Effect of Effective Microorganisms on intestinal morphology and morphometry in  Japanese quails

Michał GESEK; Rajmund SOKÓŁ; Barry D. LAMBERT; İwona OTROCKA-DOMAGAŁA

Article Details

Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Authors:

Michał GESEK | Rajmund SOKÓŁ | Barry D. LAMBERT | İwona OTROCKA-DOMAGAŁA

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.285-291

Document Type:

Article

Publication Date:

8/2018

Document Language:

English

Document ID (Org No.):

2000764587

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Abstract:

 

The aim of this study was to determine the effect of supplementation with Effective Microorganisms (EM) on the morphology 
and the morphometry of the intestine, and on proliferation activity of crypt cells in Japanese quails. The EM group (16 birds) received 
EM  for  30  days  in  water  (3  L/1000  L  of  water)  and  in  feed  (5  kg/1000  kg  of  feed)  mixed  with  standard  diet.  The  second  group  (16  
birds)  was  established  as  the  control  group  (no  EM  supplementation).  Histopathological  analysis  revealed  infiltration  of  lymphoid  
cells mainly in the duodenum and jejunum similarly in the EM and the control groups. The EM group showed irregular villus surfaces 
when compared to the control group. Morphometric analysis revealed significant differences in the EM group for villus width in the 
duodenum (186.0 μm to 170.7 μm; P ≤ 0.05), in crypt depth (129.2 ?m to 110.7 μm; P ≤ 0.05), muscular layer thickness in the jejunum 
(70.88 ?m to 57.44 ?m) (P ≤ 0.05), and muscular layer thickness in the ileum (76.88 ?m to 64.76 ?m;
P ≤ 0.05). Immunohistochemical 
analysis  of  proliferating  cell  nuclear  antigen  activity  was  similar  for  the  EM  and  the  control  groups:  in  the  duodenum  90.82%  in  the  
control group and 91.89% in the EM group, in the jejunum 93.32% in the control group and 94.53% in the EM group, and in the ileum 
92.53% in the control group and 93.92% in the EM group. Supplementation with EM resulted in improved structure and functions of 
the villi and changed their surface, height, width, and crypt depth.

Article Details

Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Authors: Michał GESEK | Rajmund SOKÓŁ | Barry D. LAMBERT | İwona OTROCKA-DOMAGAŁA

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.285-291

Document Type: Article

Publication Date: 8/2018

Document Language: English

Document ID (Org No.): 2000764587

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Issues

Authors:

Michał GESEK | Rajmund SOKÓŁ | Barry D. LAMBERT | İwona OTROCKA-DOMAGAŁA

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.285-291

Document Type:

Article

Publication Date:

8/2018

Document Language:

English

Document ID (Org No.):

2000764587

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128