Evaluation of in vitro antioxidative and protective effects of kefir on cyclophosphamide-upon oxidative stress and lung damage in rats

Songül Çetik Yıldız; Cemil Demir; Mustafa Cengiz; Betül Peker Cengiz; Adnan Ayhancı

Article Details

Evaluation of in vitro antioxidative and protective effects of kefir on cyclophosphamide-upon oxidative stress and lung damage in rats

Authors:

Songül Çetik Yıldız | Cemil Demir | Mustafa Cengiz | Betül Peker Cengiz | Adnan Ayhancı

Publisher:

Bingol University

Main Topics:

Kanser Biyolojisi

Journal:

Bingöl Üniversitesi Sağlık Dergisi Vol.5 Issue 1, pp.11-18

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001315252

pISSN:

2717-7653

Keywords:

Abstract:

 Purpose: Kefir, a natural probiotic, has antioxidative and antitumor properties, as well as potential beneficial properties resulting from microbial species and fermentation. Cyclophosphamide CYP is a cytotoxic drug that is highly preferred in cancer therapy but can suppress both humoral and cellular immunity. The use of effective doses is limited because CYP has an immunosuppressive effect and causes multiple organ toxicity such as the lungs, and the use of effective high doses is restricted. Methods: Wistar albino rats were divided into 6 groups. As; control, 150 mgkg CYP, 5 mgkg kefir, 5 mgkg kefir 150 CYP, l0 mgkg kefir, l0 mgkg kefir150 CYP. Fermented kefirs from the 1st, 2nd and 3rd days were mixed and given to the rats for 12 days, while CYP was given as a single dose and ip on the 12th day of the experiment. The received parameters were evaluated. Results: It was observed that the OSI value, which indicates oxidative stress, increased in the CYP-administered group, and this level approached control in the CYPkefir groups. In addition, while degeneration, intense inflammation in the interalveolar area, collapse of the alveoli, and intense inflammation around the bronchioles were observed in the lung parenchyma of CYP-administered rats, it was observed that these effects were effectively improved in the groups given kefirCYP. Conclusion: In conclusion, in our study, it was observed that kefir showed antioxidative and protective effects on CYP-induced oxidative stress, congestion, alveolar damage and inflammation.

 Purpose: Kefir, a natural probiotic, has antioxidative and antitumor properties, as well as potential beneficial properties resulting from microbial species and fermentation. Cyclophosphamide CYP is a cytotoxic drug that is highly preferred in cancer therapy but can suppress both humoral and cellular immunity. The use of effective doses is limited because CYP has an immunosuppressive effect and causes multiple organ toxicity such as the lungs, and the use of effective high doses is restricted. Methods: Wistar albino rats were divided into 6 groups. As; control, 150 mgkg CYP, 5 mgkg kefir, 5 mgkg kefir 150 CYP, l0 mgkg kefir, l0 mgkg kefir150 CYP. Fermented kefirs from the 1st, 2nd and 3rd days were mixed and given to the rats for 12 days, while CYP was given as a single dose and ip on the 12th day of the experiment. The received parameters were evaluated. Results: It was observed that the OSI value, which indicates oxidative stress, increased in the CYP-administered group, and this level approached control in the CYPkefir groups. In addition, while degeneration, intense inflammation in the interalveolar area, collapse of the alveoli, and intense inflammation around the bronchioles were observed in the lung parenchyma of CYP-administered rats, it was observed that these effects were effectively improved in the groups given kefirCYP. Conclusion: In conclusion, in our study, it was observed that kefir showed antioxidative and protective effects on CYP-induced oxidative stress, congestion, alveolar damage and inflammation.

 Purpose: Kefir, a natural probiotic, has antioxidative and antitumor properties, as well as potential beneficial properties resulting from microbial species and fermentation. Cyclophosphamide CYP is a cytotoxic drug that is highly preferred in cancer therapy but can suppress both humoral and cellular immunity. The use of effective doses is limited because CYP has an immunosuppressive effect and causes multiple organ toxicity such as the lungs, and the use of effective high doses is restricted. Methods: Wistar albino rats were divided into 6 groups. As; control, 150 mgkg CYP, 5 mgkg kefir, 5 mgkg kefir 150 CYP, l0 mgkg kefir, l0 mgkg kefir150 CYP. Fermented kefirs from the 1st, 2nd and 3rd days were mixed and given to the rats for 12 days, while CYP was given as a single dose and ip on the 12th day of the experiment. The received parameters were evaluated. Results: It was observed that the OSI value, which indicates oxidative stress, increased in the CYP-administered group, and this level approached control in the CYPkefir groups. In addition, while degeneration, intense inflammation in the interalveolar area, collapse of the alveoli, and intense inflammation around the bronchioles were observed in the lung parenchyma of CYP-administered rats, it was observed that these effects were effectively improved in the groups given kefirCYP. Conclusion: In conclusion, in our study, it was observed that kefir showed antioxidative and protective effects on CYP-induced oxidative stress, congestion, alveolar damage and inflammation.

 Amaç: Doğal bir probiyotik olan kefir, antioksidatif ve antitümör özelliklerinin yanı sıra mikrobiyal türler ve fermantasyondan kaynaklanan yararlı özelliklere de sahiptir. Siklofosfamid SF, kanser tedavisinde oldukça tercih edilen ancak hem humoral hem de hücresel bağışıklığı baskılayabilen sitotoksik bir ilaçtır. SFnin immünsüpresif etkisinin olması ve akciğer gibi çoklu organ toksisitesine neden olması nedeniyle etkili yüksek dozda kullanımı sınırlıdır. Yöntem: Wistar albino sıçanlar 6 gruba ayrıldı. Kontrol, 150 mgkg SF, 5 mgkg kefir, 5 mgkg kefir 150 SF, 10 mgkg kefir, 10 mgkg kefir150 SF. Ratlara 1., 2. ve 3. günlerdeki fermente kefirler karıştırılarak 12 gün süreyle verilirken, deneyin 12. gününde SF tek doz ve i.p. olarak verildi. Alınan kan ve doku parametreleri değerlendirildi. Bulgular: Oksidatif stresi gösteren OSI değerinin SF uygulanan grupta arttığı, SFkefir gruplarında ise bu düzeyin kontrole yaklaştığı görüldü. Ayrıca SF uygulanan sıçanların akciğer parankiminde dejenerasyon, interalveoler bölgede yoğun inflamasyon, alveollerde kollaps ve bronşiyol çevresinde yoğun inflamasyon gözlenirken, kefir verilen gruplarda bu etkilerin etkin bir şekilde düzeldiği gözlendi. Sonuç: Sonuç olarak deneysel çalışmamızda kefirin SF kaynaklı oksidatif stres, konjesyon, alveolar hasar ve inflamasyon üzerinde antioksidatif ve koruyucu etkiler gösterdiği gözlendi.

Article Details

Evaluation of in vitro antioxidative and protective effects of kefir on cyclophosphamide-upon oxidative stress and lung damage in rats

Authors:

Songül Çetik Yıldız | Cemil Demir | Mustafa Cengiz | Betül Peker Cengiz | Adnan Ayhancı

Publisher:

Bingol University

Main Topics:

Kanser Biyolojisi

Journal:

Bingöl Üniversitesi Sağlık Dergisi Vol.5 Issue 1, pp.11-18

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001315252

pISSN:

2717-7653

Kefir

cyclophosphamide

antioxidant

oxidative stress

lung damage

ratsKefir

siklofosfamid

antioksidan

oksidatif stres

akciğer hasarı

sıçan

Issues

Article Details

Authors: Songül Çetik Yıldız | Cemil Demir | Mustafa Cengiz | Betül Peker Cengiz | Adnan Ayhancı

Publisher: Bingol University

Main Topics: Kanser Biyolojisi

Journal: Bingöl Üniversitesi Sağlık Dergisi Vol.5 Issue 1, pp.11-18

Document Type: Article

Publication Date: 4/2024

Document Language: English

Document ID (Org No.): 2001315252

pISSN: 2717-7653

Issues

Authors:

Songül Çetik Yıldız | Cemil Demir | Mustafa Cengiz | Betül Peker Cengiz | Adnan Ayhancı

Publisher:

Bingol University

Main Topics:

Kanser Biyolojisi

Journal:

Bingöl Üniversitesi Sağlık Dergisi Vol.5 Issue 1, pp.11-18

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001315252

pISSN:

2717-7653