Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum

Chunyan FENG; Ya HUANG; Yunsong YU; Guorong DUAN; Yuying DAI; Ke DONG; Qingtian LI

Article Details

Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum

Authors:

Chunyan FENG | Ya HUANG | Yunsong YU | Guorong DUAN | Yuying DAI | Ke DONG | Qingtian LI

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 1, pp.55-59

Document Type:

Article

Publication Date:

3/2015

Document Language:

Turkish

Document ID (Org No.):

2000752853

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

To detect the effects on quinolone resistance due to biofilm formation activity in Ureaplasma urealyticum.  Materials and methods: An improved checkerboard dilution susceptibility test was used to analyze the quinolone resistance of clinical Ureaplasma isolates and a postbiofilm formation susceptibility assay was used to compare the effects on the quinolone susceptibility between pre- and postbiofilm formations. Several important functional gene expressions were detected to evaluate their roles in the process of the quinolone resistance mechanism.  Results: The quinolone-resistant isolates produced more biofilms than the sensitive isolates. In both the quinolone-resistant and -sensitive groups, the minimal inhibitory concentrations after biofilm formation were higher than those before biofilm formation. The expression of the metabolism-related gene ureC in postbiofilm formation was higher than those in prebiofilm formation.  Conclusion:  It seems that biofilm formation is quite important in the generation of quinolone resistance in Ureaplasma. It is very useful to detect biofilm formation activity as well as to analyze other laboratory parameters for Ureaplasma.

 

To detect the effects on quinolone resistance due to biofilm formation activity in Ureaplasma urealyticum.  Materials and methods: An improved checkerboard dilution susceptibility test was used to analyze the quinolone resistance of clinical Ureaplasma isolates and a postbiofilm formation susceptibility assay was used to compare the effects on the quinolone susceptibility between pre- and postbiofilm formations. Several important functional gene expressions were detected to evaluate their roles in the process of the quinolone resistance mechanism.  Results: The quinolone-resistant isolates produced more biofilms than the sensitive isolates. In both the quinolone-resistant and -sensitive groups, the minimal inhibitory concentrations after biofilm formation were higher than those before biofilm formation. The expression of the metabolism-related gene ureC in postbiofilm formation was higher than those in prebiofilm formation.  Conclusion:  It seems that biofilm formation is quite important in the generation of quinolone resistance in Ureaplasma. It is very useful to detect biofilm formation activity as well as to analyze other laboratory parameters for Ureaplasma.

Article Details

Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum

Authors:

Chunyan FENG | Ya HUANG | Yunsong YU | Guorong DUAN | Yuying DAI | Ke DONG | Qingtian LI

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 1, pp.55-59

Document Type:

Article

Publication Date:

3/2015

Document Language:

Turkish

Document ID (Org No.):

2000752853

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Quinolone resistance

biofilm formation activity

Ureaplasma urealyticum

Issues

Article Details

Effects on quinolone resistance due to the biofilm formation activity in Ureaplasma urealyticum

Authors: Chunyan FENG | Ya HUANG | Yunsong YU | Guorong DUAN | Yuying DAI | Ke DONG | Qingtian LI

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 1, pp.55-59

Document Type: Article

Publication Date: 3/2015

Document Language: Turkish

Document ID (Org No.): 2000752853

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Quinolone resistance

biofilm formation activity

Ureaplasma urealyticum

Issues

Authors:

Chunyan FENG | Ya HUANG | Yunsong YU | Guorong DUAN | Yuying DAI | Ke DONG | Qingtian LI

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 1, pp.55-59

Document Type:

Article

Publication Date:

3/2015

Document Language:

Turkish

Document ID (Org No.):

2000752853

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144