The Effect of Different Insemination Doses on Pregnancy Success in East Frisian Sheep

Ahmet Aktar; Hasan Özkan; Kemal Bağcı; Mustafa Akkaşoğlu; Hakan Sağırkaya; Selim Alçay

Article Details

The Effect of Different Insemination Doses on Pregnancy Success in East Frisian Sheep

Authors:

Ahmet Aktar | Hasan Özkan | Kemal Bağcı | Mustafa Akkaşoğlu | Hakan Sağırkaya | Selim Alçay

Publisher:

Bursa Uludağ University

Main Topics:

Veterinary Surgery

Journal:

Journal of Research in Veterinary Medicine Vol.42 Issue 1, pp.32-36

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001296816

pISSN:

2667-6745

Keywords:

Abstract:

 Semen collection and insemination under field conditions may not always be done in similar and clean conditions. In this study, it was aimed to investigate the pregnancy rates as a result of intracervical artificial insemination at three different doses of fresh semen under field conditions. In the research, 60 East Frisian sheep were divided into 3 groupsn20 and 3 rams were used. In order to synchronize the estrus in sheep, sponges impregnated with 20 mg flugestone acetate were placed in the all groups for 12 days. All sheep were injected with 500 IU PMSG to synchronize ovulation on the day the sponges were to be removed, and it was determined that all sheep showed oestrus within 24 hours. Sheep were inseminated intracervically with semen from 3 rams of the same breed in the same farm. Insemination doses were determined as 100x106 sptml for the first group, 200x106 sptml for the second group and 400x106 sptml for the third group. After insemination, it was determined that 28 43 sheep did not have fertilization and showed heat again, and the other 32 53 sheep became pregnant. According to the statistical analysis results, the 3rd group 400x106 was found to be significantly more successful than the 1st group 100x106 P0.05. As a result, when the insemination doses were compared, it was determined that insemination with 400x106 spermatozoaml diluted fresh semen was more effective in obtaining the high pregnancy rates.

 Semen collection and insemination under field conditions may not always be done in similar and clean conditions. In this study, it was aimed to investigate the pregnancy rates as a result of intracervical artificial insemination at three different doses of fresh semen under field conditions. In the research, 60 East Frisian sheep were divided into 3 groupsn20 and 3 rams were used. In order to synchronize the estrus in sheep, sponges impregnated with 20 mg flugestone acetate were placed in the all groups for 12 days. All sheep were injected with 500 IU PMSG to synchronize ovulation on the day the sponges were to be removed, and it was determined that all sheep showed oestrus within 24 hours. Sheep were inseminated intracervically with semen from 3 rams of the same breed in the same farm. Insemination doses were determined as 100x106 sptml for the first group, 200x106 sptml for the second group and 400x106 sptml for the third group. After insemination, it was determined that 28 43 sheep did not have fertilization and showed heat again, and the other 32 53 sheep became pregnant. According to the statistical analysis results, the 3rd group 400x106 was found to be significantly more successful than the 1st group 100x106 P0.05. As a result, when the insemination doses were compared, it was determined that insemination with 400x106 spermatozoaml diluted fresh semen was more effective in obtaining the high pregnancy rates.

 Semen collection and insemination under field conditions may not always be done in similar and clean conditions. In this study, it was aimed to investigate the pregnancy rates as a result of intracervical artificial insemination at three different doses of fresh semen under field conditions. In the research, 60 East Frisian sheep were divided into 3 groupsn20 and 3 rams were used. In order to synchronize the estrus in sheep, sponges impregnated with 20 mg flugestone acetate were placed in the all groups for 12 days. All sheep were injected with 500 IU PMSG to synchronize ovulation on the day the sponges were to be removed, and it was determined that all sheep showed oestrus within 24 hours. Sheep were inseminated intracervically with semen from 3 rams of the same breed in the same farm. Insemination doses were determined as 100x106 sptml for the first group, 200x106 sptml for the second group and 400x106 sptml for the third group. After insemination, it was determined that 28 43 sheep did not have fertilization and showed heat again, and the other 32 53 sheep became pregnant. According to the statistical analysis results, the 3rd group 400x106 was found to be significantly more successful than the 1st group 100x106 P0.05. As a result, when the insemination doses were compared, it was determined that insemination with 400x106 spermatozoaml diluted fresh semen was more effective in obtaining the high pregnancy rates.

 Saha şartlarında sperma almak ve tohumlama yapmak her zaman aynı koşullarda ve temiz şartlarda yapılamamaktadır. Bu çalışmada saha şartlarında üç farklı dozda taze sperma ile intraservikal yolla suni tohumlama uygulaması sonucunda gebelik oranlarının araştırılması amaçlandı. Araştırmada Doğu Friz ırkı 60 adet koyun 3 gruba ayrıldı n20 ve 3 adet koç kullanıldı. Koyunlarda kızgınlıkların senkronize edilmesi amacıyla 20 mg flugeston asestat emdirilmiş süngerler 3 gruba da 12 gün süreyle yerleştirildi. Süngerlerin çıkarılacağı gün ovulasyonları senkronize etmek için tüm koyunlara 500 IU PMSG enjeksiyonu yapıldı ve 24 saat içerisinde tüm koyunların östrus gösterdiği tespit edildi. Aynı çiftlikteki aynı ırk 3 koçtan alınan spermalarla östrustaki koyunlar intraservikal yolla tohumlandı. Tohumlama dozları 1.grup için 100x106 spermatozoaml, 2.grup için 200x106 spermatozoaml, 3.grup için 400x106 spermatozoaml olarak belirlendi. Tohumlamaların ardından toplamda 28 adet 47 koyunda fertilizasyon olmadığı ve tekrar kızgınlık gösterdiği, diğer 32 adet 53 koyunun gebe kaldığı tespit edildi. Gerçekleştirilen istatistiksel analiz sonucunda 3.Grup 400x106 gebelik oranı 1. Gruba 100x106 göre anlamlı olarak daha başarılı bulunmuştur P0.05. Sonuç olarak tohumlama dozları kıyaslandığında saha koşullarında 400x106 spermatozoaml dozda sulandırılmış taze sperma ile tohumlamanın yüksek gebelik oranlarının elde edilmesinde etkili olduğu belirlendi.

Article Details

The Effect of Different Insemination Doses on Pregnancy Success in East Frisian Sheep

Authors:

Ahmet Aktar | Hasan Özkan | Kemal Bağcı | Mustafa Akkaşoğlu | Hakan Sağırkaya | Selim Alçay

Publisher:

Bursa Uludağ University

Main Topics:

Veterinary Surgery

Journal:

Journal of Research in Veterinary Medicine Vol.42 Issue 1, pp.32-36

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001296816

pISSN:

2667-6745

East Frieze

Sheep

Sperma

Synchronization

PMSG

Progestagen

Doğu Friz

Koyun

Senkronizasyon

ProgestajenDoğu Friz

Koyun

Sperma

Senkronizasyon

PMSG

Progestagen

Issues

Article Details

Authors: Ahmet Aktar | Hasan Özkan | Kemal Bağcı | Mustafa Akkaşoğlu | Hakan Sağırkaya | Selim Alçay

Publisher: Bursa Uludağ University

Main Topics: Veterinary Surgery

Journal: Journal of Research in Veterinary Medicine Vol.42 Issue 1, pp.32-36

Document Type: Article

Publication Date: 1/2023

Document Language: Turkish

Document ID (Org No.): 2001296816

pISSN: 2667-6745

Issues

Authors:

Ahmet Aktar | Hasan Özkan | Kemal Bağcı | Mustafa Akkaşoğlu | Hakan Sağırkaya | Selim Alçay

Publisher:

Bursa Uludağ University

Main Topics:

Veterinary Surgery

Journal:

Journal of Research in Veterinary Medicine Vol.42 Issue 1, pp.32-36

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001296816

pISSN:

2667-6745