Determination of Acute Effects of 2,4-Dichlorophenoxyacetic Acid on Antioxidant Enzymes and Lipid Peroxidation Levels in Zebrafish (Danio rerio Hamilton, 1822) Gills

Figen Esin KAYHAN; Güllü KAYMAK; Cansu Akbulut; Nazan Deniz YÖN ERTUĞ

doi:https://doi.org/10.23902/trkjnat.294722

Article Details

Determination of Acute Effects of 2,4-Dichlorophenoxyacetic Acid on Antioxidant Enzymes and Lipid Peroxidation Levels in Zebrafish (Danio rerio Hamilton, 1822) Gills

Authors:

Figen Esin KAYHAN | Güllü KAYMAK | Cansu Akbulut | Nazan Deniz YÖN ERTUĞ

Publisher:

Trakya University

Main Topics:

Life sciences

Journal:

Trakya University Journal of Natural Sciences 2017 Vol.18 Issue 2, pp.143-148

Document Type:

Article

Publication Date:

10/2017

Document Language:

English

Document ID (Org No.):

2000679187

Frequency:

Bi-Annual

eISSN:

2528-9691

DOI:

https://doi.org/10.23902/trkjnat.294722

Abstract:

 


The
aim of this study is to determine the potential of 2,4-D
(2,4-Dichlorophenoxyacetic acid) herbicide in causing acute oxidative stress in
the gills of zebrafish (Danio rerio
Hamilton, 1822) using spectrophotometric methods. Test animals except the
control group (Group-K) were exposed to sublethal doses (0.1ppm, 0.5ppm and
1ppm) of 2,4-D herbicide for 96 hours. Malondialdehyde (MDA) was used to
determine lipid peroxidation levels, and reduced glutathione (GSH), catalase
enzyme activity (CAT) and total protein (TP) levels were determined to
determine their effects on antioxidant systems in gill tissues. In this study,
total protein levels in gills of zebrafish exposed to sublethal doses of 2,4-D
were observed to be reduced compared to the control group. MDA levels
significantly increased compared to the control group. GSH levels increased in
the highest dose of 2,4-D herbicide but decreased in other doses. No
significant difference was found in CAT activity compared to the control group.

.

 


Bu
çalışmanın amacı; 2-4-D (2,4-Diklorofenoksiasetik asit) herbisitinin, zebra
balığı (Danio rerio Hamilton, 1822)
solungaçlarında akut oksidatif strese neden olma potansiyellerinin
spektrofotometrik yöntemlerle belirlenmesidir. Kontrol grubu (Grup-K) dışındaki
balıklar, 96 saat süreyle 2-4-D herbisitinin subletal dozlarının (0.1, 0.5 ve 1
ppm) etkisine bırakılmıştır. Solungaç dokusunda lipid peroksidasyon
seviyelerinin belirlenmesi amacıyla malondialdehit (MDA), antioksidan
sistemlere etkisini belirlemek amacıyla da indirgenmiş glutatyon (GSH), katalaz
enzim aktivitesi (CAT) ve total protein (TP) seviyeleri spektrofotometrik
yöntemler kullanılarak belirlenmiştir. Bu çalışmada 2,4-D’nin subletal
dozlarına maruz bırakılan zebra balıklarının solungaçlarında total protein seviyelerinin
kontrol grubuna oranla azaldığı gözlenmiştir. MDA seviyeleri kontrol grubuna
oranla önemli ölçüde artmıştır. GSH seviyeleri 2-4-D herbisitinin en yüksek
dozunda artarken diğer gruplarda azalmıştır. CAT aktivitesinde ise kontrol
grubuna oranla önemli bir fark gözlenmemiştir. 

.

Article Details

Determination of Acute Effects of 2,4-Dichlorophenoxyacetic Acid on Antioxidant Enzymes and Lipid Peroxidation Levels in Zebrafish (Danio rerio Hamilton, 1822) Gills

Authors: Figen Esin KAYHAN | Güllü KAYMAK | Cansu Akbulut | Nazan Deniz YÖN ERTUĞ

Publisher: Trakya University

Main Topics: Life sciences

Journal: Trakya University Journal of Natural Sciences 2017 Vol.18 Issue 2, pp.143-148

Document Type: Article

Publication Date: 10/2017

Document Language: English

Document ID (Org No.): 2000679187

Frequency: Bi-Annual

eISSN: 2528-9691

DOI: https://doi.org/10.23902/trkjnat.294722

Issues

Authors:

Figen Esin KAYHAN | Güllü KAYMAK | Cansu Akbulut | Nazan Deniz YÖN ERTUĞ

Publisher:

Trakya University

Main Topics:

Life sciences

Journal:

Trakya University Journal of Natural Sciences 2017 Vol.18 Issue 2, pp.143-148

Document Type:

Article

Publication Date:

10/2017

Document Language:

English

Document ID (Org No.):

2000679187

Frequency:

Bi-Annual

eISSN:

2528-9691

DOI:

https://doi.org/10.23902/trkjnat.294722