A Comparison Between Three Serological Tests for  Brucella melitensis Infection in Sheep

Nizar ABUHARFEIL

Article Details

A Comparison Between Three Serological Tests for Brucella melitensis Infection in Sheep

Authors:

Nizar ABUHARFEIL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 1998 Vol.22 Issue 2, pp.119-122

Document Type:

Article

Publication Date:

1/1998

Document Language:

Turkish

Document ID (Org No.):

2000772626

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

A comparative study of the Rose Bengal test (RBT), Enzyme-Linked Immunosorbent Assay (ELISA) and complement fixation  test (CFT) was performed to analyze sera of two Awassi flocks. Flock A was composed of 215 ewes, naturally infected with  brucellosis. None of the animals were vaccinated. Flock B consisted of 336 non-infected non-vaccinated ewes. These served as nega-tive  controls. The two flocks were each divided into 4 groups, and samples were collected from one group every 6 months and subjected  to bacteriologic and serologic testing. Samples from any aborted ewes were also collected immediately after abortion and 6  weeks later. During the 2 year period of study, 57 ewes spontaneously aborted, out of which Brucella melitensis was isolated from  16 aborted fetuses. Blood from only 2 pregnant ewes were cultured positive to B. melitensis from flock A, and none from flock B.  RBT was demonstrated as the most suitable screening method in the field if suspected weak positive (+1) readings are considered  negative. ELISA is the most specific and sensitive method since no false negative results were recorded which is in contrast to other  methods. One single test is not sufficient to confirm the diagnosis of brucellosis and the combination of two tests should be performed.  These are preferably the RBT and ELISA.

 

A comparative study of the Rose Bengal test (RBT), Enzyme-Linked Immunosorbent Assay (ELISA) and complement fixation  test (CFT) was performed to analyze sera of two Awassi flocks. Flock A was composed of 215 ewes, naturally infected with  brucellosis. None of the animals were vaccinated. Flock B consisted of 336 non-infected non-vaccinated ewes. These served as nega-tive  controls. The two flocks were each divided into 4 groups, and samples were collected from one group every 6 months and subjected  to bacteriologic and serologic testing. Samples from any aborted ewes were also collected immediately after abortion and 6  weeks later. During the 2 year period of study, 57 ewes spontaneously aborted, out of which Brucella melitensis was isolated from  16 aborted fetuses. Blood from only 2 pregnant ewes were cultured positive to B. melitensis from flock A, and none from flock B.  RBT was demonstrated as the most suitable screening method in the field if suspected weak positive (+1) readings are considered  negative. ELISA is the most specific and sensitive method since no false negative results were recorded which is in contrast to other  methods. One single test is not sufficient to confirm the diagnosis of brucellosis and the combination of two tests should be performed.  These are preferably the RBT and ELISA.

Article Details

A Comparison Between Three Serological Tests for Brucella melitensis Infection in Sheep

Authors:

Nizar ABUHARFEIL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 1998 Vol.22 Issue 2, pp.119-122

Document Type:

Article

Publication Date:

1/1998

Document Language:

Turkish

Document ID (Org No.):

2000772626

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Sheep

brucellosis

ELISA

RBPT

CFT

Issues

Article Details

A Comparison Between Three Serological Tests for Brucella melitensis Infection in Sheep

Authors: Nizar ABUHARFEIL

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 1998 Vol.22 Issue 2, pp.119-122

Document Type: Article

Publication Date: 1/1998

Document Language: Turkish

Document ID (Org No.): 2000772626

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Sheep

brucellosis

ELISA

RBPT

CFT

Issues

Authors:

Nizar ABUHARFEIL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 1998 Vol.22 Issue 2, pp.119-122

Document Type:

Article

Publication Date:

1/1998

Document Language:

Turkish

Document ID (Org No.):

2000772626

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128