Reliability of antibody tests for COVID-19 diagnosis

N.A.

Article Details

Reliability of antibody tests for COVID-19 diagnosis

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Clinical Sciences

Journal:

Marmara Medical Journal Vol.36 Issue 1, pp.93-98

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283565

pISSN:

1019-1941

Keywords:

Abstract:

 Objective: The reverse transcriptionpolymerase chain reaction test RT-PCR is the gold standard for the diagnosis of coronavirus disease 2019 COVID-19, and antibody tests are useful as supplemental tools for diagnosis, for measuring the populations immunity levels, and for checking infection in asymptomatic contacts. This study aimed to evaluate the reliability of five commercial antibody detection test kits. Materials and Methods: The reliability of the Colloidal Gold COVID-19 IgGIgM Rapid Test Kit, Antibody Rapid Test Hotgen, Beijing Hotgen Biotech Co., Ltd., China, Abbott Chemiluminescent Microparticle Immunoassay Illinois, USA, Roche Electrochemiluminescence Immunoassay Roche Diagnostics, Switzerland, Siemens Chemiluminescence Munich, Germany, and Euroimmun ELISA Lübeck, Germany for COVID-19 diagnosis was studied. The antibody-negative group included 50 sera from 2018, and the antibody-positive group included 98 patients with positive RT-PCR results from whom blood samples had been collected 39 weeks after hospital discharge. Statistical analysis was performed using SPSS version 23.0 IBM Corporation, Armonk, NY, USA. The antibody tests validity and intra-assay reproducibility were examined, and the Cohens kappa coefficients were obtained. The disease prevalence was pegged at 10. Results: The antibody tests sensitivity 69.1272.46 and positive predictive values 42.44100.0 were low, and their specificity 89.58100 and negative predictive values 96.3197.03 were high. Their accuracy rates varied from 87.54 to 97.25, and their intra-assay coefficients of variation varied from 1 to 10. Conclusion: The agreement between the results of the antibody detection test kits was higher when the kits were classified according to the targeted antigens. The time of blood sample collection, targeted antigens, and antibody types affected the results. Serological tests were found to be useful, and the commercial kits were found to be largely reliable, although, some parameters need to be improved.

 Objective: The reverse transcriptionpolymerase chain reaction test RT-PCR is the gold standard for the diagnosis of coronavirus disease 2019 COVID-19, and antibody tests are useful as supplemental tools for diagnosis, for measuring the populations immunity levels, and for checking infection in asymptomatic contacts. This study aimed to evaluate the reliability of five commercial antibody detection test kits. Materials and Methods: The reliability of the Colloidal Gold COVID-19 IgGIgM Rapid Test Kit, Antibody Rapid Test Hotgen, Beijing Hotgen Biotech Co., Ltd., China, Abbott Chemiluminescent Microparticle Immunoassay Illinois, USA, Roche Electrochemiluminescence Immunoassay Roche Diagnostics, Switzerland, Siemens Chemiluminescence Munich, Germany, and Euroimmun ELISA Lübeck, Germany for COVID-19 diagnosis was studied. The antibody-negative group included 50 sera from 2018, and the antibody-positive group included 98 patients with positive RT-PCR results from whom blood samples had been collected 39 weeks after hospital discharge. Statistical analysis was performed using SPSS version 23.0 IBM Corporation, Armonk, NY, USA. The antibody tests validity and intra-assay reproducibility were examined, and the Cohens kappa coefficients were obtained. The disease prevalence was pegged at 10. Results: The antibody tests sensitivity 69.1272.46 and positive predictive values 42.44100.0 were low, and their specificity 89.58100 and negative predictive values 96.3197.03 were high. Their accuracy rates varied from 87.54 to 97.25, and their intra-assay coefficients of variation varied from 1 to 10. Conclusion: The agreement between the results of the antibody detection test kits was higher when the kits were classified according to the targeted antigens. The time of blood sample collection, targeted antigens, and antibody types affected the results. Serological tests were found to be useful, and the commercial kits were found to be largely reliable, although, some parameters need to be improved.

 Objective: The reverse transcriptionpolymerase chain reaction test RT-PCR is the gold standard for the diagnosis of coronavirus disease 2019 COVID-19, and antibody tests are useful as supplemental tools for diagnosis, for measuring the populations immunity levels, and for checking infection in asymptomatic contacts. This study aimed to evaluate the reliability of five commercial antibody detection test kits. Materials and Methods: The reliability of the Colloidal Gold COVID-19 IgGIgM Rapid Test Kit, Antibody Rapid Test Hotgen, Beijing Hotgen Biotech Co., Ltd., China, Abbott Chemiluminescent Microparticle Immunoassay Illinois, USA, Roche Electrochemiluminescence Immunoassay Roche Diagnostics, Switzerland, Siemens Chemiluminescence Munich, Germany, and Euroimmun ELISA Lübeck, Germany for COVID-19 diagnosis was studied. The antibody-negative group included 50 sera from 2018, and the antibody-positive group included 98 patients with positive RT-PCR results from whom blood samples had been collected 39 weeks after hospital discharge. Statistical analysis was performed using SPSS version 23.0 IBM Corporation, Armonk, NY, USA. The antibody tests validity and intra-assay reproducibility were examined, and the Cohens kappa coefficients were obtained. The disease prevalence was pegged at 10. Results: The antibody tests sensitivity 69.1272.46 and positive predictive values 42.44100.0 were low, and their specificity 89.58100 and negative predictive values 96.3197.03 were high. Their accuracy rates varied from 87.54 to 97.25, and their intra-assay coefficients of variation varied from 1 to 10. Conclusion: The agreement between the results of the antibody detection test kits was higher when the kits were classified according to the targeted antigens. The time of blood sample collection, targeted antigens, and antibody types affected the results. Serological tests were found to be useful, and the commercial kits were found to be largely reliable, although, some parameters need to be improved.

 Objective: The reverse transcriptionpolymerase chain reaction test RT-PCR is the gold standard for the diagnosis of coronavirus disease 2019 COVID-19, and antibody tests are useful as supplemental tools for diagnosis, for measuring the populations immunity levels, and for checking infection in asymptomatic contacts. This study aimed to evaluate the reliability of five commercial antibody detection test kits. Materials and Methods: The reliability of the Colloidal Gold COVID-19 IgGIgM Rapid Test Kit, Antibody Rapid Test Hotgen, Beijing Hotgen Biotech Co., Ltd., China, Abbott Chemiluminescent Microparticle Immunoassay Illinois, USA, Roche Electrochemiluminescence Immunoassay Roche Diagnostics, Switzerland, Siemens Chemiluminescence Munich, Germany, and Euroimmun ELISA Lübeck, Germany for COVID-19 diagnosis was studied. The antibody-negative group included 50 sera from 2018, and the antibody-positive group included 98 patients with positive RT-PCR results from whom blood samples had been collected 39 weeks after hospital discharge. Statistical analysis was performed using SPSS version 23.0 IBM Corporation, Armonk, NY, USA. The antibody tests validity and intra-assay reproducibility were examined, and the Cohens kappa coefficients were obtained. The disease prevalence was pegged at 10. Results: The antibody tests sensitivity 69.1272.46 and positive predictive values 42.44100.0 were low, and their specificity 89.58100 and negative predictive values 96.3197.03 were high. Their accuracy rates varied from 87.54 to 97.25, and their intra-assay coefficients of variation varied from 1 to 10. Conclusion: The agreement between the results of the antibody detection test kits was higher when the kits were classified according to the targeted antigens. The time of blood sample collection, targeted antigens, and antibody types affected the results. Serological tests were found to be useful, and the commercial kits were found to be largely reliable, although, some parameters need to be improved.

Article Details

Reliability of antibody tests for COVID-19 diagnosis

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Clinical Sciences

Journal:

Marmara Medical Journal Vol.36 Issue 1, pp.93-98

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283565

pISSN:

1019-1941

COVID-19

Antibody

Validity

Chemiluminescent

Electrochemiluminescence

ELISA

COVID-19

Antibody

Validity

Chemiluminescent

Electrochemiluminescence

ELISA

Issues

Article Details

Authors: N.A.

Publisher: Marmara University

Main Topics: Clinical Sciences

Journal: Marmara Medical Journal Vol.36 Issue 1, pp.93-98

Document Type: Article

Publication Date: 1/2023

Document Language: English

Document ID (Org No.): 2001283565

pISSN: 1019-1941

Issues

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Clinical Sciences

Journal:

Marmara Medical Journal Vol.36 Issue 1, pp.93-98

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283565

pISSN:

1019-1941