Detection of Cucumber Mosaic Virus (CMV) in Cucurbits by TaqMan Real-Time RT-PCR in Hakkari Province, Türkiye

Ayşenur Biltekin; Nevin Akdura

Article Details

Detection of Cucumber Mosaic Virus (CMV) in Cucurbits by TaqMan Real-Time RT-PCR in Hakkari Province, Türkiye

Authors:

Ayşenur Biltekin | Nevin Akdura

Publisher:

Mevlüt AKÇURA

Main Topics:

Phytopathology

Journal:

Turkish Journal of Agricultural and Natural Sciences Vol.11 Issue 3, pp.825-834

Document Type:

Article

Publication Date:

7/2024

Document Language:

English

Document ID (Org No.):

2001323712

pISSN:

2148-3647

Keywords:

Abstract:

 Cucumber mosaic virus CMV is among the important viruses that can infect many crops. In 2022, this study was conducted in the Kırıkdağ, Otluca, and Merzan areas of the Hakkari province of Türkiye to investigate the presence of CMV in cucurbits exhibiting viral disease symptoms yellowing, necrosis, mosaic, and deformity. A total of 50 samples; 7 watermelons Citrullus lanatus, 3 melons Cucumis melo L., 17 cucumbers Cucumis sativus L., 8 zucchinis Cucurbita pepo L., 9 pumpkins Cucurbita moschata, 2 gourds Lagenaria siceraria and 4 gherkins Cucumis melo var. flexuous were collected. The presence of CMV in the samples was investigated by TaqMan real-time RT-PCR using probes and primers specific to the 105 bp region of partial the coat protein gene of the virus. CMV-specific viral RNA was detected in 6 12 of the 50 samples analyzed. CMV was detected most commonly in the Otluca area and melon 33.3 among cucurbit varieties. CMV agent was not detected in the samples collected from the Merzan area. The TaqMan real-time RT-PCR assay used in this study demonstrated that the method can be widely used for the general detection of CMV in a wide range of hosts. This is the first report on the detection of CMV in cucurbits in the Hakkari province of Türkiye.

 Cucumber mosaic virus CMV is among the important viruses that can infect many crops. In 2022, this study was conducted in the Kırıkdağ, Otluca, and Merzan areas of the Hakkari province of Türkiye to investigate the presence of CMV in cucurbits exhibiting viral disease symptoms yellowing, necrosis, mosaic, and deformity. A total of 50 samples; 7 watermelons Citrullus lanatus, 3 melons Cucumis melo L., 17 cucumbers Cucumis sativus L., 8 zucchinis Cucurbita pepo L., 9 pumpkins Cucurbita moschata, 2 gourds Lagenaria siceraria and 4 gherkins Cucumis melo var. flexuous were collected. The presence of CMV in the samples was investigated by TaqMan real-time RT-PCR using probes and primers specific to the 105 bp region of partial the coat protein gene of the virus. CMV-specific viral RNA was detected in 6 12 of the 50 samples analyzed. CMV was detected most commonly in the Otluca area and melon 33.3 among cucurbit varieties. CMV agent was not detected in the samples collected from the Merzan area. The TaqMan real-time RT-PCR assay used in this study demonstrated that the method can be widely used for the general detection of CMV in a wide range of hosts. This is the first report on the detection of CMV in cucurbits in the Hakkari province of Türkiye.

 Cucumber mosaic virus CMV is among the important viruses that can infect many crops. In 2022, this study was conducted in the Kırıkdağ, Otluca, and Merzan areas of the Hakkari province of Türkiye to investigate the presence of CMV in cucurbits exhibiting viral disease symptoms yellowing, necrosis, mosaic, and deformity. A total of 50 samples; 7 watermelons Citrullus lanatus, 3 melons Cucumis melo L., 17 cucumbers Cucumis sativus L., 8 zucchinis Cucurbita pepo L., 9 pumpkins Cucurbita moschata, 2 gourds Lagenaria siceraria and 4 gherkins Cucumis melo var. flexuous were collected. The presence of CMV in the samples was investigated by TaqMan real-time RT-PCR using probes and primers specific to the 105 bp region of partial the coat protein gene of the virus. CMV-specific viral RNA was detected in 6 12 of the 50 samples analyzed. CMV was detected most commonly in the Otluca area and melon 33.3 among cucurbit varieties. CMV agent was not detected in the samples collected from the Merzan area. The TaqMan real-time RT-PCR assay used in this study demonstrated that the method can be widely used for the general detection of CMV in a wide range of hosts. This is the first report on the detection of CMV in cucurbits in the Hakkari province of Türkiye.

 Hıyar mozaik virüsü Cucumber mosaic virus; CMV birçok ürünü enfekte edebilen önemli virüsler arasındadır. Bu çalışma, 2022 yılında Hakkari ili Kırıkdağ, Otluca ve Merzan alanlarında viral hastalık belirtileri sararma, nekroz, mozaik ve şekil bozukluğu gösteren kabakgillerde CMV varlığının araştırılması amacı ile yapılmıştır. Toplam 50 örnek; 7 karpuz Citrullus lanatus, 3 kavun Cucumis melo L., 17 hıyar Cucumis sativus L., 8 kabak Cucurbita pepo, 9 kabak Cucurbita moschata, 2 su kabağı Lagenaria siceraria ve 4 acur Cucumis melo var. flexuous toplanmıştır. Örneklerde CMV varlığı, virüsün kısmi kılıf protein geninin 105 bç bölgesine spesifik problar ve primerler kullanılarak TaqMan real-time RT-PCR ile araştırılmıştır. Analizi yapılan 50 örneğin 6sında 12 CMV spesifik viral RNA tespit edilmiştir. CMV en sık Otluca alanında ve kabakgil çeşitleri arasında da kavunda 33.3 tespit edilmiştir. Merzandan toplanan örneklerde CMV etmenine rastlanmamıştır. Bu çalışmada kullanılan TaqMan real-time RT-PCR testi, CMVnin genel tespiti için bu yöntemin çok çeşitli konukçularda yaygın olarak kullanılabileceğini göstermiştir. Bu çalışma Türkiyenin Hakkari ilinde kabakgillerde CMVnin tespitine ilişkin ilk rapordur.

Article Details

Detection of Cucumber Mosaic Virus (CMV) in Cucurbits by TaqMan Real-Time RT-PCR in Hakkari Province, Türkiye

Authors:

Ayşenur Biltekin | Nevin Akdura

Publisher:

Mevlüt AKÇURA

Main Topics:

Phytopathology

Journal:

Turkish Journal of Agricultural and Natural Sciences Vol.11 Issue 3, pp.825-834

Document Type:

Article

Publication Date:

7/2024

Document Language:

English

Document ID (Org No.):

2001323712

pISSN:

2148-3647

Cucumber mosaic virus

cucurbits

TaqMan real-time RT-PCR

HakkariHıyar mozaik virüsü

kabakgiller

TaqMan real-time RT-PCR

Hakkari

Issues

Article Details

Authors: Ayşenur Biltekin | Nevin Akdura

Publisher: Mevlüt AKÇURA

Main Topics: Phytopathology

Journal: Turkish Journal of Agricultural and Natural Sciences Vol.11 Issue 3, pp.825-834

Document Type: Article

Publication Date: 7/2024

Document Language: English

Document ID (Org No.): 2001323712

pISSN: 2148-3647

Issues

Authors:

Ayşenur Biltekin | Nevin Akdura

Publisher:

Mevlüt AKÇURA

Main Topics:

Phytopathology

Journal:

Turkish Journal of Agricultural and Natural Sciences Vol.11 Issue 3, pp.825-834

Document Type:

Article

Publication Date:

7/2024

Document Language:

English

Document ID (Org No.):

2001323712

pISSN:

2148-3647