Evaluation of Various Sunflower ( Helianthus annuus L.) Genotypes for Agrobacterium tumefaciens-mediated Gene Transfer

Ekrem GÜREL

Article Details

Evaluation of Various Sunflower ( Helianthus annuus L.) Genotypes for Agrobacterium tumefaciens-mediated Gene Transfer

Authors:

Ekrem GÜREL

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 1999 Vol.23 Issue 3, pp.171-178

Document Type:

Article

Publication Date:

2/1999

Document Language:

Turkish

Document ID (Org No.):

2000724762

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X

Abstract:

 

In this work, 10 sunflower ( Helianthus annuus L.) genotypes were evaluated for their suitability for Agrobacterium  tumefaciens-mediated gene transfer based on detection of a reported gene expression (GUS). A number of factors including the type  of shoot-tip explant (split vs. intact), bacterial strain/binary vector combinations and wounding intact shoot-tips by particle  bombardment were examined. Genotypes showed significant differences in their transformation efficiencies ranging from 0.0 to  82.7% GUS positive explants, hybrid genotypes being more responsive to Agrobacterium infection than inbred lines. Use of split  shoot-tip explants did not increase the transformation efficiency over intact explants but the AGL-1/pKIWI strain/vector combination was more effective than the LBA4404/pTOK233 combination. Wounding explants by particle bombardment prior to inoculations with Agrobacterium had no positive effect on transformation.

 

In this work, 10 sunflower ( Helianthus annuus L.) genotypes were evaluated for their suitability for Agrobacterium  tumefaciens-mediated gene transfer based on detection of a reported gene expression (GUS). A number of factors including the type  of shoot-tip explant (split vs. intact), bacterial strain/binary vector combinations and wounding intact shoot-tips by particle  bombardment were examined. Genotypes showed significant differences in their transformation efficiencies ranging from 0.0 to  82.7% GUS positive explants, hybrid genotypes being more responsive to Agrobacterium infection than inbred lines. Use of split  shoot-tip explants did not increase the transformation efficiency over intact explants but the AGL-1/pKIWI strain/vector combination was more effective than the LBA4404/pTOK233 combination. Wounding explants by particle bombardment prior to inoculations with Agrobacterium had no positive effect on transformation.

Article Details

Evaluation of Various Sunflower ( Helianthus annuus L.) Genotypes for Agrobacterium tumefaciens-mediated Gene Transfer

Authors: Ekrem GÜREL

Publisher: TUBITAK

Journal: Turkish Journal of Botany 1999 Vol.23 Issue 3, pp.171-178

Document Type: Article

Publication Date: 2/1999

Document Language: Turkish

Document ID (Org No.): 2000724762

Frequency: Bimonthly

eISSN: 1303-6106

pISSN: 1300-008X

Issues

Authors:

Ekrem GÜREL

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 1999 Vol.23 Issue 3, pp.171-178

Document Type:

Article

Publication Date:

2/1999

Document Language:

Turkish

Document ID (Org No.):

2000724762

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X