; Nesrin KARTAL ÖZER

doi:https://doi.org/10.33808/clinexphealthsci.972222

Article Details

Effect of High Cholesterol Diet and α-Tocopherol Supplementation on Endoplasmic Retüculum Stress and Apoptosis in Hippocampus Tissue

Authors:

Erdi SOZEN | Tugce DEMIREL-YALCINER | Aslı ECE | Alper ISMICOGLU | Nesrin KARTAL ÖZER

Publisher:

Marmara University

Main Topics:

Promotion of health

Journal:

Clinical and Experimental Health Sciences 2022 Vol.12 Issue 2, pp.439-444

Document Type:

Article

Publication Date:

6/2022

Document Language:

English

Document ID (Org No.):

2000906555

Frequency:

Monthly

eISSN:

2459-1459

DOI:

https://doi.org/10.33808/clinexphealthsci.972222

Abstract:

 

Objective: Neurodegenerative diseases are life-threatening disorders that occur when cells in peripheral nervous system or brain lose function over time and ultimately die. Our study aimed to establish a high cholesterol fed rabbit model to investigate the status of endoplasmic reticulum (ER) stress, proteasome activity and apoptosis as well as the beneficial role of α-tocopherol on hippocampus.

Methods: Hypercholesterolemic rabbit model was established by feeding on 2% cholesterol diet for 8 weeks, and α-tocopherol supplementation was applied with intramuscular injections. Alterations of Grp78, Grp94, Bax, Bcl-2 and BACE1 levels in hippocampus tissue were determined by western blotting, while proteasome activity was measured by fluorometric detection.

Results: Cholesterol containing diet for 8 weeks increased well-known parameters of ER stress and apoptosis, together with no chance in proteasome activity. On the other hand, α-tocopherol supplementation in rabbits fed two percent cholesterol containing diet significantly down-regulated certain ER stress and apoptosis parameters along with an increase in proteasome activity.
Conclusion: Our results underline a novel function of α-tocopherol in reducing cholesterol induced ER stress and apoptosis by increasing proteasome activity in the hippocampus.

Article Details

Effect of High Cholesterol Diet and α-Tocopherol Supplementation on Endoplasmic Retüculum Stress and Apoptosis in Hippocampus Tissue

Authors: Erdi SOZEN | Tugce DEMIREL-YALCINER | Aslı ECE | Alper ISMICOGLU | Nesrin KARTAL ÖZER

Publisher: Marmara University

Main Topics: Promotion of health

Journal: Clinical and Experimental Health Sciences 2022 Vol.12 Issue 2, pp.439-444

Document Type: Article

Publication Date: 6/2022

Document Language: English

Document ID (Org No.): 2000906555

Frequency: Monthly

eISSN: 2459-1459

DOI: https://doi.org/10.33808/clinexphealthsci.972222

Issues

Authors:

Erdi SOZEN | Tugce DEMIREL-YALCINER | Aslı ECE | Alper ISMICOGLU | Nesrin KARTAL ÖZER

Publisher:

Marmara University

Main Topics:

Promotion of health

Journal:

Clinical and Experimental Health Sciences 2022 Vol.12 Issue 2, pp.439-444

Document Type:

Article

Publication Date:

6/2022

Document Language:

English

Document ID (Org No.):

2000906555

Frequency:

Monthly

eISSN:

2459-1459

DOI:

https://doi.org/10.33808/clinexphealthsci.972222