Effect of caffeine supplementation during treadmill exercise on hippocampal genes expression levels in adolescent rats

Fatma Bakır; Özge Selin Çevik; Seval Müsüroğlu Keloğlan; Leyla Şahin

Article Details

Effect of caffeine supplementation during treadmill exercise on hippocampal genes expression levels in adolescent rats

Authors:

Fatma Bakır | Özge Selin Çevik | Seval Müsüroğlu Keloğlan | Leyla Şahin

Publisher:

Prusa Medical Publishing

Main Topics:

Clinical Sciences

Journal:

The European Research Journal Vol.9 Issue 2, pp.348-358

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001283016

pISSN:

2149-3189

Keywords:

Abstract:

 Objectives: The usage of caffeine, a psychostimulant that is included in many foods and drinks, is rising, especially among young people. Also, caffeine works as an ergogenic substance during exercise to improve performance, and strength. In this study, our aim was to investigate the effect of caffeine consumption and exercise on hippocampal learning and memory functions in early life. Methods: Postnatal 28 days old Wistar albino male rats n  28 were randomly divided into 4 groups; control group C, caffeine group Cf, exercise group E, caffeineexercise group CfE. Caffeine was dissolved in drinking water 0.3 gL and the treadmill exercise was applied 3 days a week. Following, the rats were applied to Morris Water Maze Test MWMT and open field test. N-methyl-D-aspartate NMDA receptors NR2A, NR2B, and brain-derived neurotrophic factor BDNF gene expression levels were investigated in hippocampus tissue by RT-PCR. Results: In MWMT, there was no significant difference in terms of learning and memory functions and hippocampal gene expression levels of the groups  p  0.05. In the open field test, the time spent in the center was decreased in the CfE group, and the number of entries to the center was decreased in the E and CfE groups compared to the control group  p  0.05. Conclusions: We assumed that caffeine given with exercise application caused anxiety behavior but did not affect learning and memory. There is a need for new studies investigating the effect of caffeine on exercise with different doses and durations depending on age.

 Objectives: The usage of caffeine, a psychostimulant that is included in many foods and drinks, is rising, especially among young people. Also, caffeine works as an ergogenic substance during exercise to improve performance, and strength. In this study, our aim was to investigate the effect of caffeine consumption and exercise on hippocampal learning and memory functions in early life. Methods: Postnatal 28 days old Wistar albino male rats n  28 were randomly divided into 4 groups; control group C, caffeine group Cf, exercise group E, caffeineexercise group CfE. Caffeine was dissolved in drinking water 0.3 gL and the treadmill exercise was applied 3 days a week. Following, the rats were applied to Morris Water Maze Test MWMT and open field test. N-methyl-D-aspartate NMDA receptors NR2A, NR2B, and brain-derived neurotrophic factor BDNF gene expression levels were investigated in hippocampus tissue by RT-PCR. Results: In MWMT, there was no significant difference in terms of learning and memory functions and hippocampal gene expression levels of the groups  p  0.05. In the open field test, the time spent in the center was decreased in the CfE group, and the number of entries to the center was decreased in the E and CfE groups compared to the control group  p  0.05. Conclusions: We assumed that caffeine given with exercise application caused anxiety behavior but did not affect learning and memory. There is a need for new studies investigating the effect of caffeine on exercise with different doses and durations depending on age.

 Objectives: The usage of caffeine, a psychostimulant that is included in many foods and drinks, is rising, especially among young people. Also, caffeine works as an ergogenic substance during exercise to improve performance, and strength. In this study, our aim was to investigate the effect of caffeine consumption and exercise on hippocampal learning and memory functions in early life. Methods: Postnatal 28 days old Wistar albino male rats n  28 were randomly divided into 4 groups; control group C, caffeine group Cf, exercise group E, caffeineexercise group CfE. Caffeine was dissolved in drinking water 0.3 gL and the treadmill exercise was applied 3 days a week. Following, the rats were applied to Morris Water Maze Test MWMT and open field test. N-methyl-D-aspartate NMDA receptors NR2A, NR2B, and brain-derived neurotrophic factor BDNF gene expression levels were investigated in hippocampus tissue by RT-PCR. Results: In MWMT, there was no significant difference in terms of learning and memory functions and hippocampal gene expression levels of the groups  p  0.05. In the open field test, the time spent in the center was decreased in the CfE group, and the number of entries to the center was decreased in the E and CfE groups compared to the control group  p  0.05. Conclusions: We assumed that caffeine given with exercise application caused anxiety behavior but did not affect learning and memory. There is a need for new studies investigating the effect of caffeine on exercise with different doses and durations depending on age.

 Objectives: The usage of caffeine, a psychostimulant that is included in many foods and drinks, is rising, especially among young people. Also, caffeine works as an ergogenic substance during exercise to improve performance, and strength. In this study, our aim was to investigate the effect of caffeine consumption and exercise on hippocampal learning and memory functions in early life. Methods: Postnatal 28 days old Wistar albino male rats n  28 were randomly divided into 4 groups; control group C, caffeine group Cf, exercise group E, caffeineexercise group CfE. Caffeine was dissolved in drinking water 0.3 gL and the treadmill exercise was applied 3 days a week. Following, the rats were applied to Morris Water Maze Test MWMT and open field test. N-methyl-D-aspartate NMDA receptors NR2A, NR2B, and brain-derived neurotrophic factor BDNF gene expression levels were investigated in hippocampus tissue by RT-PCR. Results: In MWMT, there was no significant difference in terms of learning and memory functions and hippocampal gene expression levels of the groups  p  0.05. In the open field test, the time spent in the center was decreased in the CfE group, and the number of entries to the center was decreased in the E and CfE groups compared to the control group  p  0.05. Conclusions: We assumed that caffeine given with exercise application caused anxiety behavior but did not affect learning and memory. There is a need for new studies investigating the effect of caffeine on exercise with different doses and durations depending on age.

Article Details

Effect of caffeine supplementation during treadmill exercise on hippocampal genes expression levels in adolescent rats

Authors: Fatma Bakır | Özge Selin Çevik | Seval Müsüroğlu Keloğlan | Leyla Şahin

Publisher: Prusa Medical Publishing

Main Topics: Clinical Sciences

Journal: The European Research Journal Vol.9 Issue 2, pp.348-358

Document Type: Article

Publication Date: 4/2023

Document Language: English

Document ID (Org No.): 2001283016

pISSN: 2149-3189

Caffeine

exercise

hippocampus

learning

memory

Issues

Authors:

Fatma Bakır | Özge Selin Çevik | Seval Müsüroğlu Keloğlan | Leyla Şahin

Publisher:

Prusa Medical Publishing

Main Topics:

Clinical Sciences

Journal:

The European Research Journal Vol.9 Issue 2, pp.348-358

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001283016

pISSN:

2149-3189