DEVELOPMENT OF A UREA BIOSENSOR BASED ON A POLYMERIC MEMBRANE INCLUDING ZEOLITE

Hamlaoui M. L; Bouyahia N; Jaffrezic-renault N

Article Details

DEVELOPMENT OF A UREA BIOSENSOR BASED ON A POLYMERIC MEMBRANE INCLUDING ZEOLITE

Authors:

Hamlaoui M. L | Bouyahia N | Jaffrezic-renault N

Publisher:

Université Mentouri de Constantine

Journal:

Sciences & technologie. A, sciences exactes Issue 27, pp.51-55

Document Type:

Article

Publication Date:

6/2008

Document Language:

English

Document ID (Org No.):

2001909443

pISSN:

2602-7739

Keywords:

Abstract:

 A urea biosensor has been prepared by covalent biding of urease directly to the surface of
an ammonium-sensitive field effect transistor (FET).The NH4
+ -sensitive membrane is
based on a zeolite-incorporated polymeric membrane biosensor (clinoptilolite). The
sensitivity of ammonium detection is sub-nernstian (32mV/pNH4
+) but the ISFET presents
a high selectivity, which is interesting for measurements in biological media. The grafting
of urease to the NH4
+-sensitive membrane was permorfed by cross-linking with
glutaraldehyde .The sensitivity of the urea ENFET is 15V/purea and this remains stable
over 15 days with a detection limit of 3x10-5 M. Finally, in order to test feasibility of the
urea biosensor for environmental applications, the remaining activity of the urease was
determined after exposure to enzyme inhibiting heavy metals ions such as Hg(II).Using
these urea biosensors, a detection limit of less than 5 x 10-8 M was obtained for Hg(II).

Article Details

DEVELOPMENT OF A UREA BIOSENSOR BASED ON A POLYMERIC MEMBRANE INCLUDING ZEOLITE

Authors:

Hamlaoui M. L | Bouyahia N | Jaffrezic-renault N

Publisher:

Université Mentouri de Constantine

Journal:

Sciences & technologie. A, sciences exactes Issue 27, pp.51-55

Document Type:

Article

Publication Date:

6/2008

Document Language:

English

Document ID (Org No.):

2001909443

pISSN:

2602-7739

ISFET

Urea biosensor

Zeolite

Polymeric membrane

Issues

Article Details

DEVELOPMENT OF A UREA BIOSENSOR BASED ON A POLYMERIC MEMBRANE INCLUDING ZEOLITE

Authors: Hamlaoui M. L | Bouyahia N | Jaffrezic-renault N

Publisher: Université Mentouri de Constantine

Journal: Sciences & technologie. A, sciences exactes Issue 27, pp.51-55

Document Type: Article

Publication Date: 6/2008

Document Language: English

Document ID (Org No.): 2001909443

pISSN: 2602-7739

ISFET

Urea biosensor

Zeolite

Polymeric membrane

Issues

Authors:

Hamlaoui M. L | Bouyahia N | Jaffrezic-renault N

Publisher:

Université Mentouri de Constantine

Journal:

Sciences & technologie. A, sciences exactes Issue 27, pp.51-55

Document Type:

Article

Publication Date:

6/2008

Document Language:

English

Document ID (Org No.):

2001909443

pISSN:

2602-7739