Extended Spectrum Β-Lactamase Producing Escherichia Coli in Clinical Isolates in Benghazi , Libya : Phenotypic Detection and Antimicrobial Susceptibility Pattern

Altayar , Marei A.; Thokar , Manzoor A.; Mohammad , Mohammad A.

Article Details

Extended Spectrum Β-Lactamase Producing Escherichia Coli in Clinical Isolates in Benghazi , Libya : Phenotypic Detection and Antimicrobial Susceptibility Pattern

Authors:

Altayar , Marei A. | Thokar , Manzoor A. | Mohammad , Mohammad A.

Publisher:

Islamic World Academy of Sciences

Main Topics:

Medicine

Journal:

Medical Journal of Islamic World Academy of Sciences 2012 vol.20 Issue 2, pp.49-56

Document Type:

Article

Publication Date:

4/2012

Document Language:

English

Document ID (Org No.):

21457

Frequency:

Quarterly

pISSN:

10163360

Descriptors:

Keywords:

Abstract:

 β-lactams are the most widely used group of antimicrobials; however, growing resistance tothese invaluable drugs mediated by extended spectrum β-lactamase (ESBL) enzymes is a major concern. Thepresent study was undertaken to determine the prevalence of these enzymes and their effect on antimicrobial susceptibilitypattern by different phenotypic detection tests in clinical isolates of Escherichia coli in Benghazi,Libya. Antimicrobial susceptibility testing was carried out by Kirby-Bauer method. Ceftazidime and cefotaximewere used for screening potential ESBL producers. Confirmation was done by a combination of double disk synergytest (DDST) and phenotypic confirmatory disk diffusion tests (PCDDTs).A total of 120 E. coli strains (40 urine, 20 sputum, 20 blood, and 40 wound swabs) from inpatients at differenthospitals of Benghazi, Libya, were included in the study, of which, 24 (20%) isolates were ESBL producers.The resistance pattern to the tested antibiotics was as follows: ampicillin (80% ), co-trimoxazole (60%),ciprofloxacine (40%), cefotaxime (30%), ceftazidime (30%),Ceftriaxone (30%), gentamicin (30%), cefpirome (35%), ofloxacin (30%), imipenem (25%), and nitrofurantoin(40%).All the isolates tested showed resistance to two or more drugs and were considered to be multidrug resistant.A higher rate of ESBL production and multidrug resistance was seen among isolates from pus swabs ascompared to other sources. ESBL producers mediated high resistance to both β-lactams and non-β-lactams. Prolongedhospital stay and prior use of third-generation cephalosporins were identified as important risk factorsfor ESBL acquisition.There is insufficient data regarding ESBL prevalence among E. coli strains from Benghazi, Libya. ESBLsnot only pose a great threat to future of β-lactams, but they also endanger the utility of many non-β-lactams.Toensure rationale in antibiotic treatment, ESBL detection and reporting assumes a priority in near future in Benghazi,Libya.

Article Details

Extended Spectrum Β-Lactamase Producing Escherichia Coli in Clinical Isolates in Benghazi , Libya : Phenotypic Detection and Antimicrobial Susceptibility Pattern

Authors:

Altayar , Marei A. | Thokar , Manzoor A. | Mohammad , Mohammad A.

Publisher:

Islamic World Academy of Sciences

Main Topics:

Medicine

Journal:

Medical Journal of Islamic World Academy of Sciences 2012 vol.20 Issue 2, pp.49-56

Document Type:

Article

Publication Date:

4/2012

Document Language:

English

Document ID (Org No.):

21457

Frequency:

Quarterly

pISSN:

10163360

Descriptors:

ENTERIC DISEASES

COLITIS

PATHOGENS

ANTIBIOTICS

ENTERITIS

ESCHERICHIA

LIBYA

Escherichia coli

multidrug resistance

extended spectrum β-lactamase (ESBL)

Issues

Article Details

Authors: Altayar , Marei A. | Thokar , Manzoor A. | Mohammad , Mohammad A.

Publisher: Islamic World Academy of Sciences

Main Topics: Medicine

Journal: Medical Journal of Islamic World Academy of Sciences 2012 vol.20 Issue 2, pp.49-56

Document Type: Article

Publication Date: 4/2012

Document Language: English

Document ID (Org No.): 21457

Frequency: Quarterly

pISSN: 10163360

Descriptors:

Issues

Authors:

Altayar , Marei A. | Thokar , Manzoor A. | Mohammad , Mohammad A.

Publisher:

Islamic World Academy of Sciences

Main Topics:

Medicine

Journal:

Medical Journal of Islamic World Academy of Sciences 2012 vol.20 Issue 2, pp.49-56

Document Type:

Article

Publication Date:

4/2012

Document Language:

English

Document ID (Org No.):

21457

Frequency:

Quarterly

pISSN:

10163360