Determine the Microbial Flora of Leeches in North of Iran and Designing Antimicrobial  Solution to Sterile the Leeches

Semiramis JABERI; Mehdi RAHNEMA; Reza SHAPOURI

Article Details

Determine the Microbial Flora of Leeches in North of Iran and Designing Antimicrobial Solution to Sterile the Leeches

Authors:

Semiramis JABERI | Mehdi RAHNEMA | Reza SHAPOURI

Publisher:

Nobel Bilim ve Araştırma Merkezi Limited

Journal:

Journal of Applied Biological Sciences 2014 Vol.8 Issue 3, pp.42-45

Document Type:

Article

Publication Date:

10/2014

Document Language:

Turkish

Document ID (Org No.):

2000831293

Frequency:

Tri-annual

eISSN:

2146-0108

Abstract:

 

Medicinal leeches have an important and expanding role in plastic surgery and in the management of chronic venous insufficiency, but infection can complicate their use. The aim of this study were designed a new protocol to sterilize or at least to reduce the number of colonies of the bacteria on medical leeches. Bacterial flora of the surface, mouth, and gut of medical leeches was studied and bacterial identification was conducted based on morphological and biochemical tests. The susceptibilities of these isolates to 11 antimicrobial agents were investigated. Next, the leeches were immersed in susceptible antibiotic and the final results were studied. Biochemical and morphological tests indicated that Aeromonasspp.is the dominant culturablesymbiont in leeches collected from north of Iran. Isolates were highly susceptible to tetracycline, gentamicin, cotrimoxazole and ceftriaxone. According to results, tetracycline was superior to other antibiotic to cleansing of bacteria from leeches. Aeromonasspp.were eliminated completely from all leeches and statistically significant cleaning was obtained with the use of tetracycline and gentamicin solution after 4 days for 5.5 h daily.

Article Details

Determine the Microbial Flora of Leeches in North of Iran and Designing Antimicrobial Solution to Sterile the Leeches

Authors: Semiramis JABERI | Mehdi RAHNEMA | Reza SHAPOURI

Publisher: Nobel Bilim ve Araştırma Merkezi Limited

Journal: Journal of Applied Biological Sciences 2014 Vol.8 Issue 3, pp.42-45

Document Type: Article

Publication Date: 10/2014

Document Language: Turkish

Document ID (Org No.): 2000831293

Frequency: Tri-annual

eISSN: 2146-0108

Issues

Authors:

Semiramis JABERI | Mehdi RAHNEMA | Reza SHAPOURI

Publisher:

Nobel Bilim ve Araştırma Merkezi Limited

Journal:

Journal of Applied Biological Sciences 2014 Vol.8 Issue 3, pp.42-45

Document Type:

Article

Publication Date:

10/2014

Document Language:

Turkish

Document ID (Org No.):

2000831293

Frequency:

Tri-annual

eISSN:

2146-0108