Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Sine ÖZMEN TOĞAY; Ayhan TEMİZ; Ayten ÇELEBİ; Leyla AÇIK; Sıddika Songül YALÇIN

Article Details

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Authors:

Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000812194

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg2, gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

 

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and some virulence genes (agg2, gelE, efaAfm, ccf, cpd, cad, cylM, cylB, etc.) were investigated using polymerase chain reaction (PCR). All strains were sensitive to ampicillin, penicillin G, chloramphenicol, and vancomycin. None of the E. faecalis isolates contained vanA, vanB, or efaAfm genes. The results of this study indicated that there were no harmful enterococci strains in human milk and colostrum samples in terms of tested virulence factors and antibiotic resistance. Therefore, the E. faecalis isolates from human milk may have the potential to be considered as a functional culture for the food industry.

Article Details

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Authors:

Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000812194

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Enterococcus faecalis

virulence gene

antibiotic resistance

human milk

colostrum

Issues

Article Details

Investigation of potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples

Authors: Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Document Type: Article

Publication Date: 5/2014

Document Language: Turkish

Document ID (Org No.): 2000812194

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Enterococcus faecalis

virulence gene

antibiotic resistance

human milk

colostrum

Issues

Authors:

Sine ÖZMEN TOĞAY | Ayhan TEMİZ | Ayten ÇELEBİ | Leyla AÇIK | Sıddika Songül YALÇIN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2014 Vol.38 Issue 3, pp.357-364

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000812194

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152