Can DNA Barcode Study be Done from a Museum Specimen Fixed in a Formaldehyde Solution? A Case of Emys orbicularis (Linnaeus, 1758)

Ulvi Kerem Günay; Batuhan Yaman Yakın; Atakan Pipilos; Emre Keskin; C. Varol Tok

Article Details

Can DNA Barcode Study be Done from a Museum Specimen Fixed in a Formaldehyde Solution? A Case of Emys orbicularis (Linnaeus, 1758)

Authors:

Ulvi Kerem Günay | Batuhan Yaman Yakın | Atakan Pipilos | Emre Keskin | C. Varol Tok

Publisher:

Çanakkale Onsekiz Mart University

Main Topics:

Ekoloji (Diğer)

Journal:

Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries Vol.7 Issue 1, pp.18-23

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001322764

pISSN:

2651-5326

Keywords:

Abstract:

 DNA barcoding, a molecular taxonomy technique, has been increasingly used by herptile taxonomists in recent years. In DNA barcoding studies with museum specimens, there are difficulties in achieving success in specimens that have been exposed to formaldehyde, which is usually used as a fixative, for a long time and intensively. Here we studied the effect of formaldehyde on the application of the DNA barcode method in Emys orbicularis specimens stored in 4 formaldehyde and 70 ethanol solution since 2008 and 2014. Sanger sequence analysis of tissues taken from samples stored in both ethanol and formaldehyde solution successfully yielded sequences of 623 bp. In conclusion, the use of ethanol solutions should be preferred for mid or long-term sample storage, especially in the context of molecular studies. In cases where the use of formaldehyde is unavoidable, it may be advisable to use extremely low concentrations to increase success in molecular research.

 DNA barcoding, a molecular taxonomy technique, has been increasingly used by herptile taxonomists in recent years. In DNA barcoding studies with museum specimens, there are difficulties in achieving success in specimens that have been exposed to formaldehyde, which is usually used as a fixative, for a long time and intensively. Here we studied the effect of formaldehyde on the application of the DNA barcode method in Emys orbicularis specimens stored in 4 formaldehyde and 70 ethanol solution since 2008 and 2014. Sanger sequence analysis of tissues taken from samples stored in both ethanol and formaldehyde solution successfully yielded sequences of 623 bp. In conclusion, the use of ethanol solutions should be preferred for mid or long-term sample storage, especially in the context of molecular studies. In cases where the use of formaldehyde is unavoidable, it may be advisable to use extremely low concentrations to increase success in molecular research.

 DNA barcoding, a molecular taxonomy technique, has been increasingly used by herptile taxonomists in recent years. In DNA barcoding studies with museum specimens, there are difficulties in achieving success in specimens that have been exposed to formaldehyde, which is usually used as a fixative, for a long time and intensively. Here we studied the effect of formaldehyde on the application of the DNA barcode method in Emys orbicularis specimens stored in 4 formaldehyde and 70 ethanol solution since 2008 and 2014. Sanger sequence analysis of tissues taken from samples stored in both ethanol and formaldehyde solution successfully yielded sequences of 623 bp. In conclusion, the use of ethanol solutions should be preferred for mid or long-term sample storage, especially in the context of molecular studies. In cases where the use of formaldehyde is unavoidable, it may be advisable to use extremely low concentrations to increase success in molecular research.

 Bir moleküler taksonomi tekniği olan DNA barkodlama, son yıllarda herptil taksonomistleri tarafından giderek daha fazla kullanılmaktadır. Müze örnekleri ile yapılan DNA barkodlama çalışmalarında, genellikle fiksatif olarak kullanılan formaldehite uzun süre ve yoğun bir şekilde maruz kalan örneklerde başarı elde etmekte zorluklar yaşanmaktadır. Bu çalışmada, herptil koleksiyon materyali olarak 2008 ve 2014 yıllarından beri 4 formaldehit ve 70 etanol çözeltisinde saklanan Emys orbicularis örneklerinde formaldehitin DNA barkod yönteminin uygulanması üzerindeki etkisi araştırılmıştır. Hem etanol hem de formaldehit çözeltisinde saklanan örneklerden alınan dokuların Sanger dizi analizi sonucunda 623 baz çiftinden oluşan dizileri başarıyla elde edilmiştir. Sonuç olarak, özellikle moleküler çalışmalar bağlamında orta ve uzun süreli numune saklama için alkol solüsyonlarının kullanılmasının tercih edilmesi gerekmektedir. Formaldehit kullanımının kaçınılmaz olduğu durumlarda ise, moleküler araştırmalarda başarıyı artırmak için son derece düşük konsantrasyonların kullanılması tavsiye edilebilir.

Article Details

Can DNA Barcode Study be Done from a Museum Specimen Fixed in a Formaldehyde Solution? A Case of Emys orbicularis (Linnaeus, 1758)

Authors:

Ulvi Kerem Günay | Batuhan Yaman Yakın | Atakan Pipilos | Emre Keskin | C. Varol Tok

Publisher:

Çanakkale Onsekiz Mart University

Main Topics:

Ekoloji (Diğer)

Journal:

Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries Vol.7 Issue 1, pp.18-23

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001322764

pISSN:

2651-5326

Cytochrome oxidase I

European pond turtle

DNA barcoding

Collection

TürkiyeSitokrom oksidaz I

Benekli kaplumbağa

DNA bakrodlama

Biyolojik koleksiyon

Türkiye

Issues

Article Details

Authors: Ulvi Kerem Günay | Batuhan Yaman Yakın | Atakan Pipilos | Emre Keskin | C. Varol Tok

Publisher: Çanakkale Onsekiz Mart University

Main Topics: Ekoloji (Diğer)

Journal: Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries Vol.7 Issue 1, pp.18-23

Document Type: Article

Publication Date: 10/2024

Document Language: English

Document ID (Org No.): 2001322764

pISSN: 2651-5326

Issues

Authors:

Ulvi Kerem Günay | Batuhan Yaman Yakın | Atakan Pipilos | Emre Keskin | C. Varol Tok

Publisher:

Çanakkale Onsekiz Mart University

Main Topics:

Ekoloji (Diğer)

Journal:

Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries Vol.7 Issue 1, pp.18-23

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001322764

pISSN:

2651-5326