Expression Analysis of DEK, AF4 and FLI1 Genes in All-Trans-Retinoic Acid (ATRA) Treated Acute Promyelocytic Leukaemia t(15;17) Patients by Quantitative Real-Time PCR

Hakan SAVLI; Sema SIRMA; Balint NAGY; Melih AKTAN; Günçağ DİNÇOL; Zafer SALCIOĞLU; Uğur ÖZBEK

Article Details

Expression Analysis of DEK, AF4 and FLI1 Genes in All-Trans-Retinoic Acid (ATRA) Treated Acute Promyelocytic Leukaemia t(15;17) Patients by Quantitative Real-Time PCR

Authors:

Hakan SAVLI | Sema SIRMA | Balint NAGY | Melih AKTAN | Günçağ DİNÇOL | Zafer SALCIOĞLU | Uğur ÖZBEK

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2004 Vol.34 Issue 2, pp.85-89

Document Type:

Article

Publication Date:

1/2004

Document Language:

Turkish

Document ID (Org No.):

2000756608

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

All-trans retinoic acid (ATRA) sensitivity of acute promyelocytic leukaemia (APL) cells is strictly dependent on the presence of t(15;17), but the molecular background of this sensitivity remains obscure. We showed the down- regulation of DEK, AF4 and FLI1 genes in the vitamin D treatment of APL cell line HL-60 , using cDNA array technology in our previous study. This finding prompted us to investigate the expression of these genes in APL patient samples. The effect of ATRA was studied in 6 APL patients carrying t(15;17). Two samples from each patient were compared with a primary diagnostic sample and a sample taken at remission. Frozen RNA samples were obtained from bone marrow aspirates and converted to cDNA, and then quantitative real- time PCR was performed. Among the traits of these 3 genes, the over-expression of FLI1 was particularly remarkable. The findings suggest that FLI1 over expression may be involved in APL and that it can be corrected after remission induction. Whether or not ATRA treatment has any effect on these genes may be studied in an experimental model in order to find new potential targets for rational drug discoveries.

 

All-trans retinoic acid (ATRA) sensitivity of acute promyelocytic leukaemia (APL) cells is strictly dependent on the presence of t(15;17), but the molecular background of this sensitivity remains obscure. We showed the down- regulation of DEK, AF4 and FLI1 genes in the vitamin D treatment of APL cell line HL-60 , using cDNA array technology in our previous study. This finding prompted us to investigate the expression of these genes in APL patient samples. The effect of ATRA was studied in 6 APL patients carrying t(15;17). Two samples from each patient were compared with a primary diagnostic sample and a sample taken at remission. Frozen RNA samples were obtained from bone marrow aspirates and converted to cDNA, and then quantitative real- time PCR was performed. Among the traits of these 3 genes, the over-expression of FLI1 was particularly remarkable. The findings suggest that FLI1 over expression may be involved in APL and that it can be corrected after remission induction. Whether or not ATRA treatment has any effect on these genes may be studied in an experimental model in order to find new potential targets for rational drug discoveries.

Article Details

Expression Analysis of DEK, AF4 and FLI1 Genes in All-Trans-Retinoic Acid (ATRA) Treated Acute Promyelocytic Leukaemia t(15;17) Patients by Quantitative Real-Time PCR

Authors:

Hakan SAVLI | Sema SIRMA | Balint NAGY | Melih AKTAN | Günçağ DİNÇOL | Zafer SALCIOĞLU | Uğur ÖZBEK

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2004 Vol.34 Issue 2, pp.85-89

Document Type:

Article

Publication Date:

1/2004

Document Language:

Turkish

Document ID (Org No.):

2000756608

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

DEK

AF4

FLI1

Acute Promyelocytic Leukemia

All-Trans-Retimic Acid

Issues

Article Details

Expression Analysis of DEK, AF4 and FLI1 Genes in All-Trans-Retinoic Acid (ATRA) Treated Acute Promyelocytic Leukaemia t(15;17) Patients by Quantitative Real-Time PCR

Authors: Hakan SAVLI | Sema SIRMA | Balint NAGY | Melih AKTAN | Günçağ DİNÇOL | Zafer SALCIOĞLU | Uğur ÖZBEK

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2004 Vol.34 Issue 2, pp.85-89

Document Type: Article

Publication Date: 1/2004

Document Language: Turkish

Document ID (Org No.): 2000756608

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

DEK

AF4

FLI1

Acute Promyelocytic Leukemia

All-Trans-Retimic Acid

Issues

Authors:

Hakan SAVLI | Sema SIRMA | Balint NAGY | Melih AKTAN | Günçağ DİNÇOL | Zafer SALCIOĞLU | Uğur ÖZBEK

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2004 Vol.34 Issue 2, pp.85-89

Document Type:

Article

Publication Date:

1/2004

Document Language:

Turkish

Document ID (Org No.):

2000756608

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144