Biodegradación de Hidrocarburos en Sedimento de la Bahía de Manzanillo

Ariel Antonio Grey Garibaldi

Article Details

Biodegradación de Hidrocarburos en Sedimento de la Bahía de Manzanillo

Authors:

Ariel Antonio Grey Garibaldi

Publisher:

KnE Social Sciences

Journal:

KnE Engineering Vol.3 Issue 1, pp.80-87

Document Type:

Article

Publication Date:

11/2018

Document Language:

English

Document ID (Org No.):

2001523276

pISSN:

2518-6841

Keywords:

Abstract:

 The capacities of biodegradable were evaluated, isolated microbial consortia in the port area near the Panama Canal, resulting from enrichment in different carbon sources. The inoculation was performed with 5 g of sediment in 250 ml Erlenmeyer flasks with 0.055% naphthalene, phenanthrene and pyrene. Growth was determined by the optical density (OD) colorimetric method and the droplet counting method. The identification procedure was performed using biochemical tests API Coryne System (bioMérieux). This study the growth capacity, triplicate concentrations ranging from 125 ppm to 2000 ppm were added and incubated at 28 ° C with constant stirring at 150 rpm. Rhodococcus equi strains were found with greater abundance relative to Corynebacterium propingun (3: 2). According to the Monod Equation, Ks of 458 mg / L and Ks of 403 mg / L were obtained and the μmax obtained were 1.38X107 CFU / mL / h and 1.11X108 CFU / mL / h. It is recommended to analyze the kinetics of biodegradation at concentrations higher than 2000 mg / L in order to find the inhibition constant; Perform bioassays with different combinations of hydrocarbons and compare the kinetics of isolated versus kinetic strains in a consortium.

Article Details

Biodegradación de Hidrocarburos en Sedimento de la Bahía de Manzanillo

Authors:

Ariel Antonio Grey Garibaldi

Publisher:

KnE Social Sciences

Journal:

KnE Engineering Vol.3 Issue 1, pp.80-87

Document Type:

Article

Publication Date:

11/2018

Document Language:

English

Document ID (Org No.):

2001523276

pISSN:

2518-6841

Growth capacity

enrichment

carbon source

insulation

biodegradation kinetics.

Issues

Article Details

Biodegradación de Hidrocarburos en Sedimento de la Bahía de Manzanillo

Authors: Ariel Antonio Grey Garibaldi

Publisher: KnE Social Sciences

Journal: KnE Engineering Vol.3 Issue 1, pp.80-87

Document Type: Article

Publication Date: 11/2018

Document Language: English

Document ID (Org No.): 2001523276

pISSN: 2518-6841

Growth capacity

enrichment

carbon source

insulation

biodegradation kinetics.

Issues

Authors:

Ariel Antonio Grey Garibaldi

Publisher:

KnE Social Sciences

Journal:

KnE Engineering Vol.3 Issue 1, pp.80-87

Document Type:

Article

Publication Date:

11/2018

Document Language:

English

Document ID (Org No.):

2001523276

pISSN:

2518-6841