Utilizing Marker-Assisted Selection (MAS) for Morphological Traits in the F3 Generation of Tomato (Solanum lycopersicum).

Amani  Emsaed; Aysam M.  Fayed; Khaled  Elmeer

Article Details

Utilizing Marker-Assisted Selection (MAS) for Morphological Traits in the F3 Generation of Tomato (Solanum lycopersicum).

Authors:

Amani Emsaed | Aysam M. Fayed | Khaled Elmeer

Publisher:

University of Tripoli, Libya

Main Topics:

Marker-assisted | MAS | Molecular breeding | RAPD | Solanum lycopersicum and Tomato hybrid

Journal:

The Libyan Journal of Agriculture Vol.30 Issue 1, pp.59-68

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001535552

pISSN:

2708-6127

Keywords:

Abstract:

 The use of molecular markers has become a pivotal component of molecular breeding programs in tomato. In the third generation of this research, two lines derived from the crossbreeding of commercial hybrids were subjected to detailed analysis using molecular markers by ten RAPD-based primers. Among these primers, six (OPA01, OPA05, OPA07, OPA08, OPA09, and OPB10) successfully amplified targeted DNA regions. Significantly, primer OPA07 uniquely linked specific traits in the first line to distinct bands of 280 and 180 base pairs. In the second line, all six primers achieved amplification, with four primers (OPA07, OPA08, OPA09, and OPB10) associating selected traits with distinct bands of 450, 500, 350, and 450 base pairs, respectively. These bands are likely to serve as marker-assisted selection (MAS) tools, facilitating the selection of plants with desired phenotypic traits in tomato breeding. These markers are expected to greatly improve marker-assisted selection techniques and potentially reveal novel genetic resources, thus enriching the genetic diversity within tomato breeding programs for future advancements.

Article Details

Utilizing Marker-Assisted Selection (MAS) for Morphological Traits in the F3 Generation of Tomato (Solanum lycopersicum).

Authors: Amani Emsaed | Aysam M. Fayed | Khaled Elmeer

Publisher: University of Tripoli, Libya

Main Topics: Marker-assisted | MAS | Molecular breeding | RAPD | Solanum lycopersicum and Tomato hybrid

Journal: The Libyan Journal of Agriculture Vol.30 Issue 1, pp.59-68

Document Type: Article

Publication Date: 1/2025

Document Language: English

Document ID (Org No.): 2001535552

pISSN: 2708-6127

Marker-assisted

MAS

Molecular breeding

RAPD

Solanum lycopersicum and Tomato hybrid

Issues

Authors:

Amani Emsaed | Aysam M. Fayed | Khaled Elmeer

Publisher:

University of Tripoli, Libya

Main Topics:

Marker-assisted | MAS | Molecular breeding | RAPD | Solanum lycopersicum and Tomato hybrid

Journal:

The Libyan Journal of Agriculture Vol.30 Issue 1, pp.59-68

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001535552

pISSN:

2708-6127