INVESTIGATION OF THE EFFECT OF WHEAT GERM OIL ON DIABETES-RELATED TESTICULAR DAMAGE IN TERMS OF OXIDATIVE STRESS PARAMETERS

Aylin Balcı Özyurt; Sezen Yılmaz Sarıaltın

Article Details

INVESTIGATION OF THE EFFECT OF WHEAT GERM OIL ON DIABETES-RELATED TESTICULAR DAMAGE IN TERMS OF OXIDATIVE STRESS PARAMETERS

Authors:

Aylin Balcı Özyurt | Sezen Yılmaz Sarıaltın

Publisher:

Ankara University

Main Topics:

Farmasotik Toksikoloji

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.158-168

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001300956

pISSN:

1015-3918

Keywords:

Abstract:

 Objective: Our study aimed to investigate the oxidative stress-mediated effect of wheat germ oil WGO on testicular damage caused by diabetes. Material and Method: 42 male Wistar albino rats were randomly divided into 6 groups: control, control low-dose 100 mgkgday, control high-dose 1000 mgkgday, diabetes control, diabetes low-dose 100 mgkgday, diabetic high-dose 1000 mgkgday. RYcarrier cornoil was applied to the diabetes and control groups by gavage for 28 days. Blood glucose levels were measured every week. Following euthanasia, testicular tissues were removed. Tissues were homogenized and total protein levels were measured by Bradford method. MDA levels were measured in tissues as an indicator of lipid peroxidation, and glutathione levels were measured as an indicator of oxidative stress. Result and Discussion: Both low and high dose WGO administration in the control group caused a decrease in relative testicular weight. The relative testicular weight of the high dose diabetic group decreased compared to the diabetic control group. There was no significant difference in blood glucose levels and body weights depending on WGO p0.05. Among diabetic animals, the lowest testicular MDA levels were found in the high -dose group. Glutathione levels increased in the control low-dose group. However, there was no significant difference between the groups in terms of MDA and glutathione levels p0.05. Further research is needed to investigate the effectiveness of WGO, which has a traditionally usage and antioxidant activity, on sexual hormones and other molecular pathways.

 Objective: Our study aimed to investigate the oxidative stress-mediated effect of wheat germ oil WGO on testicular damage caused by diabetes. Material and Method: 42 male Wistar albino rats were randomly divided into 6 groups: control, control low-dose 100 mgkgday, control high-dose 1000 mgkgday, diabetes control, diabetes low-dose 100 mgkgday, diabetic high-dose 1000 mgkgday. RYcarrier cornoil was applied to the diabetes and control groups by gavage for 28 days. Blood glucose levels were measured every week. Following euthanasia, testicular tissues were removed. Tissues were homogenized and total protein levels were measured by Bradford method. MDA levels were measured in tissues as an indicator of lipid peroxidation, and glutathione levels were measured as an indicator of oxidative stress. Result and Discussion: Both low and high dose WGO administration in the control group caused a decrease in relative testicular weight. The relative testicular weight of the high dose diabetic group decreased compared to the diabetic control group. There was no significant difference in blood glucose levels and body weights depending on WGO p0.05. Among diabetic animals, the lowest testicular MDA levels were found in the high -dose group. Glutathione levels increased in the control low-dose group. However, there was no significant difference between the groups in terms of MDA and glutathione levels p0.05. Further research is needed to investigate the effectiveness of WGO, which has a traditionally usage and antioxidant activity, on sexual hormones and other molecular pathways.

 Objective: Our study aimed to investigate the oxidative stress-mediated effect of wheat germ oil WGO on testicular damage caused by diabetes. Material and Method: 42 male Wistar albino rats were randomly divided into 6 groups: control, control low-dose 100 mgkgday, control high-dose 1000 mgkgday, diabetes control, diabetes low-dose 100 mgkgday, diabetic high-dose 1000 mgkgday. RYcarrier cornoil was applied to the diabetes and control groups by gavage for 28 days. Blood glucose levels were measured every week. Following euthanasia, testicular tissues were removed. Tissues were homogenized and total protein levels were measured by Bradford method. MDA levels were measured in tissues as an indicator of lipid peroxidation, and glutathione levels were measured as an indicator of oxidative stress. Result and Discussion: Both low and high dose WGO administration in the control group caused a decrease in relative testicular weight. The relative testicular weight of the high dose diabetic group decreased compared to the diabetic control group. There was no significant difference in blood glucose levels and body weights depending on WGO p0.05. Among diabetic animals, the lowest testicular MDA levels were found in the high -dose group. Glutathione levels increased in the control low-dose group. However, there was no significant difference between the groups in terms of MDA and glutathione levels p0.05. Further research is needed to investigate the effectiveness of WGO, which has a traditionally usage and antioxidant activity, on sexual hormones and other molecular pathways.

 Amaç: Ruşeym yağının RY diyabetin neden olduğu testiküler hasar üzerinde oksidatif stres aracılı etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntem: 42 erkek Wistar albino sıçan randomize 6 gruba ayrılmıştır: kontrol, kontrol düşük doz 100 mgkggün, kontrol yüksek doz 1000 mgkggün, diyabet kontrol, diyabet düşük doz 100 mgkggün, diyabet yüksek doz 1000 mgkggün. Diyabet ve kontrol gruplarına 28 gün süre ile gavajla RY uygulanmıştır. Her hafta kan glukoz düzeyleri ölçülmüştür. Ötenazinin ardından testis dokuları çıkartılmıştır. Dokular homojenize edilmiş ve Bradford yöntemi ile total protein düzeyleri ölçülmüştür. Lipit peroksidasyonunun göstergesi olarak dokularda MDA düzeyleri, oksidatif stresin göstergesi olarak glutatyon düzeyleri ölçülmüştür. Sonuç ve Tartışma: Kontrol grubunda hem düşük, hem de yüksek doz RY uygulaması rölatif testis ağırlığında azalmaya neden olmuştur. Diyabetik yüksek doz RY grubunun rölatif testis ağırlığı diyabet kontrol grubuna göre azalmıştır. Kan glukoz düzeyleri ve vücut ağırlıklarında RYye bağlı olarak anlamlı farklılık görülmemiştir p0.05. Diyabetik hayvanlar arasında en düşük testis MDA düzeyleri diyabet yüksek doz grubunda bulunmuştur. Kontrol düşük doz grubunda glutatyon düzeyleri artmıştır. Ancak gruplar arasında MDA ve glutatyon düzeyi bakımından anlamlı farklılık bulunmamıştır p0.05. Halk arasında kullanımı ve antioksidan aktivitesi olan RYnin seksüel hormonlar ve diğer moleküler yolaklar üzerindeki etkisinin araştırılacağı yeni çalışmalara ihtiyaç vardır.

Article Details

INVESTIGATION OF THE EFFECT OF WHEAT GERM OIL ON DIABETES-RELATED TESTICULAR DAMAGE IN TERMS OF OXIDATIVE STRESS PARAMETERS

Authors:

Aylin Balcı Özyurt | Sezen Yılmaz Sarıaltın

Publisher:

Ankara University

Main Topics:

Farmasotik Toksikoloji

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.158-168

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001300956

pISSN:

1015-3918

Germ oil

oxidative stress

testicular damage

wheat germBuğday ruşeymi

oksidatif stres

ruşeym yağı

testiküler hasar

Issues

Article Details

Authors: Aylin Balcı Özyurt | Sezen Yılmaz Sarıaltın

Publisher: Ankara University

Main Topics: Farmasotik Toksikoloji

Journal: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.158-168

Document Type: Article

Publication Date: 1/2024

Document Language: Turkish

Document ID (Org No.): 2001300956

pISSN: 1015-3918

Issues

Authors:

Aylin Balcı Özyurt | Sezen Yılmaz Sarıaltın

Publisher:

Ankara University

Main Topics:

Farmasotik Toksikoloji

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.158-168

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001300956

pISSN:

1015-3918