Assessment of IL-12 and Liver Enzyme Levels in Serum of Iraqi Patients with Hepatitis B Virus

Nassif Nada Najm; Mohanad Hasan Mahmood Al-Izzi

Article Details

Assessment of IL-12 and Liver Enzyme Levels in Serum of Iraqi Patients with Hepatitis B Virus

Authors:

Nassif Nada Najm | Mohanad Hasan Mahmood Al-Izzi

Publisher:

Tikrit University, Iraq

Main Topics:

Hepatitis B | Interleukin-12 | Alanine Aminotransferase | Total Serum Bilirubin

Journal:

Tikrit Journal of Pharmaceutical Sciences Vol.19 Issue 1, pp.65-75

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001532184

pISSN:

2664-231X

Keywords:

Abstract:

 Background: Chronic Hepatitis B virus (HBV) infection leads to liver dysfunction through persistent inflammation and immune dysregulation. Cytokines such as interleukin-12 (IL-12) and liver enzymes including Alanine aminotransferase, Aspartate aminotransferase, Alkaline phosphatase, and Total serum bilirubin are critical markers for evaluating liver damage and immune activation.
Objective: This study is aimed to assess the levels of IL-12 and hepatic enzymes in patients with HBV to explore their correlation with liver injury and immune response.
Methods: A case-control study was conducted on 90 individuals (40 HBV patients and 50 healthy controls) in Kirkuk, Iraq. Serum IL-12 was quantified using a sandwich ELISA method (FineTest®, China; Cat. No: EH0565), and absorbance was measured using a BioTek® ELx800 microplate reader (Agilent Technologies, USA). Liver enzymes were analyzed via standard colorimetric assays.
Results: Patients with HBV exhibited significantly elevated interleukin-12 (IL-12) levels (4707.21 pg/mL) compared to controls (1855.26 pg/mL; p < 0.000001). Similarly, liver enzyme levels were markedly higher in HBV patients than in healthy controls: alanine aminotransferase (ALT) was 46.13 IU/L vs. 21.91 IU/L (p = 0.000013), aspartate aminotransferase (AST) was 47.75 IU/L vs. 22.08 IU/L (p = 0.000042), alkaline phosphatase (ALP) was 194.57 IU/L vs. 87.49 IU/L (p = 0.000036), and total serum bilirubin (TSB) was 1.61 mg/dL vs. 0.69 mg/dL (p = 0.000029).
Conclusion: Elevated IL-12 and liver enzyme levels suggest ongoing hepatic damage and immune dysregulation in HBV patients. These biomarkers may support early diagnosis and monitoring of disease progression in chronic HBV infection.

Article Details

Assessment of IL-12 and Liver Enzyme Levels in Serum of Iraqi Patients with Hepatitis B Virus

Authors: Nassif Nada Najm | Mohanad Hasan Mahmood Al-Izzi

Publisher: Tikrit University, Iraq

Main Topics: Hepatitis B | Interleukin-12 | Alanine Aminotransferase | Total Serum Bilirubin

Journal: Tikrit Journal of Pharmaceutical Sciences Vol.19 Issue 1, pp.65-75

Document Type: Article

Publication Date: 1/2025

Document Language: English

Document ID (Org No.): 2001532184

pISSN: 2664-231X

Hepatitis B

Interleukin-12

Alanine Aminotransferase

Total Serum Bilirubin

Issues

Authors:

Nassif Nada Najm | Mohanad Hasan Mahmood Al-Izzi

Publisher:

Tikrit University, Iraq

Main Topics:

Hepatitis B | Interleukin-12 | Alanine Aminotransferase | Total Serum Bilirubin

Journal:

Tikrit Journal of Pharmaceutical Sciences Vol.19 Issue 1, pp.65-75

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001532184

pISSN:

2664-231X