Expression of the Low-density Lipoprotein Receptor (LDLR) Gene Family in CD133+/CD44+ Prostate Cancer Stem Cells

Burak Cem Soner; Eda Açıkgöz; Fahriye Duzagac; Cuneyd Parlayan

Article Details

Expression of the Low-density Lipoprotein Receptor (LDLR) Gene Family in CD133+/CD44+ Prostate Cancer Stem Cells

Authors:

Burak Cem Soner | Eda Açıkgöz | Fahriye Duzagac | Cuneyd Parlayan

Publisher:

Dokuz Eylul University

Main Topics:

Health Care Administration

Journal:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.410-417

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283430

pISSN:

2564-7288

Keywords:

Abstract:

 Aim: The low-density lipoprotein receptor gene LDLR family plays a fundamental role in many malignancies and may have a putative cancer-boosting function. In our study, we have attempted to comparatively investigate the differential gene expressions of LDLR family in normal prostate epithelial cell line RWPE-1, prostate cancer cell line DU145 cell line, prostate cancer stem cells DU145 CSCs and non-CSCs DU145 non-CSCs, bulk population. Materials and Methods: Cancer stem cells in DU-145 prostate cancer cell line were isolated by flow cytometry according to CD133 and CD44 cell surface properties. Whole transcriptome sequencing data was comprehensively analyzed for each group. The protein-protein interaction network was determined using the STRING protein database. Results: Our data showed that the expression levels of LRP1, LRP3, LRP8 and LRP11 were increased in the DU145 CSCs relative to the normal prostate epithelial cell line. Conclusion: Overall, our data suggest that the LRP functions andor the expression in prostate cancer may ultimately change the invasive phenotype of the CSCs.

 Aim: The low-density lipoprotein receptor gene LDLR family plays a fundamental role in many malignancies and may have a putative cancer-boosting function. In our study, we have attempted to comparatively investigate the differential gene expressions of LDLR family in normal prostate epithelial cell line RWPE-1, prostate cancer cell line DU145 cell line, prostate cancer stem cells DU145 CSCs and non-CSCs DU145 non-CSCs, bulk population. Materials and Methods: Cancer stem cells in DU-145 prostate cancer cell line were isolated by flow cytometry according to CD133 and CD44 cell surface properties. Whole transcriptome sequencing data was comprehensively analyzed for each group. The protein-protein interaction network was determined using the STRING protein database. Results: Our data showed that the expression levels of LRP1, LRP3, LRP8 and LRP11 were increased in the DU145 CSCs relative to the normal prostate epithelial cell line. Conclusion: Overall, our data suggest that the LRP functions andor the expression in prostate cancer may ultimately change the invasive phenotype of the CSCs.

 Aim: The low-density lipoprotein receptor gene LDLR family plays a fundamental role in many malignancies and may have a putative cancer-boosting function. In our study, we have attempted to comparatively investigate the differential gene expressions of LDLR family in normal prostate epithelial cell line RWPE-1, prostate cancer cell line DU145 cell line, prostate cancer stem cells DU145 CSCs and non-CSCs DU145 non-CSCs, bulk population. Materials and Methods: Cancer stem cells in DU-145 prostate cancer cell line were isolated by flow cytometry according to CD133 and CD44 cell surface properties. Whole transcriptome sequencing data was comprehensively analyzed for each group. The protein-protein interaction network was determined using the STRING protein database. Results: Our data showed that the expression levels of LRP1, LRP3, LRP8 and LRP11 were increased in the DU145 CSCs relative to the normal prostate epithelial cell line. Conclusion: Overall, our data suggest that the LRP functions andor the expression in prostate cancer may ultimately change the invasive phenotype of the CSCs.

 Aim: The low-density lipoprotein receptor gene LDLR family plays a fundamental role in many malignancies and may have a putative cancer-boosting function. In our study, we have attempted to comparatively investigate the differential gene expressions of LDLR family in normal prostate epithelial cell line RWPE-1, prostate cancer cell line DU145 cell line, prostate cancer stem cells DU145 CSCs and non-CSCs DU145 non-CSCs, bulk population. Materials and Methods: Cancer stem cells in DU-145 prostate cancer cell line were isolated by flow cytometry according to CD133 and CD44 cell surface properties. Whole transcriptome sequencing data was comprehensively analyzed for each group. The protein-protein interaction network was determined using the STRING protein database. Results: Our data showed that the expression levels of LRP1, LRP3, LRP8 and LRP11 were increased in the DU145 CSCs relative to the normal prostate epithelial cell line. Conclusion: Overall, our data suggest that the LRP functions andor the expression in prostate cancer may ultimately change the invasive phenotype of the CSCs.

Article Details

Expression of the Low-density Lipoprotein Receptor (LDLR) Gene Family in CD133+/CD44+ Prostate Cancer Stem Cells

Authors:

Burak Cem Soner | Eda Açıkgöz | Fahriye Duzagac | Cuneyd Parlayan

Publisher:

Dokuz Eylul University

Main Topics:

Health Care Administration

Journal:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.410-417

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283430

pISSN:

2564-7288

LDLR gen family

cancer stem cell

prostate cancer

transcriptional regulators

Issues

Article Details

Expression of the Low-density Lipoprotein Receptor (LDLR) Gene Family in CD133+/CD44+ Prostate Cancer Stem Cells

Authors: Burak Cem Soner | Eda Açıkgöz | Fahriye Duzagac | Cuneyd Parlayan

Publisher: Dokuz Eylul University

Main Topics: Health Care Administration

Journal: Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.410-417

Document Type: Article

Publication Date: 1/2023

Document Language: English

Document ID (Org No.): 2001283430

pISSN: 2564-7288

LDLR gen family

cancer stem cell

prostate cancer

transcriptional regulators

Issues

Authors:

Burak Cem Soner | Eda Açıkgöz | Fahriye Duzagac | Cuneyd Parlayan

Publisher:

Dokuz Eylul University

Main Topics:

Health Care Administration

Journal:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.410-417

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283430

pISSN:

2564-7288