HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Meltem Bozacı Kılıçoğlu; Baris Ertugrul; Göksu Kaşarcı; Şenol Çomoğlu; Levent Aydemir; Meryem Nesil Keles Türel; A Gelincik; Bedia Çakmakoğlu

Article Details

HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Authors:

Meltem Bozacı Kılıçoğlu | Baris Ertugrul | Göksu Kaşarcı | Şenol Çomoğlu | Levent Aydemir | Meryem Nesil Keles Türel | A Gelincik | Bedia Çakmakoğlu

Publisher:

Istanbul University

Main Topics:

Sağlık Kurumları Yönetimi

Journal:

İstanbul Tıp Fakültesi Dergisi Vol.86 Issue 2, pp.152-158

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001287779

pISSN:

1305-6441

Keywords:

Abstract:

 Objective: Chronic rhinosinusitis with nasal polyposis CRSwNP is a multifactorial disease in which the sinus and nasal mucosa are affected. We investigated the relationship between the genetic polymorphism of MMP9 rs17576 and TIMP1 rs48498 and serum levels, and we wanted to investigate the effect of MMP9 and TIMP1 genotypes on Vitamin serum levels in CRSwNP with and without Aspirin-Exacerbated Respiratory Disease AERD. Materials and Methods: This study consisted of 99 patients with CRSwNP with AERD and CRSwNP without AERD. The RTPCR method and ELISA were used in this study. ELISA was used for MMP9 and TIMP1 serum levels and Vitamin D levels. Results: Serum levels of MMP9 were markedly higher in AG genotype than GG genotype in CRSwNP with AERD. Serum levels of MMP9 were statistically higher in carrying of A  allele in CRSwNP with AERD than in not carrying of A  allele. In addition, serum levels of Vitamin D were discovered significantly lower in TIMP1 CT genotype in comparison to CC and TT genotypes in all of CRSwNP. Conclusion: The rising of MMP9 serum levels may be a marker of chronic inflammation or they may be a significant factor in the resistance to diseases. This study may lead to a further understanding of disease severity in CRSwNP with AERD.

 Objective: Chronic rhinosinusitis with nasal polyposis CRSwNP is a multifactorial disease in which the sinus and nasal mucosa are affected. We investigated the relationship between the genetic polymorphism of MMP9 rs17576 and TIMP1 rs48498 and serum levels, and we wanted to investigate the effect of MMP9 and TIMP1 genotypes on Vitamin serum levels in CRSwNP with and without Aspirin-Exacerbated Respiratory Disease AERD. Materials and Methods: This study consisted of 99 patients with CRSwNP with AERD and CRSwNP without AERD. The RTPCR method and ELISA were used in this study. ELISA was used for MMP9 and TIMP1 serum levels and Vitamin D levels. Results: Serum levels of MMP9 were markedly higher in AG genotype than GG genotype in CRSwNP with AERD. Serum levels of MMP9 were statistically higher in carrying of A  allele in CRSwNP with AERD than in not carrying of A  allele. In addition, serum levels of Vitamin D were discovered significantly lower in TIMP1 CT genotype in comparison to CC and TT genotypes in all of CRSwNP. Conclusion: The rising of MMP9 serum levels may be a marker of chronic inflammation or they may be a significant factor in the resistance to diseases. This study may lead to a further understanding of disease severity in CRSwNP with AERD.

 Objective: Chronic rhinosinusitis with nasal polyposis CRSwNP is a multifactorial disease in which the sinus and nasal mucosa are affected. We investigated the relationship between the genetic polymorphism of MMP9 rs17576 and TIMP1 rs48498 and serum levels, and we wanted to investigate the effect of MMP9 and TIMP1 genotypes on Vitamin serum levels in CRSwNP with and without Aspirin-Exacerbated Respiratory Disease AERD. Materials and Methods: This study consisted of 99 patients with CRSwNP with AERD and CRSwNP without AERD. The RTPCR method and ELISA were used in this study. ELISA was used for MMP9 and TIMP1 serum levels and Vitamin D levels. Results: Serum levels of MMP9 were markedly higher in AG genotype than GG genotype in CRSwNP with AERD. Serum levels of MMP9 were statistically higher in carrying of A  allele in CRSwNP with AERD than in not carrying of A  allele. In addition, serum levels of Vitamin D were discovered significantly lower in TIMP1 CT genotype in comparison to CC and TT genotypes in all of CRSwNP. Conclusion: The rising of MMP9 serum levels may be a marker of chronic inflammation or they may be a significant factor in the resistance to diseases. This study may lead to a further understanding of disease severity in CRSwNP with AERD.

 Amaç: Nazal polipli kronik rinosinüzit sinüs ve nazal mukozanın etkilendiği multifaktöriyel bir hastalıktır. MMP9 rs17576 ve TIMP1 rs 4898 genetik polimorfizmleri ile serum düzeyi arasındaki ilişki ve MMP9 ve TIMP1 genotipleri ve Vitamin D arasındaki bağlantının gösterilmesini araştırdık. Gereç ve Yöntem: Bu çalışma, aspirinle alevlenen solunum hastalığı olan veya olmayan nazal polipozisli kronik rinosinüzitli 99 hastadan oluşmaktadır. RT-PCR ve ELISA metodu kullanılmıştır. MMP9, TIMP1 ve Vitamin D serum seviyeleri için ELISA metodu kullanılmıştır. Bulgular: Aspirinle alevlenen solunum hastalığı eşlik eden nazal polipozisli kronik sinüzit hastalarında MMP9 serum seviyesi, AG genotipli hastalarda GG genotipli hastalara kıyasla daha yüksek seviyededir. Aynı zamanda bu hasta grubunda MMP9 serum seviyesi, A  alleli taşıyan grupta A  alleli taşımayan gruba göre daha yüksek seviyededir. Buna ek olarak TIMP1 CT genotipine sahip olanlar, TIMP1 CC ve TT genotipleri ile kıyaslandığında Vitamin D serum seviyesinin anlamlı olarak düşük olduğu gösterilmiştir. Sonuç: MMP9 serum düzeylerinin yükselmesi, kronik inflamasyonun bir belirteci olabilir veya hastalık direncinde önemli bir faktör olabilir. Bu çalışma, aspirinle alevlenen solunum hastalığı olan nazal polipozisli kronik rinosinüzitte hastalık şiddetinin daha iyi anlaşılmasına yardımcı olabilir.

Article Details

HOW DO THE GENETIC VARIANTS OF MMP 9 AND TIMP 1 AND VITAMIN D AFFECT CHRONIC RHINOSINUSITIS WITH NASAL POLYPOSIS?

Authors:

Meltem Bozacı Kılıçoğlu | Baris Ertugrul | Göksu Kaşarcı | Şenol Çomoğlu | Levent Aydemir | Meryem Nesil Keles Türel | A Gelincik | Bedia Çakmakoğlu

Publisher:

Istanbul University

Main Topics:

Sağlık Kurumları Yönetimi

Journal:

İstanbul Tıp Fakültesi Dergisi Vol.86 Issue 2, pp.152-158

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001287779

pISSN:

1305-6441

Nasal polyposis

MMP9

TIMP1

genetic polymorphism

chronic rhinosinusitisNazal polip

MMP9

TIMP1

genetik polimorfizm

kronik sinüzit

Issues

Article Details

Authors: Meltem Bozacı Kılıçoğlu | Baris Ertugrul | Göksu Kaşarcı | Şenol Çomoğlu | Levent Aydemir | Meryem Nesil Keles Türel | A Gelincik | Bedia Çakmakoğlu

Publisher: Istanbul University

Main Topics: Sağlık Kurumları Yönetimi

Journal: İstanbul Tıp Fakültesi Dergisi Vol.86 Issue 2, pp.152-158

Document Type: Article

Publication Date: 3/2023

Document Language: English

Document ID (Org No.): 2001287779

pISSN: 1305-6441

Issues

Authors:

Meltem Bozacı Kılıçoğlu | Baris Ertugrul | Göksu Kaşarcı | Şenol Çomoğlu | Levent Aydemir | Meryem Nesil Keles Türel | A Gelincik | Bedia Çakmakoğlu

Publisher:

Istanbul University

Main Topics:

Sağlık Kurumları Yönetimi

Journal:

İstanbul Tıp Fakültesi Dergisi Vol.86 Issue 2, pp.152-158

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001287779

pISSN:

1305-6441