Evaluation of Toxic Effects of Valeriana alliariifolia Adams Root Extract on SH-SY5Y and PC-12 Cell Lines and Investigation of Antioxidant Activity

Selen İlgün; Gökçe Şeker Karatoprak

Article Details

Evaluation of Toxic Effects of Valeriana alliariifolia Adams Root Extract on SH-SY5Y and PC-12 Cell Lines and Investigation of Antioxidant Activity

Authors:

Selen İlgün | Gökçe Şeker Karatoprak

Publisher:

Igdir University

Main Topics:

Bitki Bilimi (Diğer)

Journal:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 1, pp.83-91

Document Type:

Article

Publication Date:

1/2025

Document Language:

Turkish

Document ID (Org No.):

2001341058

pISSN:

2536-4618

Keywords:

Abstract:

 The research examined the toxicity of 80 ethanol extract prepared from the roots of the Valeriana alliariifolia Adams species grown in Turkiye on SH-SY5Y neuroblastoma and neuron-like PC-12 cell lines. The total phenol and flavonoid amounts of the extract were determined by spectrophotometric methods. Antioxidant activity was analyzed by DPPH and ABTS radical scavenging activity determination methods. It was determined that the total phenol content of the extract was 210.422.58 mgGAEgextract and that it could strongly scavenge the DPPH radical in the concentration range of 0.2-4 mgmL. It has been observed that the extract did not have a toxic effect on SH-SY5Y and PC-12 cells at low concentrations 3.9 and 7.81 µgmL and also had lower toxicity on the PC-12 cell line with an IC50 value of 369.68  2.61 µgmL. The results indicate that Valeriana alliariifolia species may be potential candidates for developing new phytopharmaceuticals and functional ingredients..

 The research examined the toxicity of 80 ethanol extract prepared from the roots of the Valeriana alliariifolia Adams species grown in Turkiye on SH-SY5Y neuroblastoma and neuron-like PC-12 cell lines. The total phenol and flavonoid amounts of the extract were determined by spectrophotometric methods. Antioxidant activity was analyzed by DPPH and ABTS radical scavenging activity determination methods. It was determined that the total phenol content of the extract was 210.422.58 mgGAEgextract and that it could strongly scavenge the DPPH radical in the concentration range of 0.2-4 mgmL. It has been observed that the extract did not have a toxic effect on SH-SY5Y and PC-12 cells at low concentrations 3.9 and 7.81 µgmL and also had lower toxicity on the PC-12 cell line with an IC50 value of 369.68  2.61 µgmL. The results indicate that Valeriana alliariifolia species may be potential candidates for developing new phytopharmaceuticals and functional ingredients..

 The research examined the toxicity of 80 ethanol extract prepared from the roots of the Valeriana alliariifolia Adams species grown in Turkiye on SH-SY5Y neuroblastoma and neuron-like PC-12 cell lines. The total phenol and flavonoid amounts of the extract were determined by spectrophotometric methods. Antioxidant activity was analyzed by DPPH and ABTS radical scavenging activity determination methods. It was determined that the total phenol content of the extract was 210.422.58 mgGAEgextract and that it could strongly scavenge the DPPH radical in the concentration range of 0.2-4 mgmL. It has been observed that the extract did not have a toxic effect on SH-SY5Y and PC-12 cells at low concentrations 3.9 and 7.81 µgmL and also had lower toxicity on the PC-12 cell line with an IC50 value of 369.68  2.61 µgmL. The results indicate that Valeriana alliariifolia species may be potential candidates for developing new phytopharmaceuticals and functional ingredients..

 Araştırmada, Türkiyede yetişen Valeriana alliariifolia Adams türünün köklerinden hazırlanan 80lik etanol ekstresinin SH-SY5Y nöroblastoma ve nöron benzeri PC-12 hücre hatlarındaki toksisitesi incelenmiştir. Ekstrenin toplam fenol ve flavonoit miktarı spektrofotometrik yöntemlerle belirlenmiştir. Antioksidan aktivitesi, DPPH ve ABTS radikal süpürücü aktivite tayin yöntemleri ile analiz edilmiştir. Ekstrenin, toplam fenol içeriğinin 210.422.58 mgGAEgekstre olduğu, 0.2-4 mgmL konsantrasyon aralığında ise DPPH radikalini güçlü bir şekilde süpürebildiği belirlenmiştir. Ekstrenin düşük 3.9 ve 7.81 µgmL konsantrasyonlarda SH-SY5Y ve PC-12 hücreleri üzerinde toksik etki göstermediği, aynı zamanda 369.68  2.61 µgmL IC50 değeri ile PC-12 hücre hattında daha düşük toksisiteye sahip olduğu gözlenmiştir. Sonuçlar, Valeriana alliariifolia türünün yeni fitofarmasötikler ve fonksiyonel bileşenler geliştirmek için potansiyel aday olabileceğini göstermektedir.

Article Details

Evaluation of Toxic Effects of Valeriana alliariifolia Adams Root Extract on SH-SY5Y and PC-12 Cell Lines and Investigation of Antioxidant Activity

Authors:

Selen İlgün | Gökçe Şeker Karatoprak

Publisher:

Igdir University

Main Topics:

Bitki Bilimi (Diğer)

Journal:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 1, pp.83-91

Document Type:

Article

Publication Date:

1/2025

Document Language:

Turkish

Document ID (Org No.):

2001341058

pISSN:

2536-4618

Valeriana alliariifolia

antioxidants

pc-12

sh-sy5yValeriana alliariifolia

antioksidan

pc-12

sh-sy5y

Issues

Article Details

Authors: Selen İlgün | Gökçe Şeker Karatoprak

Publisher: Igdir University

Main Topics: Bitki Bilimi (Diğer)

Journal: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 1, pp.83-91

Document Type: Article

Publication Date: 1/2025

Document Language: Turkish

Document ID (Org No.): 2001341058

pISSN: 2536-4618

Issues

Authors:

Selen İlgün | Gökçe Şeker Karatoprak

Publisher:

Igdir University

Main Topics:

Bitki Bilimi (Diğer)

Journal:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.15 Issue 1, pp.83-91

Document Type:

Article

Publication Date:

1/2025

Document Language:

Turkish

Document ID (Org No.):

2001341058

pISSN:

2536-4618