IN VITRO ACTIVITY OF QUINUPRISTIN-DALFOPRISTIN, METHICILLIN AND VANCOMYCIN AGAINST STAPHYLOCOCCUS STRAINS ISOLATED FROM CLINICAL SAMPLES

Erkan YULA; Turkan TOKA ÖZER; Ozcan DEVECİ; Alicem TEKİN; Keramettin YANIK; Suleyman DURMAZ

Article Details

IN VITRO ACTIVITY OF QUINUPRISTIN-DALFOPRISTIN, METHICILLIN AND VANCOMYCIN AGAINST STAPHYLOCOCCUS STRAINS ISOLATED FROM CLINICAL SAMPLES

Authors:

Erkan YULA | Turkan TOKA ÖZER | Ozcan DEVECİ | Alicem TEKİN | Keramettin YANIK | Suleyman DURMAZ

Publisher:

Erciyes University

Journal:

Journal of Health Sciences 2012 Vol.21 Issue 1, pp.45-49

Document Type:

Article

Publication Date:

2/2012

Document Language:

Turkish

Document ID (Org No.):

2000614358

Frequency:

Tri-annual

pISSN:

1018-3655

Abstract:

 

The aim of the study is to investigate susceptibility of staphylococci strains isolated from various clinical samples to quinupristin-dalfopristin. The ninety-eight strains staphylococci (CNSs) and 24 S. aureus] isolated from various clinical samples were included the study which had been sent to microbiology laboratory. Staphylococci strains were identified by using conventional methods. Methicillin and quinupristin-dalfopristin susceptibility of staphylococci strains were performed by Kirby-Bauer’s disc diffusion method according to the Clinical and Laboratory Standards Institute criteria. Also, vancomycin susceptibility of strains was investigated by E-test method. Strain of S. aureus ATCC 25923 was used as the quality control strain. The fifty-three (72%) strains of the CNSs were defined as methicillinresistant CNS (MR-CNS), three (13%) strains of S. aureus was defined as methicillin-resistant S. aureus (MRSA). The eight (15%) strains of MR-CNS were found resistant to quinupristin-dalfopristin, one (5%) strain of MS-CNS were found resistant to quinupristin-dalfopristin. None of MSSA or MRSA strains were resistant to quinupristin-dalfopristin. All of the strains were found as susceptible to vancomycin. Strains of staphylococci were found susceptible to quinupristin-dalfopristin at high rates. Consequently we think that quinupristin-dalfopristin combination may be an alternative option for treatment of resistant Gram-positive cocci infections like vancomycin.

 

Bu çalışmada, çeşitli klinik örneklerden izole edilen stafilokok suşlarında kinupristin-dalfopristin duyarlılık oranlarının araştırılması amaçlanmıştır. Çalışmaya, mikrobiyoloji laboratuvarına gönderilen çeşitli klinik örneklerden izole edilen 98 stafilokok suşu [74'ü koagülaz negatif stafilokok (KNS) ve 24'ü S. aureus] dahil edildi. Stafilokok suşları konvansiyonel yöntemler ile tanımlandı. Stafilokok suşlarının metisilin ve kinupristin-dalfopristin duyarlılığı Clinical and Laboratory Standards Institute (CLSI) önerileri doğrultusunda Kirby-Bauer disk difüzyon yöntemiyle çalışıldı. Ayrıca suşların vankomisin duyarlılığı E-test yöntemi ile araştırıldı. Çalışmada kalite kontrol suşu olarak S. aureus ATCC 25923 kullanıldı. İzole edilen KNS’lerin 53(% 72)’ü metisiline dirençli KNS (MR-KNS) ve S. aureus’ların ise 3(%13)’ü metisiline dirençli (MRSA) olarak tanımlandı. MRKNS’lerin 8(% 15)’i kinupristin-dalfopristine dirençli iken, metisiline duyarlı KNS'lerde (MS-KNS) kinupristindalfopristin direnci 1(%5) suşta tespit edildi. MSSA ve MRSA suşlarının hiçbirinde kinupristin-dalfopristin direnci tespit edilmedi. Suşların tamamının vankomisine duyarlı olduğu bulundu. Stafilokok suşlarının kinupristindalfopristine yüksek oranda duyarlı oldukları bulundu. Sonuç olarak vankomisin gibi kinupristin-dalfopristin kombinasyonun da özellikle dirençli Gram-pozitif kok enfeksiyonlarının tedavisinde alternatif olabileceğini düşünmekteyiz.

Article Details

IN VITRO ACTIVITY OF QUINUPRISTIN-DALFOPRISTIN, METHICILLIN AND VANCOMYCIN AGAINST STAPHYLOCOCCUS STRAINS ISOLATED FROM CLINICAL SAMPLES

Authors: Erkan YULA | Turkan TOKA ÖZER | Ozcan DEVECİ | Alicem TEKİN | Keramettin YANIK | Suleyman DURMAZ

Publisher: Erciyes University

Journal: Journal of Health Sciences 2012 Vol.21 Issue 1, pp.45-49

Document Type: Article

Publication Date: 2/2012

Document Language: Turkish

Document ID (Org No.): 2000614358

Frequency: Tri-annual

pISSN: 1018-3655

Issues

Authors:

Erkan YULA | Turkan TOKA ÖZER | Ozcan DEVECİ | Alicem TEKİN | Keramettin YANIK | Suleyman DURMAZ

Publisher:

Erciyes University

Journal:

Journal of Health Sciences 2012 Vol.21 Issue 1, pp.45-49

Document Type:

Article

Publication Date:

2/2012

Document Language:

Turkish

Document ID (Org No.):

2000614358

Frequency:

Tri-annual

pISSN:

1018-3655