ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia

Sunarno SUNARNO; Yunı RUKMINIATI; Ratıh Dıan SARASWATI

doi:https://doi.org/10.3906/sag-1909-4

Article Details

ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia

Authors:

Sunarno SUNARNO | Yunı RUKMINIATI | Ratıh Dıan SARASWATI

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2020 Vol.50 Issue 1, pp.267-270

Document Type:

Article

Publication Date:

12/2019

Document Language:

English

Document ID (Org No.):

2000749731

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

DOI:

https://doi.org/10.3906/sag-1909-4

Abstract:

 

Background/aim:  The  aim  of  this  study  was  to  find  out  characteristics  and  patterns  of  the  spread  of  Corynebacterium  diphtheriaeisolated from Jakarta and the surrounding areas, using the whole genome sequencing  WGS  technique and multilocus sequence typing  MLST  approach.Materials and methods: The study samples consisted of 86 C. diphtheriae isolates, which were isolated from diphtheria patients and close contacts of patients. The DNA sequencing was carried out using the WGS technique. Data conversion applied the U-gene software. Molecular typing was conducted through the MLST approach, then followed by online data analysis. Results: The results showed that as many as 43  50%  of all samples examined were new types with the same allele profile, namely 9-1-13-4-3-3-4. New sequence type C. diphtheriae is registered in the MLST global database as ST534 based on the allele profile. The tox gene analysis in 43 isolates with ST534 indicated that there were three mutation positions, all of which were silent mutations.Conclusion: The main cause of diphtheria in Jakarta and the surrounding areas is a new sequence type of C. diphtheriae registered as ST534.

Article Details

ST534: the new sequence type of Corynebacterium diphtheriae causing diphtheria in Jakarta and surrounding areas, Indonesia

Authors: Sunarno SUNARNO | Yunı RUKMINIATI | Ratıh Dıan SARASWATI

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2020 Vol.50 Issue 1, pp.267-270

Document Type: Article

Publication Date: 12/2019

Document Language: English

Document ID (Org No.): 2000749731

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

DOI: https://doi.org/10.3906/sag-1909-4

Issues

Authors:

Sunarno SUNARNO | Yunı RUKMINIATI | Ratıh Dıan SARASWATI

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2020 Vol.50 Issue 1, pp.267-270

Document Type:

Article

Publication Date:

12/2019

Document Language:

English

Document ID (Org No.):

2000749731

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

DOI:

https://doi.org/10.3906/sag-1909-4