Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta

Cengiz Sarıkürkçü; Mustafa Cengiz; Gökhan Zengin

Article Details

Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta

Authors:

Cengiz Sarıkürkçü | Mustafa Cengiz | Gökhan Zengin

Publisher:

Marmara University

Main Topics:

Pharmaceutical Analytical Chemistry | Pharmacognosy

Journal:

Journal of Research in Pharmacy Vol.22 Issue 1, pp.86-90

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346058

pISSN:

2630-6344

Keywords:

Abstract:

 The members of the genus Phlomis have been traditionally used for therapeutic purposes in Turkey. In this study, the antioxidant properties of different extracts from P. leucophracta were investigated. Antioxidant properties were evaluated by different assays including free radical scavenging DPPH assay, reducing power potassium ferricyanide method, β-carotenelinoleic acid, metal chelating and phosphomolybdenum. Moreover, total phenolic and flavonoid contents were detected for each extracts. Total phenolic and flavonoid contents were detected as 30.86-55.00 mg GAEg extract and 4.93-26.09 mg QEg extract, respectively. The methanol and water extracts exhibited higher DPPH 1,1-diphenyl-2-picrylhydrazyl radical scavenging and reducing power abilities as compared to ethyl acetate and hexane extracts. The best activity was observed by the hexane extract in β-carotenelinoleic acid assay 94.35 at 2 mgmL. In metal chelating ability, those samples exhibited the following order at 0.25 mgmL concentration: Water 73.90Hexane64.87Ethyl acetate4.88Methanol 2.28. Based on our results, P. leucophracta may be utilized as a natural source of antioxidant compounds in food and pharmaceutical areas.

 The members of the genus Phlomis have been traditionally used for therapeutic purposes in Turkey. In this study, the antioxidant properties of different extracts from P. leucophracta were investigated. Antioxidant properties were evaluated by different assays including free radical scavenging DPPH assay, reducing power potassium ferricyanide method, β-carotenelinoleic acid, metal chelating and phosphomolybdenum. Moreover, total phenolic and flavonoid contents were detected for each extracts. Total phenolic and flavonoid contents were detected as 30.86-55.00 mg GAEg extract and 4.93-26.09 mg QEg extract, respectively. The methanol and water extracts exhibited higher DPPH 1,1-diphenyl-2-picrylhydrazyl radical scavenging and reducing power abilities as compared to ethyl acetate and hexane extracts. The best activity was observed by the hexane extract in β-carotenelinoleic acid assay 94.35 at 2 mgmL. In metal chelating ability, those samples exhibited the following order at 0.25 mgmL concentration: Water 73.90Hexane64.87Ethyl acetate4.88Methanol 2.28. Based on our results, P. leucophracta may be utilized as a natural source of antioxidant compounds in food and pharmaceutical areas.

 The members of the genus Phlomis have been traditionally used for therapeutic purposes in Turkey. In this study, the antioxidant properties of different extracts from P. leucophracta were investigated. Antioxidant properties were evaluated by different assays including free radical scavenging DPPH assay, reducing power potassium ferricyanide method, β-carotenelinoleic acid, metal chelating and phosphomolybdenum. Moreover, total phenolic and flavonoid contents were detected for each extracts. Total phenolic and flavonoid contents were detected as 30.86-55.00 mg GAEg extract and 4.93-26.09 mg QEg extract, respectively. The methanol and water extracts exhibited higher DPPH 1,1-diphenyl-2-picrylhydrazyl radical scavenging and reducing power abilities as compared to ethyl acetate and hexane extracts. The best activity was observed by the hexane extract in β-carotenelinoleic acid assay 94.35 at 2 mgmL. In metal chelating ability, those samples exhibited the following order at 0.25 mgmL concentration: Water 73.90Hexane64.87Ethyl acetate4.88Methanol 2.28. Based on our results, P. leucophracta may be utilized as a natural source of antioxidant compounds in food and pharmaceutical areas.

 The members of the genus Phlomis have been traditionally used for therapeutic purposes in Turkey. In this study, the antioxidant properties of different extracts from P. leucophracta were investigated. Antioxidant properties were evaluated by different assays including free radical scavenging DPPH assay, reducing power potassium ferricyanide method, β-carotenelinoleic acid, metal chelating and phosphomolybdenum. Moreover, total phenolic and flavonoid contents were detected for each extracts. Total phenolic and flavonoid contents were detected as 30.86-55.00 mg GAEg extract and 4.93-26.09 mg QEg extract, respectively. The methanol and water extracts exhibited higher DPPH 1,1-diphenyl-2-picrylhydrazyl radical scavenging and reducing power abilities as compared to ethyl acetate and hexane extracts. The best activity was observed by the hexane extract in β-carotenelinoleic acid assay 94.35 at 2 mgmL. In metal chelating ability, those samples exhibited the following order at 0.25 mgmL concentration: Water 73.90Hexane64.87Ethyl acetate4.88Methanol 2.28. Based on our results, P. leucophracta may be utilized as a natural source of antioxidant compounds in food and pharmaceutical areas.

Article Details

Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta

Authors:

Cengiz Sarıkürkçü | Mustafa Cengiz | Gökhan Zengin

Publisher:

Marmara University

Main Topics:

Pharmaceutical Analytical Chemistry | Pharmacognosy

Journal:

Journal of Research in Pharmacy Vol.22 Issue 1, pp.86-90

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346058

pISSN:

2630-6344

Phlomis leucophracta

phenolics

DPPH

antioxidant activity

reducing power

Issues

Article Details

Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta

Authors: Cengiz Sarıkürkçü | Mustafa Cengiz | Gökhan Zengin

Publisher: Marmara University

Main Topics: Pharmaceutical Analytical Chemistry | Pharmacognosy

Journal: Journal of Research in Pharmacy Vol.22 Issue 1, pp.86-90

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001346058

pISSN: 2630-6344

Phlomis leucophracta

phenolics

DPPH

antioxidant activity

reducing power

Issues

Authors:

Cengiz Sarıkürkçü | Mustafa Cengiz | Gökhan Zengin

Publisher:

Marmara University

Main Topics:

Pharmaceutical Analytical Chemistry | Pharmacognosy

Journal:

Journal of Research in Pharmacy Vol.22 Issue 1, pp.86-90

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346058

pISSN:

2630-6344