Voltammetric, UV Absorption and Viscometric Studies of the Interaction of Norepinephrine with DNA

Mehmet ASLANOĞLU; Necla ÖGE

Article Details

Voltammetric, UV Absorption and Viscometric Studies of the Interaction of Norepinephrine with DNA

Authors:

Mehmet ASLANOĞLU | Necla ÖGE

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2005 Vol.29 Issue 5, pp.477-485

Document Type:

Article

Publication Date:

4/2005

Document Language:

Turkish

Document ID (Org No.):

2000729027

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527

Abstract:

 

Voltammetric, UV-vis spectroscopic and viscometric methods  were used to investigate the interaction between norepinephrine and  DNA. The results of square wave voltammetric and UV-absorption  measurements have shown that norepinephrine was bound to DNA. Besides  the electrostatic interaction between DNA and norepinephrine, hydrogen  bonds and intercalation may also exist.  Viscosity measurements also  suggest that NE is bound to DNA via an electrostatic mode of  interaction at lower norepinephrine concentrations.  However, at  higher concentrations of norpinephrine, the increase in the relative  viscosity of DNA indicated that the intercalation is also involved in  the binding.  The binding constant and the binding site size for the  interaction of norepinephrine with DNA were K = 3.3 (\pm 0.18) \times  103M-1, s = 2.89 (\pm 0.05) base pairs and K = 5.15 (\pm 0.38)  \times 103M-1, s = 2.41 (\pm 0.04) base pairs for the limiting  conditions of static and mobile binding equilibrium, respectively.   The binding site size determined shows that norepinephrine covers more  than 2 base pairs upon binding to DNA. The standard Gibbs free energy  change (D G0=-RT ln K) comes out to be approximately -21.17  kJ/mol at 25 °C, which indicates the spontaneity of the binding of NE  with DNA.

 

Voltammetric, UV-vis spectroscopic and viscometric methods  were used to investigate the interaction between norepinephrine and  DNA. The results of square wave voltammetric and UV-absorption  measurements have shown that norepinephrine was bound to DNA. Besides  the electrostatic interaction between DNA and norepinephrine, hydrogen  bonds and intercalation may also exist.  Viscosity measurements also  suggest that NE is bound to DNA via an electrostatic mode of  interaction at lower norepinephrine concentrations.  However, at  higher concentrations of norpinephrine, the increase in the relative  viscosity of DNA indicated that the intercalation is also involved in  the binding.  The binding constant and the binding site size for the  interaction of norepinephrine with DNA were K = 3.3 (\pm 0.18) \times  103M-1, s = 2.89 (\pm 0.05) base pairs and K = 5.15 (\pm 0.38)  \times 103M-1, s = 2.41 (\pm 0.04) base pairs for the limiting  conditions of static and mobile binding equilibrium, respectively.   The binding site size determined shows that norepinephrine covers more  than 2 base pairs upon binding to DNA. The standard Gibbs free energy  change (D G0=-RT ln K) comes out to be approximately -21.17  kJ/mol at 25 °C, which indicates the spontaneity of the binding of NE  with DNA.

Article Details

Voltammetric, UV Absorption and Viscometric Studies of the Interaction of Norepinephrine with DNA

Authors: Mehmet ASLANOĞLU | Necla ÖGE

Publisher: TUBITAK

Journal: Turkish Journal of Chemistry 2005 Vol.29 Issue 5, pp.477-485

Document Type: Article

Publication Date: 4/2005

Document Language: Turkish

Document ID (Org No.): 2000729027

Frequency: Bimonthly

eISSN: 1303-6130

pISSN: 1300-0527

Issues

Authors:

Mehmet ASLANOĞLU | Necla ÖGE

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2005 Vol.29 Issue 5, pp.477-485

Document Type:

Article

Publication Date:

4/2005

Document Language:

Turkish

Document ID (Org No.):

2000729027

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527