A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Rasheed; et al.

doi:10.36103/ijas.v51i6.1183

Article Details

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Authors:

Rasheed | et al.

Publisher:

University of Baghdad

Journal:

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.51 Issue 6, pp.0-0

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001372629

pISSN:

2410-0862

DOI:

10.36103/ijas.v51i6.1183

Keywords:

Abstract:

 The present study was designed to investigate the possibility of using Lactobacillus as a skin probiotic. For this purpose, 63 isolates of Lactobacillus sp. were collected from dairy products and human mouth samples. The isolates were screened for bacteriocin production by primary and secondary screening against three common skin pathogens. The results showed that the isolate Lactobacillus HT1give the highest productivity of bacteriocin (320AU/ml), the efficient isolate was identified through molecular detection using 16S ribosomal RNA gene. In addition, result showed that L. acidophilus HT1 was un encapsulated; has no capability to produce biofilm and was susceptible to amoxicillin–clavulanic acid, vancomycin and chloramphenicol. Moreover, the infectious dose of L. acidophilus HT1 was studied and results revealed that mice were remained in a healthy condition including all vital processes such as breathing, consuming food and water as well as external and anatomical appearance. Next, an emul gel formula contained L. acidophilus HT1 biomass was prepared which already examined in vitro and in vivo. Our results confirmed the efficacy of the formula via inhibiting the growth of pathogenic isolates and through several points included: viability of L. acidophilus HT1 cells, its ability to produce bacteriocin, and its releasing from the formula to the external environment without trapping or interaction among bacteriocin and any component of formula. In vivo results approved the affectivity of L. acidophilus HT1 biomass to treat the wounds infected with different bacterial pathogens during seven days after the onset of treatment compared with the control groups.

Article Details

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Authors:

Rasheed | et al.

Publisher:

University of Baghdad

Journal:

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.51 Issue 6, pp.0-0

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001372629

pISSN:

2410-0862

DOI:

10.36103/ijas.v51i6.1183

bacteriocin

emul gel formula

skin disease

bacteriocin application

Issues

Article Details

A PROBIOTIC APPLICATION OF Lactobacillus acidophilus HT1 FOR THE TREATMENT OF SOME SKIN PATHOGENS

Authors: Rasheed | et al.

Publisher: University of Baghdad

Journal: IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.51 Issue 6, pp.0-0

Document Type: Article

Publication Date: 12/2020

Document Language: English

Document ID (Org No.): 2001372629

pISSN: 2410-0862

DOI: 10.36103/ijas.v51i6.1183

bacteriocin

emul gel formula

skin disease

bacteriocin application

Issues

Authors:

Rasheed | et al.

Publisher:

University of Baghdad

Journal:

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Vol.51 Issue 6, pp.0-0

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001372629

pISSN:

2410-0862

DOI:

10.36103/ijas.v51i6.1183