Determination of phenolic acids in pomegranate juices by HPLC-DAD

Mürüvvet KARAKAPLAN; Mustafa ÖZCAN

Article Details

Determination of phenolic acids in pomegranate juices by HPLC-DAD

Authors:

Mürüvvet KARAKAPLAN | Mustafa ÖZCAN

Publisher:

Osman SAĞDIÇ

Main Topics:

Applied physics

Journal:

European Journal of Science and Technology 2017 Vol.6 Issue 10, pp.32-37

Document Type:

Article

Publication Date:

7/2017

Document Language:

English

Document ID (Org No.):

2000920420

Frequency:

Quarterly

eISSN:

2148-2683

Keywords:

Abstract:

 













In this study, five
phenolic acids, gallic, chlorogenic, caffeic, p-coumaric and ferulic acid were
determined in freshly squeezed pomegranate juice, commercial pomegranate juice
and pomegranate juice concentrate beverages. 
For this purpose, an analytical method using high-performance liquid
chromatography (HPLC), with a combination of photodiode array detection (DAD),
was developed for the characterization and quantification of phenolic acid in
pomegranate juices. Then the analyses of pomegranate juices was performed. The
limit of detection (LOD, S/N=3) of individual compounds was
gallic acid 0.1 mg/L, chlorogenic acid 0.01 mg/L, caffeic acid 0.03mg/L,
p-coumaric acid 0.02 mg/L and ferulic acid 0.04 mg/L. The limit of quantitation
(LOQ, S/N=10) of individual compounds was gallic acid 0.4 mg/L,
chlorogenic acid 0.05 mg/L, caffeic acid 0.1 mg/L, p-coumaric acid 0.1 mg/L and
ferulic acid 0.1 mg/L. The relative standard deviation (% RSD) in commericial
pomegranate juice was found to be galli cacid % 0.08, chlorogenic acid % 0.32,
caffeic acid % 0.21, p-coumaric acid % 0.08 and ferulic acid % 0.38. The
relative standard deviation (% RSD) in freshly squeezed pomegranate juice was
found to be gallic acid % 0.01, chlorogenic acid % 0.46, caffeic acid % 0.17,
p-coumaric acid % 0.04 and ferulic acid % 0.8. The relative standard deviation
(% RSD) in pomegranate concentrate juice was found to be gallic acid % 0.78,
chlorogenic acid % 0.25, caffeic acid % 0.49, p-coumaric acid % 0.36 and
ferulic acid % 0.17.


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Bu çalışmada taze sıkılmış nar suyu, ticari nar suyu ve nar suyu konsantresi içeceklerinde beş fenolik asit, gallik, klorojenik, kafeik, p-kumarik ve ferulik asit tespit edilmiştir. Bu amaçla, nar suyu seralarda fenolik asidin karakterizasyonu ve kantifikasyonu için, yüksek performanslı sıvı kromatografisi (HPLC) kullanan, fotodiyot dizi tespiti (DAD) kombinasyonlu analitik bir yöntem geliştirildi. Sonra nar suyu analizleri yapıldı. Tek tek bileşiklerin saptama limiti, gallik asit 0.1 mg / L, klorojenik asit 0.01 mg / L, kafeik asit 0.03 mg / L, p-kumarik asit 0.02 mg / L ve ferulik asit 0.04'tür (LOD, S / N = mg / L'dir. Bireysel bileşiklerin nicelik sınırı 0.4 mg / L gallik asit, klorojenik asit 0.05 mg / L, kafeik asit 0.1 mg / L, p-kumar asit 0.1 mg / L ve ferulik asit 0.1'dir (LOQ, S / N = 10) mg / L'dir. Ticari nar suyu içindeki bağıl standart sapma (% RSD), galli k asit% 0.08, klorojenik asit% 0.32, kafeik asit% 0.21, p-kumar asit% 0.08 ve ferulik asit% 0.38 olarak bulundu. Taze sıkılmış nar suyundaki göreli standart sapma (% RSD), gallik asit% 0.01, klorojenik asit% 0.46, kafeik asit% 0.17, p-kumarik asit% 0.04 ve ferulik asit% 0.8 olarak bulundu. Nar konsantresi suyundaki göreli standart sapma (% RSD), gallik asit% 0.78, klorojenik asit% 0.25, kafeik asit% 0.49, p-kumar asit% 0.36 ve ferulik asit% 0.17 olarak bulundu.

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Article Details

Determination of phenolic acids in pomegranate juices by HPLC-DAD

Authors:

Mürüvvet KARAKAPLAN | Mustafa ÖZCAN

Publisher:

Osman SAĞDIÇ

Main Topics:

Applied physics

Journal:

European Journal of Science and Technology 2017 Vol.6 Issue 10, pp.32-37

Document Type:

Article

Publication Date:

7/2017

Document Language:

English

Document ID (Org No.):

2000920420

Frequency:

Quarterly

eISSN:

2148-2683

HPLC

Gallik asit

Klorojenik asit

kafeik asit

p-kumarik asit

ferulik asit

Issues

Article Details

Determination of phenolic acids in pomegranate juices by HPLC-DAD

Authors: Mürüvvet KARAKAPLAN | Mustafa ÖZCAN

Publisher: Osman SAĞDIÇ

Main Topics: Applied physics

Journal: European Journal of Science and Technology 2017 Vol.6 Issue 10, pp.32-37

Document Type: Article

Publication Date: 7/2017

Document Language: English

Document ID (Org No.): 2000920420

Frequency: Quarterly

eISSN: 2148-2683

HPLC

Gallik asit

Klorojenik asit

kafeik asit

p-kumarik asit

ferulik asit

Issues

Authors:

Mürüvvet KARAKAPLAN | Mustafa ÖZCAN

Publisher:

Osman SAĞDIÇ

Main Topics:

Applied physics

Journal:

European Journal of Science and Technology 2017 Vol.6 Issue 10, pp.32-37

Document Type:

Article

Publication Date:

7/2017

Document Language:

English

Document ID (Org No.):

2000920420

Frequency:

Quarterly

eISSN:

2148-2683