Reno-protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury

Fazile Nur Ekinci Akdemir; Ayhan Tanyeli; Ersen Eraslan; Mustafa Can Güler; Ömer Topdağı; Tuncer Nacar

Article Details

Reno-protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury

Authors:

Fazile Nur Ekinci Akdemir | Ayhan Tanyeli | Ersen Eraslan | Mustafa Can Güler | Ömer Topdağı | Tuncer Nacar

Publisher:

Akdeniz University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Akdeniz Tıp Dergisi Vol.9 Issue 2, pp.136-141

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001281421

pISSN:

2687-2781

Keywords:

Abstract:

 Purpose: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion RIR method. Material and Method: In the existing study, experimental animals were randomly assigned to 4 groups as sham, renal ischemia reperfusion RIR, DMSO and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion IR model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0,3 ml, 1 DMSO was administered intraperitoneally for each rat once in two 2 days for 10 days and a last dose was applied 30 minutes before reperfusion. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0,5 mgml for each rat every second days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated. Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatolIR group due to brusatol therapy compared to sham and DMSO groups. Conclusion: Consequently, brusatol demonstrated protective effects against RIR induced oxidative kidney injury in rats.

 Purpose: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion RIR method. Material and Method: In the existing study, experimental animals were randomly assigned to 4 groups as sham, renal ischemia reperfusion RIR, DMSO and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion IR model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0,3 ml, 1 DMSO was administered intraperitoneally for each rat once in two 2 days for 10 days and a last dose was applied 30 minutes before reperfusion. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0,5 mgml for each rat every second days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated. Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatolIR group due to brusatol therapy compared to sham and DMSO groups. Conclusion: Consequently, brusatol demonstrated protective effects against RIR induced oxidative kidney injury in rats.

 Purpose: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion RIR method. Material and Method: In the existing study, experimental animals were randomly assigned to 4 groups as sham, renal ischemia reperfusion RIR, DMSO and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion IR model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0,3 ml, 1 DMSO was administered intraperitoneally for each rat once in two 2 days for 10 days and a last dose was applied 30 minutes before reperfusion. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0,5 mgml for each rat every second days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated. Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatolIR group due to brusatol therapy compared to sham and DMSO groups. Conclusion: Consequently, brusatol demonstrated protective effects against RIR induced oxidative kidney injury in rats.

 Amaç: Bu çalışmanın amacı brusatol tedavisinin bilateral renal iskemi reperfüzyon RIR yöntemi ile indüklenen oksidatif böbrek hasarı üzerindeki potansiyel yararlı etkilerini belirlemektir. Gereç ve Yöntem: Mevcut çalışmada, deney hayvanları rastgele sham, renal iskemi reperfüzyonu RIR, DMSO ve brusatol grupları olarak 4 gruba ayrıldı. Sham grubu; arka bölge insizyon ile açıldı ve sonra sütüre edildi ancak iskemi reperfüzyon IR modeli oluşturulmadı. RIR grubunda 24 saatlik reperfüzyonu takiben 1 saatlik iskemi oluştu. DMSO grubunda, 10 gün boyunca iki günde bir kez her sıçan için 0,3 ml 1 DMSO intraperitonal olarak uygulandı ve reperfüzyondan 30 dakika önce son bir doz uygulandı. Daha sonra RIR grubunda anlatıldığı gibi IR modeli uygulandı. Brusatol grubunda brusatol, deneyden önce 10 gün boyunca her bir sıçan için 0,5 mgml olarak intraperitonal olarak uygulandı. Son doz reperfüzyondan 30 dakika önce uygulandı ve IR, RIR grubunda gösterildiği gibi gerçekleştirildi. Reperfüzyon döneminden sonra sıçanlar hareketsiz hale getirildi ve böbrek dokuları izole edildi. Bulgular: TNF-a, MDA ve IL-1β düzeyleri, OSI, TOS ve MPO değerleri anlamlı olarak yükselmiş, ancak TAS ve SOD düzeyleri sham grubuna göre RIR ve DMSO gruplarında azalmıştır. Öte yandan TAS ve SOD artarken OSI ve TOS değerleri, MPO ve TNF-α, MDA ve IL-1β düzeylerinin brusatol  IR grubunda sham ve DMSO gruplarına göre brusatol tedavisine bağlı olarak anlamlı düzeyde azaldığı görülmüştür. Sonuç: Sonuç olarak, brusatol sıçanlarda RIR kaynaklı oksidatif böbrek hasarına karşı koruyucu etkiler göstermiştir.

Article Details

Reno-protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury

Authors:

Fazile Nur Ekinci Akdemir | Ayhan Tanyeli | Ersen Eraslan | Mustafa Can Güler | Ömer Topdağı | Tuncer Nacar

Publisher:

Akdeniz University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Akdeniz Tıp Dergisi Vol.9 Issue 2, pp.136-141

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001281421

pISSN:

2687-2781

Brusatol

Renal Ischemia Reperfusion

Oxidative stress

Rat.

Brusatol

Renal İskemi Reperfüzyonu

Oksidatif stres

Sıçan.

Issues

Article Details

Authors: Fazile Nur Ekinci Akdemir | Ayhan Tanyeli | Ersen Eraslan | Mustafa Can Güler | Ömer Topdağı | Tuncer Nacar

Publisher: Akdeniz University

Main Topics: Klinik Tıp Bilimleri

Journal: Akdeniz Tıp Dergisi Vol.9 Issue 2, pp.136-141

Document Type: Article

Publication Date: 1/2023

Document Language: English

Document ID (Org No.): 2001281421

pISSN: 2687-2781

Issues

Authors:

Fazile Nur Ekinci Akdemir | Ayhan Tanyeli | Ersen Eraslan | Mustafa Can Güler | Ömer Topdağı | Tuncer Nacar

Publisher:

Akdeniz University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Akdeniz Tıp Dergisi Vol.9 Issue 2, pp.136-141

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001281421

pISSN:

2687-2781