Influence of Zebularin on Antioxidant Enzymes and Lipid Peroxidation of Galleria mellonella (Lepidoptera: Pyralidae)

Ebru Çakır; Fevzi Uçkan; Ekrem Ergin; Rabia Özbek

Article Details

Influence of Zebularin on Antioxidant Enzymes and Lipid Peroxidation of Galleria mellonella (Lepidoptera: Pyralidae)

Authors:

Ebru Çakır | Fevzi Uçkan | Ekrem Ergin | Rabia Özbek

Publisher:

Mevlüt AKÇURA

Main Topics:

Agricultural | Veterinary and Food Sciences

Journal:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.457-464

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001288330

pISSN:

2148-3647

Keywords:

Abstract:

 Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to convert it to 5-methylcytosine. Zebularine ZEB reactivates hypermethylated genes in yeast and solid tumor cells, with DNA methyltransferases that show a preferential effect on cancer cells. We investigated the effects of various doses 0.25-32 mgml of ZEB, on antioxidant enzyme activity and lipid peroxidation in wax moth Galleria mellonella last instars. Injection of ZEB in last instars G. mellonella resulted in increases in SOD, MDA, and GST levels, in order of magnitude, but not in CAT enzyme activity. Two possible reasons were predicted for these results. This effect could be a result of the inhibitory effect of ZEB on DNA methylation in G. mellonella, which leaded to reducing gene transcription in insects. Or it was likely that high oxidative stress might cause a decreasing activity of enzymes.

 Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to convert it to 5-methylcytosine. Zebularine ZEB reactivates hypermethylated genes in yeast and solid tumor cells, with DNA methyltransferases that show a preferential effect on cancer cells. We investigated the effects of various doses 0.25-32 mgml of ZEB, on antioxidant enzyme activity and lipid peroxidation in wax moth Galleria mellonella last instars. Injection of ZEB in last instars G. mellonella resulted in increases in SOD, MDA, and GST levels, in order of magnitude, but not in CAT enzyme activity. Two possible reasons were predicted for these results. This effect could be a result of the inhibitory effect of ZEB on DNA methylation in G. mellonella, which leaded to reducing gene transcription in insects. Or it was likely that high oxidative stress might cause a decreasing activity of enzymes.

 Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to convert it to 5-methylcytosine. Zebularine ZEB reactivates hypermethylated genes in yeast and solid tumor cells, with DNA methyltransferases that show a preferential effect on cancer cells. We investigated the effects of various doses 0.25-32 mgml of ZEB, on antioxidant enzyme activity and lipid peroxidation in wax moth Galleria mellonella last instars. Injection of ZEB in last instars G. mellonella resulted in increases in SOD, MDA, and GST levels, in order of magnitude, but not in CAT enzyme activity. Two possible reasons were predicted for these results. This effect could be a result of the inhibitory effect of ZEB on DNA methylation in G. mellonella, which leaded to reducing gene transcription in insects. Or it was likely that high oxidative stress might cause a decreasing activity of enzymes.

 Sitozin metilasyonudaki sitozin kalıntısını 5-metilsitozine dönüştürmek için bir metil grubu ekleyen süreçtir. Metilasyon sürecine DNA metiltransferazları tarafından aracılık edilir. Zebularin ZEB, ur ve kist tümör hücrelerinde hipermetile edilmiş genleri yeniden aktive eder, kanser hücreleri üzerinde tercihli bir etki gösteren DNA metiltransferazları ile kovalent bir kompleks oluşturan sitidin analoğudur. Bal mumu güvesi Galleria mellonellada son evre larvalarına farklı dozlarda 0.25-32 mg  ml verilen ZEBin antioksidan enzim aktivitesi ve lipit peroksidasyonu üzerindeki etkileri incelendi. ZEB enjeksiyonu, son aşamada G. mellonella larvalarında CAT enzim aktivitesinde değil ama büyüklük sırasına göre SOD, MDA ve GST seviyesinde artışa neden oldu. Sonuçlar için iki olası sebep öngörülmektedir. Birincisi, ZEBin, G. mellonellada DNA metilasyonu üzerindeki engelleyici etkisinin bir sonucu olabilir, bu da böceklerde gen transkripsiyonunun azaltılmasına yol açabilmektedir. İkincisi, yüksek oksidatif stresin enzimlerin azalan aktivitesine neden olması muhtemeldir.

Article Details

Influence of Zebularin on Antioxidant Enzymes and Lipid Peroxidation of Galleria mellonella (Lepidoptera: Pyralidae)

Authors:

Ebru Çakır | Fevzi Uçkan | Ekrem Ergin | Rabia Özbek

Publisher:

Mevlüt AKÇURA

Main Topics:

Agricultural | Veterinary and Food Sciences

Journal:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.457-464

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001288330

pISSN:

2148-3647

Antioxidant enzymes

DNA methilation

Galleria mellonella

lipid peroxidation

zebularineantioksidan enzimleri

DNA metilasyonu

Galleria mellonella

zebularin

Lipit peroksidasyonu

Issues

Article Details

Authors: Ebru Çakır | Fevzi Uçkan | Ekrem Ergin | Rabia Özbek

Publisher: Mevlüt AKÇURA

Main Topics: Agricultural | Veterinary and Food Sciences

Journal: Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.457-464

Document Type: Article

Publication Date: 4/2023

Document Language: English

Document ID (Org No.): 2001288330

pISSN: 2148-3647

Issues

Authors:

Ebru Çakır | Fevzi Uçkan | Ekrem Ergin | Rabia Özbek

Publisher:

Mevlüt AKÇURA

Main Topics:

Agricultural | Veterinary and Food Sciences

Journal:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 2, pp.457-464

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001288330

pISSN:

2148-3647