Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification

Junhuan Yang; Md. Ahsanul Kabir Khan; Sergio López-García; Shaista Nosheen; Yusuf Nazir; Huaiyuan Zhang; Victoriano Garre; Yuanda Song

doi:10.3844/ajbbsp.2020.138.147

Article Details

Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification

Authors:

Junhuan Yang | Md. Ahsanul Kabir Khan | Sergio López-García | Shaista Nosheen | Yusuf Nazir | Huaiyuan Zhang | Victoriano Garre | Yuanda Song

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.16 Issue 1, pp.138-147

Document Type:

Article

Publication Date:

3/2020

Document Language:

English

Document ID (Org No.):

2001397352

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2020.138.147

Keywords:

Abstract:

 Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA.

Article Details

Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification

Authors:

Junhuan Yang | Md. Ahsanul Kabir Khan | Sergio López-García | Shaista Nosheen | Yusuf Nazir | Huaiyuan Zhang | Victoriano Garre | Yuanda Song

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.16 Issue 1, pp.138-147

Document Type:

Article

Publication Date:

3/2020

Document Language:

English

Document ID (Org No.):

2001397352

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2020.138.147

Fatty Acid Desaturase 3

SDA Production

Homologous Overexpression

Mucor circinelloides

Issues

Article Details

Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification

Authors: Junhuan Yang | Md. Ahsanul Kabir Khan | Sergio López-García | Shaista Nosheen | Yusuf Nazir | Huaiyuan Zhang | Victoriano Garre | Yuanda Song

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.16 Issue 1, pp.138-147

Document Type: Article

Publication Date: 3/2020

Document Language: English

Document ID (Org No.): 2001397352

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2020.138.147

Fatty Acid Desaturase 3

SDA Production

Homologous Overexpression

Mucor circinelloides

Issues

Authors:

Junhuan Yang | Md. Ahsanul Kabir Khan | Sergio López-García | Shaista Nosheen | Yusuf Nazir | Huaiyuan Zhang | Victoriano Garre | Yuanda Song

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.16 Issue 1, pp.138-147

Document Type:

Article

Publication Date:

3/2020

Document Language:

English

Document ID (Org No.):

2001397352

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2020.138.147