Effect of growth regulators on tissue culture parameters in rice (Oryza sativa L.)

Berk BENLİOĞLU; Duygu Ege TUNA; Melahat Avcı BİRSİN; Ahmet Murat OZGEN

Article Details

Effect of growth regulators on tissue culture parameters in rice (Oryza sativa L.)

Authors:

Berk BENLİOĞLU | Duygu Ege TUNA | Melahat Avcı BİRSİN | Ahmet Murat OZGEN

Publisher:

Bitki Islahçıları Alt Birliği

Journal:

Ekin Journal of Crop Breeding and Genetics 2015 Vol.1 Issue 2, pp.43-46

Document Type:

Article

Publication Date:

6/2015

Document Language:

Turkish

Document ID (Org No.):

2000403735

eISSN:

2459-069X

pISSN:

2149-1275

Abstract:

 

This study was conducted in Ankara University, Faculty of Agriculture, Department of Field crops, Biotechnology Laboratory. The objective of the present study was to determine the effects of growth regulators on tissue parameters in rice. In this study, mature embryos of three rice cultivars (Aromatik-1, Baldo and Karadeniz) and different growth regulators (2,4-Dichlorophenoxyacetic acid (2,4-D) and picloram) were used as material. For callus induction, mature embryos were placed with scutellum upwards on three different medium (hormone-free MS-0, MS + 2 mg/l 2,4-D and MS + 2.5 mg/l picloram) in sterile Petri dishes for two weeks at 25±10C in darkness. After incubation; obtained calli were transferred to hormone-free MS-0 medium for regeneration. According to results, the effect of growth regulators and genotypes on callus induction and plant regeneration in rice were found to be statistically significant.

Article Details

Effect of growth regulators on tissue culture parameters in rice (Oryza sativa L.)

Authors: Berk BENLİOĞLU | Duygu Ege TUNA | Melahat Avcı BİRSİN | Ahmet Murat OZGEN

Publisher: Bitki Islahçıları Alt Birliği

Journal: Ekin Journal of Crop Breeding and Genetics 2015 Vol.1 Issue 2, pp.43-46

Document Type: Article

Publication Date: 6/2015

Document Language: Turkish

Document ID (Org No.): 2000403735

eISSN: 2459-069X

pISSN: 2149-1275

Issues

Authors:

Berk BENLİOĞLU | Duygu Ege TUNA | Melahat Avcı BİRSİN | Ahmet Murat OZGEN

Publisher:

Bitki Islahçıları Alt Birliği

Journal:

Ekin Journal of Crop Breeding and Genetics 2015 Vol.1 Issue 2, pp.43-46

Document Type:

Article

Publication Date:

6/2015

Document Language:

Turkish

Document ID (Org No.):

2000403735

eISSN:

2459-069X

pISSN:

2149-1275