Salivary Protease Activity in Children with Cystic Fibrosis

Timur Köse

Article Details

Salivary Protease Activity in Children with Cystic Fibrosis

Authors:

Timur Köse

Publisher:

Aydin Adnan Menderes University

Main Topics:

Dentistry (Other)

Journal:

Meandros Medical And Dental Journal Vol.24 Issue 1, pp.19-25

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001348371

pISSN:

2149-9063

Keywords:

Abstract:

 Objective: Patients with cystic fibrosis CF present with impaired protease-antiprotease balance in their lungs. However, salivary protease equilibrium in children with CF with poor oral health has not been reported. The current study investigated salivary matrix metalloproteinase-8 MMP-8, tissue inhibitor of matrix metalloproteinases-1 TIMP-1, neutrophil elastase NE, and myeloperoxidase MPO levels in children with CF with or without gingivitis. Materials and Methods: Eleven CF and 11 systemically healthy children aged 3-12 years were evaluated. Clinical periodontal examinations including probing pocket depth PPD, gingival index GI, plaque index PI, and bleeding on probing BOP were recorded and saliva samples were obtained. Salivary MMP-8, TIMP-1, NE, and MPO levels were analyzed by immunofluorometric assay and enzyme-linked immunosorbent assay. Results: Salivary levels of MMP-8, TIMP-1, NE, MPO, and MMP-8TIMP-1 molar ratios were similar in CF and systemically healthy children p0.05. Levels of MMP-8, NE, and MPO were significantly higher in the saliva of children with gingivitis compared to periodontally healthy children in both CF and systemically healthy groups p0.05. MMP-8 and MMP-8TIMP-1 levels were positively correlated with GI, PI, and BOP p0.05, while NE and MPO levels were related to all periodontal parameters p0.01. Conclusion: CF may not alter the activity of MMP-8, NE, and MPO. On the other hand, gingival inflammation had a pronounced effect on salivary levels of these enzymes and the MMP-8TIMP-1 molar ratio in children, irrespective of CF. Further investigations in larger cohorts are needed to better clarify this issue.

 Objective: Patients with cystic fibrosis CF present with impaired protease-antiprotease balance in their lungs. However, salivary protease equilibrium in children with CF with poor oral health has not been reported. The current study investigated salivary matrix metalloproteinase-8 MMP-8, tissue inhibitor of matrix metalloproteinases-1 TIMP-1, neutrophil elastase NE, and myeloperoxidase MPO levels in children with CF with or without gingivitis. Materials and Methods: Eleven CF and 11 systemically healthy children aged 3-12 years were evaluated. Clinical periodontal examinations including probing pocket depth PPD, gingival index GI, plaque index PI, and bleeding on probing BOP were recorded and saliva samples were obtained. Salivary MMP-8, TIMP-1, NE, and MPO levels were analyzed by immunofluorometric assay and enzyme-linked immunosorbent assay. Results: Salivary levels of MMP-8, TIMP-1, NE, MPO, and MMP-8TIMP-1 molar ratios were similar in CF and systemically healthy children p0.05. Levels of MMP-8, NE, and MPO were significantly higher in the saliva of children with gingivitis compared to periodontally healthy children in both CF and systemically healthy groups p0.05. MMP-8 and MMP-8TIMP-1 levels were positively correlated with GI, PI, and BOP p0.05, while NE and MPO levels were related to all periodontal parameters p0.01. Conclusion: CF may not alter the activity of MMP-8, NE, and MPO. On the other hand, gingival inflammation had a pronounced effect on salivary levels of these enzymes and the MMP-8TIMP-1 molar ratio in children, irrespective of CF. Further investigations in larger cohorts are needed to better clarify this issue.

 Objective: Patients with cystic fibrosis CF present with impaired protease-antiprotease balance in their lungs. However, salivary protease equilibrium in children with CF with poor oral health has not been reported. The current study investigated salivary matrix metalloproteinase-8 MMP-8, tissue inhibitor of matrix metalloproteinases-1 TIMP-1, neutrophil elastase NE, and myeloperoxidase MPO levels in children with CF with or without gingivitis. Materials and Methods: Eleven CF and 11 systemically healthy children aged 3-12 years were evaluated. Clinical periodontal examinations including probing pocket depth PPD, gingival index GI, plaque index PI, and bleeding on probing BOP were recorded and saliva samples were obtained. Salivary MMP-8, TIMP-1, NE, and MPO levels were analyzed by immunofluorometric assay and enzyme-linked immunosorbent assay. Results: Salivary levels of MMP-8, TIMP-1, NE, MPO, and MMP-8TIMP-1 molar ratios were similar in CF and systemically healthy children p0.05. Levels of MMP-8, NE, and MPO were significantly higher in the saliva of children with gingivitis compared to periodontally healthy children in both CF and systemically healthy groups p0.05. MMP-8 and MMP-8TIMP-1 levels were positively correlated with GI, PI, and BOP p0.05, while NE and MPO levels were related to all periodontal parameters p0.01. Conclusion: CF may not alter the activity of MMP-8, NE, and MPO. On the other hand, gingival inflammation had a pronounced effect on salivary levels of these enzymes and the MMP-8TIMP-1 molar ratio in children, irrespective of CF. Further investigations in larger cohorts are needed to better clarify this issue.

 Objective: Patients with cystic fibrosis CF present with impaired protease-antiprotease balance in their lungs. However, salivary protease equilibrium in children with CF with poor oral health has not been reported. The current study investigated salivary matrix metalloproteinase-8 MMP-8, tissue inhibitor of matrix metalloproteinases-1 TIMP-1, neutrophil elastase NE, and myeloperoxidase MPO levels in children with CF with or without gingivitis. Materials and Methods: Eleven CF and 11 systemically healthy children aged 3-12 years were evaluated. Clinical periodontal examinations including probing pocket depth PPD, gingival index GI, plaque index PI, and bleeding on probing BOP were recorded and saliva samples were obtained. Salivary MMP-8, TIMP-1, NE, and MPO levels were analyzed by immunofluorometric assay and enzyme-linked immunosorbent assay. Results: Salivary levels of MMP-8, TIMP-1, NE, MPO, and MMP-8TIMP-1 molar ratios were similar in CF and systemically healthy children p0.05. Levels of MMP-8, NE, and MPO were significantly higher in the saliva of children with gingivitis compared to periodontally healthy children in both CF and systemically healthy groups p0.05. MMP-8 and MMP-8TIMP-1 levels were positively correlated with GI, PI, and BOP p0.05, while NE and MPO levels were related to all periodontal parameters p0.01. Conclusion: CF may not alter the activity of MMP-8, NE, and MPO. On the other hand, gingival inflammation had a pronounced effect on salivary levels of these enzymes and the MMP-8TIMP-1 molar ratio in children, irrespective of CF. Further investigations in larger cohorts are needed to better clarify this issue.

Article Details

Salivary Protease Activity in Children with Cystic Fibrosis

Authors:

Timur Köse

Publisher:

Aydin Adnan Menderes University

Main Topics:

Dentistry (Other)

Journal:

Meandros Medical And Dental Journal Vol.24 Issue 1, pp.19-25

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001348371

pISSN:

2149-9063

Gingivitis

matrix metalloproteinases

leukocyte elastase

cystic fibrosis

biomarkers

saliva

Issues

Article Details

Salivary Protease Activity in Children with Cystic Fibrosis

Authors: Timur Köse

Publisher: Aydin Adnan Menderes University

Main Topics: Dentistry (Other)

Journal: Meandros Medical And Dental Journal Vol.24 Issue 1, pp.19-25

Document Type: Article

Publication Date: 3/2023

Document Language: English

Document ID (Org No.): 2001348371

pISSN: 2149-9063

Gingivitis

matrix metalloproteinases

leukocyte elastase

cystic fibrosis

biomarkers

saliva

Issues

Authors:

Timur Köse

Publisher:

Aydin Adnan Menderes University

Main Topics:

Dentistry (Other)

Journal:

Meandros Medical And Dental Journal Vol.24 Issue 1, pp.19-25

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001348371

pISSN:

2149-9063