Optimization of nanosilver purification process with Camellia sinensis L. extract as bioreductor

Florentinus Dika Octa Riswanto

Article Details

Optimization of nanosilver purification process with Camellia sinensis L. extract as bioreductor

Authors:

Florentinus Dika Octa Riswanto

Publisher:

Marmara University

Main Topics:

Pharmaceutical Delivery Technologies

Journal:

Journal of Research in Pharmacy Vol.29 Issue 5, pp.1950-1958

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001342409

pISSN:

2630-6344

Keywords:

Abstract:

 Nanosilver can be described as a nanoparticle synthesized from silver metal with a size range of 1100 nm. Nanosilver synthesis can be performed using green tea leaf extract Camellia sinensis L. as a bioreductor due to its flavonoid content. The content of flavonoid compounds is able to reduce silver metal ions Ag derived from AgNO3 as metal precursors so that silver nanoparticles can be produced. This study aimed to obtain the optimum time and speed of centrifugation in nanosilver purification with green tea leaf bioreductors using the Central Composite Design CCD method. This study used two independent variables namely the duration and speed of centrifugation. The effect of centrifugation duration and speed on wavelength, transmittance percentage, and particle size of purified were analyzed by response surface methodology using the R software. The duration and speed of the optimum formula for the nanosilver purification process were obtained using the CCD method. The optimum conditions obtained were the centrifugation duration of 22 minutes and the centrifugation speed of 3500 rpm. Furthermore, the content of tannin compounds in green tea leaves was determined using thin layer chromatography.

 Nanosilver can be described as a nanoparticle synthesized from silver metal with a size range of 1100 nm. Nanosilver synthesis can be performed using green tea leaf extract Camellia sinensis L. as a bioreductor due to its flavonoid content. The content of flavonoid compounds is able to reduce silver metal ions Ag derived from AgNO3 as metal precursors so that silver nanoparticles can be produced. This study aimed to obtain the optimum time and speed of centrifugation in nanosilver purification with green tea leaf bioreductors using the Central Composite Design CCD method. This study used two independent variables namely the duration and speed of centrifugation. The effect of centrifugation duration and speed on wavelength, transmittance percentage, and particle size of purified were analyzed by response surface methodology using the R software. The duration and speed of the optimum formula for the nanosilver purification process were obtained using the CCD method. The optimum conditions obtained were the centrifugation duration of 22 minutes and the centrifugation speed of 3500 rpm. Furthermore, the content of tannin compounds in green tea leaves was determined using thin layer chromatography.

 Nanosilver can be described as a nanoparticle synthesized from silver metal with a size range of 1100 nm. Nanosilver synthesis can be performed using green tea leaf extract Camellia sinensis L. as a bioreductor due to its flavonoid content. The content of flavonoid compounds is able to reduce silver metal ions Ag derived from AgNO3 as metal precursors so that silver nanoparticles can be produced. This study aimed to obtain the optimum time and speed of centrifugation in nanosilver purification with green tea leaf bioreductors using the Central Composite Design CCD method. This study used two independent variables namely the duration and speed of centrifugation. The effect of centrifugation duration and speed on wavelength, transmittance percentage, and particle size of purified were analyzed by response surface methodology using the R software. The duration and speed of the optimum formula for the nanosilver purification process were obtained using the CCD method. The optimum conditions obtained were the centrifugation duration of 22 minutes and the centrifugation speed of 3500 rpm. Furthermore, the content of tannin compounds in green tea leaves was determined using thin layer chromatography.

 Nanosilver can be described as a nanoparticle synthesized from silver metal with a size range of 1100 nm. Nanosilver synthesis can be performed using green tea leaf extract Camellia sinensis L. as a bioreductor due to its flavonoid content. The content of flavonoid compounds is able to reduce silver metal ions Ag derived from AgNO3 as metal precursors so that silver nanoparticles can be produced. This study aimed to obtain the optimum time and speed of centrifugation in nanosilver purification with green tea leaf bioreductors using the Central Composite Design CCD method. This study used two independent variables namely the duration and speed of centrifugation. The effect of centrifugation duration and speed on wavelength, transmittance percentage, and particle size of purified were analyzed by response surface methodology using the R software. The duration and speed of the optimum formula for the nanosilver purification process were obtained using the CCD method. The optimum conditions obtained were the centrifugation duration of 22 minutes and the centrifugation speed of 3500 rpm. Furthermore, the content of tannin compounds in green tea leaves was determined using thin layer chromatography.

Article Details

Optimization of nanosilver purification process with Camellia sinensis L. extract as bioreductor

Authors:

Florentinus Dika Octa Riswanto

Publisher:

Marmara University

Main Topics:

Pharmaceutical Delivery Technologies

Journal:

Journal of Research in Pharmacy Vol.29 Issue 5, pp.1950-1958

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001342409

pISSN:

2630-6344

Nanosilver

green tea leaves

CCD

sonication

purification

tannin

Issues

Article Details

Optimization of nanosilver purification process with Camellia sinensis L. extract as bioreductor

Authors: Florentinus Dika Octa Riswanto

Publisher: Marmara University

Main Topics: Pharmaceutical Delivery Technologies

Journal: Journal of Research in Pharmacy Vol.29 Issue 5, pp.1950-1958

Document Type: Article

Publication Date: 1/2025

Document Language: English

Document ID (Org No.): 2001342409

pISSN: 2630-6344

Nanosilver

green tea leaves

CCD

sonication

purification

tannin

Issues

Authors:

Florentinus Dika Octa Riswanto

Publisher:

Marmara University

Main Topics:

Pharmaceutical Delivery Technologies

Journal:

Journal of Research in Pharmacy Vol.29 Issue 5, pp.1950-1958

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001342409

pISSN:

2630-6344