LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

N. Akev*, A. Can* R. YANARDAĞ**

Article Details

LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Authors:

N. Akev, A. Can R. YANARDAĞ**

Publisher:

Istanbul University

Main Topics:

Pharmacology and therapeutics

Journal:

Journal of Faculty of Pharmacy of Istanbul University 1995 Vol.31 Issue 0, pp.1-10

Document Type:

Article

Publication Date:

9/2015

Document Language:

English

Document ID (Org No.):

2000513623

Frequency:

Monthly

eISSN:

2587-2087

Abstract:

 

Three electrophoretically distinct aspartate minotransferase isoenzymes
named AAT-1, AAT-2and AAT-3, were separated from lentil (Lens culinaris
Medik.) seeds by extraction, ammonium sulphate precipitation, hydroxylapatite
and DEAE-cellulose column chromatographies. AAT-1 was purified 228,
AAT-2,42 and AAT-3, 3.8 fold. The isoenzymes were examined by means of
polyacrylamide gel electrophoresis.Owing to its key role in general metabolism, aspartate adotransferase (AAT, E.C. 2.6.1.1.) which was formerly called glutamic oxaloacetic transaminase (GOT), occurs widely in nature. In animals, cytbsolic and rnitochon+al forms of AAT have been characterized and purified (1-4). AAT has also been studied in various plants (5, 6) and different isoeozyrnic forms have been r&-, ported. The enzyme has been extensively characterized in wheat germ (7,8), cauliflower (9), cotton seeds (lo), pea (ll), soybean (12-14), oat (15), lupine (16) and alfalfa (17,18).

 


Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jelelektroforezi ile incelendi.

Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jelelektroforezi ile incelendi.

.

Article Details

LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Authors: N. Akev*, A. Can* R. YANARDAĞ**

Publisher: Istanbul University

Main Topics: Pharmacology and therapeutics

Journal: Journal of Faculty of Pharmacy of Istanbul University 1995 Vol.31 Issue 0, pp.1-10

Document Type: Article

Publication Date: 9/2015

Document Language: English

Document ID (Org No.): 2000513623

Frequency: Monthly

eISSN: 2587-2087

Issues

Authors:

N. Akev, A. Can R. YANARDAĞ**

Publisher:

Istanbul University

Main Topics:

Pharmacology and therapeutics

Journal:

Journal of Faculty of Pharmacy of Istanbul University 1995 Vol.31 Issue 0, pp.1-10

Document Type:

Article

Publication Date:

9/2015

Document Language:

English

Document ID (Org No.):

2000513623

Frequency:

Monthly

eISSN:

2587-2087